Báo cáo y học: " Antigenic analysis of classical swine fever virus E2 glycoprotein using pig antibodies identifies residues contributing to antigenic variation of the vaccine C-strain and group 2 strains circulating in China" ppt
Báo cáo y học: " Antigenic analysis of classical swine fever virus E2 glycoprotein using pig antibodies identifies residues contributing to antigenic variation of the vaccine C-strain and group 2 strains circulating in China" ppt
... wt C-strain rE2 protein binding to pig anti -C-strain and anti-QZ-07 sera,respectively. Thus, the binding of the wt C-strain rE2 protein to either of the sera was set at 100%. rE2-BC proteins ... binding efficiency of individual rE2 proteins. For each of the variant C-strain rE2 proteins, the binding efficiency wasdetermined by normalizing to anti-his-tag binding first, and then to the ... between CSFV vaccine C-strain and subgroup 2. 1 strains, but also among strains of the same subgroup 2. 1 as determined by ELISA-based bindingassay using pig antisera to the C-strain and a representative...
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Báo cáo y học: "Sequence analysis of the Epstein-Barr virus (EBV) BRLF1 gene in nasopharyngeal and gastric carcinomas" docx
... Epstein-Barr virus immediate-early proteins BZLF1 and BRLF1activate the ATF2 transcription factor by increasing the levels of phosphorylated p38 and c-Jun N-terminal kinases. J Virol 20 00,74: 122 4- 123 3.19. ... ScienceFoundation of China (NSFC 30740068 and NSFC 30970157); the NaturalScience Foundation of Shandong Province, China (Y2 008C90); Science and Technology of Qingdao City, China (08 -2- 3-7-hz and 08 -2- 1-4-nsh).Author ... in the lytic activation. DNA binding domain wasmainly affected by muta tion R®M at residue 27 3 and K®E at residue 316 and these changes were signifi-cantly higher in NPC. R®Matresidue273maybeofgreat...
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Báo cáo y học: " Functional analysis of human T lymphotropic virus type 2 Tax proteins" pptx
... signal, zinc finger domain and morerecently a domain in Tax 2 between amino acids 90–100shown to be involved in the cytoplasmic location of Tax 2 proteins [13,14,16 ,22 ]. However analysis of the ... promoters and similar to its effects on Tax 1 Y1 44C appeared to only reduce the activity of Tax 2B. However the introduction of anarginine instead of a cysteine at this position (Y1 44R) intoTax 2B ... L87I and P92L are close to a domain previously found to be involved in the cyto-plasmic localization of Tax 2 proteins (Figure 3A) [19 ,22 ].W248R and Y1 44C are located in the central region of...
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Báo cáo y học: "Detailed analysis of 15q11-q14 sequence corrects errors and gaps in the public access sequence to fully reveal large segmental duplications at breakpoints for Prader-Willi, Angelman, and inv dup(15) syndromes" pdf
... 1b) for the duplicon covered by the two adjacentends of NT_01 028 0 and NT_078096. The second two groups(haplotigs 2a and 2b) therefore cover the other duplicon,including the beginning of NT_078094. The ... reaching the end of segment Y and therefore lacking segment P. Sequence analysis of the above clones containing haplotigs 2a and 2b showed thatRP11-989M14 (GenBank: AC 121 153) and RP11- 128 1C 22 (GenBank: ... a history of psychosis. Am J Psychiatry 20 05, 1 62: 43-49.13. Weiland S, Bertrand D, Leonard S: Neuronal nicotinic acetylcho-line receptors: from the gene to the disease. Behav Brain Res 20 00,...
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Báo cáo y học: " Intracytoplasmic maturation of the human immunodeficiency virus type 1 reverse transcription complexes determines their capacity to integrate into chromatin" pot
... and staying on the pathway to integration. In vitro integration of HIV-1 PICs into isolated chromatin To compare integrative capacity of cytoplasmic and nuclear complexes, and to evaluate the effect ... (PICs),trafficking of complexes into the nucleus, and finally integration of the viral DNA into chromatin.Molecular details and temporal organization of these processes remain among the least investigated and ... PCR analysis of ERT activity and integration of cytoplasmic and nuclear RTCs. A. ERT activity of cRTCs and nRTCs isolated 2 h and 5 h post-infection. cRTCs and nRTCs were normalized according to...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... showed inhibitory effects on the proapoptotic proteins Bid and Bad. Bag-1 also exhibited apoptotic effects, probably by inhibiting the expression of Bcl -2 and by modulating phosphorylation of ... experimental sample to control detected by using the Odyssey Infrared Imaging System. (+) means an increase of signal intensity, and (-) means a decrease of signal intensity after exposure to hypoxia. ... changes in the proteins related to apoptosis during hypoxia to clarify the mechanism of hypoxia-induced apoptosis, and to find out useful hypoxia-related markers of trophoblastic cells. 2. Materials...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
... Biochemistry of the Charité, Berlin, Germany using the ABI PRISM Big Dye Terminator system (Applied Biosystems, Germany). Sequences were analyzed using the Chromas software (Technely-sium Pty Ltd, ... samples containing the C/T SNP downstream of exon 10 led to the identification of cleavage products of approximately 101 bp and 444 bp in size in samples either containing the heterozygous C/T ... intronic SNP During the analysis of exon 10 and adjacent intronic sequences we could for the first time identify a new single nucleotide polymorphism (SNP) in the intron sequence flanking...
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Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"
... regulatory sub-unit 2 Cks2 33197 Mm .22 222 8 -3 .27 -3 .26 -2. 76 Mus musculus cytochrome C oxidase subunit VIIb mRNA [NM_ 025 379] Cox7b 661 42 Mm.197 728 -1. 92 -3.39 -2. 83 Integrin beta 3 binding ... (Figure 5). These genes thus may be a contrib-uting factor to the increase in B cells, both in total number and in maturity, within the thymus. The increase in B cell numbers in the thymus with ... samples and blue lines the old samples. Flow cy-tometry demonstrates that there is a clear shift in the mean fluorescence intensity of IgM in the old thymocytes when compared to the young thymocytes....
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... in the inhibitor structure. In addition to its PLA 2 inhibitoryactivity against the basic myotoxins I and III from B. aspervenom, BaMIP also inhibited the myotoxic, edematogenic and cytolytic ... were determined by the Lowry method [26 ] using BSA as a standard. Routinely, in all inhibition assays, the toxins and the inhibitor were mixed and incubated for30 min at 37 °C.Polyacrylamide gel ... inducedby myotoxins I (Asp49) and II (Lys49) from B. asper venomwas analyzed in vivo and in vitro. DM64 effectively neut-ralized the myotoxic and cytotoxic effects of both myotox-ins. Interestingly,...
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Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx
... the othertruncated derivatives of p26, the induction of thermotoler-ance by p26-ND60 and p26-alpha did not depend onoligomer formation.Fig. 2. Expression of pET21(+) containing p26 cDNA in ... shock/a-crystallin proteins confer thermotol-erance on prokaryotic and eukaryotic organisms [23 ,27 –33]. The construct used p reviously to examineinduction of thermotolerance by full-length Artemia p26encoded ... p26-CD10, lacks C-terminal residues 183–1 92; p26-alpha, lacks residues 1–60 and 153–1 92, thereby corresponding to t he a-crystallin domain. That the cDNA fragments in pET21(+) were of the proper sizewas...
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