Báo cáo y học: " An RNAi in silico approach to find an optimal shRNA cocktail against HIV-1" potx

... limits. In any case, targeting the dominant and subdominant viral variants for each region may reduce this set of viable changes. We did not find any other genome region to be tar- geted, probably ... variants in several conserved regions instead of identifying a single consensus sequence for each, in ordertobeabletousethemallsimultaneouslyina combination cocktail. These variants w...

Ngày tải lên: 11/08/2014, 21:21

... purposes) consisting mainly of HSP's, actin and tubulin. Only 10% of these proteins were shared with the S-Tax-GFP experi- ments. One approach to assigning value to any single protein- protein interaction ... Our analysis suggested a starting point of four confirmed Tax- binding proteins; Rad51, TOP1, Chk2, and 53BP1. Construction of a physical Tax interactome map Our approach t...

Ngày tải lên: 13/08/2014, 05:21

... actin depolymerizing factor (ADF)/cofilin family of actin-binding proteins and it has been proposed that there is a similarity in the actin-binding interface. Gelsolin domains 1 and 4 bind G-actin in ... with ADF/cofilin. We present a model for F-actin binding of domain 2 with respect to the F-actin severing and capping activity of the whole gelsolin molecule. Keywords: actin; actin-bindi...

Ngày tải lên: 22/02/2014, 07:20

... regulatory protein may bind to the upstream regulatory element constituting a protein– DNA interaction [4–6]. The availability of the regulatory proteintointeractwithDNAmaydependonitsactivity, which ... obtained by in vitro studies and represent constraints on the candidate models. In this work, we analyze the GAL genetic switch in S. cerevisiae to demonstrate such an approach t...

Ngày tải lên: 07/03/2014, 16:20

... Rothstein JM: Measurement and Clinical Practice: The Theory and Application. In Measurement in Physical Therapy Edited by: Rothstein JM. New York: Churchill Livingstone; 1985:1. 19. Bland JM, Altman ... bearing and weight bearing was 10 millimeters. The change in arch height during the Sit -to- Stand test was shown to have good to high levels of intra- and inter-reliability as well...

Ngày tải lên: 10/08/2014, 21:23

... Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and archived ... archived on PubMed Central yours — you keep the copyright Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp BioMedcentral Journal of Foot and Ankle Resea...

Ngày tải lên: 10/08/2014, 21:23

... http://jmedicalcasereports.com/jmedicalcasereports/article/view/3/3/6505 Do you have a case to share? Submit your case report today • Rapid peer review • Fast publication • PubMed indexing • Inclusion in Cases Database Any patient, any case, can teach us something www.casesnetwork.com After ... during pregnancy requires a degree of clinical suspicion and should be confirmed by imaging dia...

Ngày tải lên: 11/08/2014, 17:21

... sepsis. Competing interests In 2001 our research group received a grant from Eli-Lilly (Italy) to conduct an original study on factors influencing length of stay in critical patients with sepsis. In Italy, ... uncertain. In Italy, the use of ATIII is very common in intensive care units (ICUs). For this reason we undertook an observational study to determine the pattern of us...

Ngày tải lên: 12/08/2014, 19:21

... HIV-1 entry kinetics, and sensitivity to inhibition by neutralizing antibodiesFigure 8 The relationship between N362, HIV-1 entry kinetics, and sensitivity to inhibition by neutralizing antibodies. ... progress to AIDS. In addition to dictating HIV-1 coreceptor specificity, the Env glycoproteins cause significant cytotoxicity both in vitro and in vivo. Env mediates most of the a...

Ngày tải lên: 13/08/2014, 05:22

... shock analysis, budding index and flow cytometry analysis Heat shock analysis was performed by incubating cells at 55°C for 12 minutes and then plating them on solid rich growth medium (YPD). ... spore germination and the following (first) mitotic cell cycle. Septins were proposed to maintain cell polarity during the mitotic cell cycle. By specifying a boundary between cortical domains, se...

Ngày tải lên: 14/08/2014, 08:20