Báo cáo y học: " Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation" pdf

... Miller Virology Journal 2010, 7:360 http://www.virologyj.com/content/7/1/360 Page 2 of 22 REVIEW Open Access Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation ... polynucleotide kinase/3’ phos- phatase (PNK). This enzyme catalyzes both the phos- phorylation of 5’-hydroxyl polynucleotide termini and the hydrolysis of 3’ -ph...

Ngày tải lên: 11/08/2014, 21:21

... to L -citrulline (at pH 7.5 and 37.0 °C) and of the trypsin catalyzed hydrolysis of N-a-benzoyl- L -arginine p-nitroanilide (at pH 6.8 and 21.0 °C) by N-amidino-2-hydroxypyrrolidine were deter- mined ... · 10 )1 M phosphate buffer) and 25.0 °C (Fig. 1). Values of k cat and K 0 m are independent of the enzyme assay. Biosynthesis of N -amidino-2-hydroxypyrrolidine N-Amidin...

Ngày tải lên: 17/03/2014, 23:20

... stimu- lated samples and the control. Zymography Protein levels of MMPs were assayed in conditioned media by gelatin and casein zymography. Equal volumes of medium samples and loading buffer (2 ... Puri- fication and cloning of aggrecanase-1: a member of the ADAMTS family of proteins. Science 1999, 284:1664-1666. 14. Sandy JD, Neame PJ, Boynton RE, Flannery CR: Catabolis...

Ngày tải lên: 09/08/2014, 14:22

... homology analysis epoxide hydrolase was classified as a member of a super family of hydrolytic enzymes including esterases and lipases, united by an a/b hydrolase fold and a similar catalytic triad ... applications. Polymers of epoxy and hydroxy fatty acids are important constituents of the cutin layer and serve as a protective barrier against stress [16]. Certain epoxy and...

Ngày tải lên: 31/03/2014, 08:20

... was performed by immunofluo- rescence and immunohistochemistry. Immunofluorescence detection Disclosure of secondary antibody binding was performed by incubation in 1:50 dilution of rhodamine-conjugated ... display any positive staining (Figure 2b). Table 1 Details of the immunohistochemistry methodology employed Target Antigen retrieval Blocking step and primary antibody Second...

Ngày tải lên: 09/08/2014, 01:22

... anti- body. We are grateful to Professor Mike Salmon and Professor Chris Buckley for critical reading of the manuscript. We acknowledge the financial support of the Henry Smith’s Charity and the ... majority of synovial fluid T cells from patients with JIA are of the memory phenotype [2]. Memory T cells have an increased capacity to bind to inflamed endothelium because of increa...

Ngày tải lên: 09/08/2014, 01:23

... diabetes mellitus, hyperlipidemia, and hypertension, and she t ook prednisolone (15 mg/day) and Dapsone (75 mg/day for seven days and 100 mg/ day for about six weeks) for LABD. Her physical exami- nation, ... because of septic shock caused by an anal fistula. She died of sepsis on hospital day 80. Conclusion: One of the side effects of Dapsone is agranulocytosis. Patients wi...

Ngày tải lên: 11/08/2014, 00:23

... minimum of one year. The blood levels of doxycy- cline and hydroxychloroquine were measured every other mont h and doses adapted to therapeutic levels (doxycy- cline: > 5 μg/ml, hydroxychloroquine ... association of a lysotropic agent (hydroxychloroquine) to doxycycline tends to reduce the acidity of the vacuole in which Tropheryma whipplei is located and thus improves the ef...

Ngày tải lên: 11/08/2014, 07:20

... Since these patients usually have early exfoliat ion of the teeth and, conse- quently, maxillomandibular bony atrophy, treatment with a combination of onlay bone grafting and a sinus lift technique ... hereditary disturbance of dentin formation characterized by defective dentin development with clinically normal appearing crowns, severe hypermobility of teeth and spontaneous de...

Ngày tải lên: 11/08/2014, 14:21

... anti- gens of ORF 73 ,ORF 65 and K8.1 using enzyme-linked immunosorbent assays as described 22,24 Error! Reference source not found. This assay has a sensitivity of 82% and a specificity of 96% 22 . ... infection by seroprevalence and high antibody titers in different ethnic groups Wang et al. Virology Journal 2010, 7:62 http://www.virologyj.com/content/7/1/62 Page 5 of 7 A st...

Ngày tải lên: 12/08/2014, 04:20