Báo cáo y học: " Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation" pdf
Báo cáo y học: " Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation" pdf
... Miller Virology Journal 2010, 7:360http://www.virologyj.com/content/7/1/360Page 2 of 22REVIEW Open Access Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation ... polynucleotide kinase/3’ phos-phatase (PNK). This enzyme catalyzes both the phos-phorylation of 5’-hydroxyl polynucleotide termini and the hydrolysis of 3’ -phosphomonoesters and 2’ :3’-cycl ... restriction by way of the phage-inducedpolynucleotide kinase - 3’ phosphatase (pnk gene), whichconverts the tRNA 5’-hy droxyl and 3’-phosphate terminileft by PrrC, into 5’-phosphate and 3’-hydroxyl...
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Báo cáo Y học: Agmatine oxidation by copper amine oxidase Biosynthesis and biochemical characterization of N-amidino-2-hydroxypyrrolidine pdf
... toL-citrulline (at pH 7.5 and 37.0 °C) and of the trypsin catalyzed hydrolysis of N-a-benzoyl-L-arginine p-nitroanilide (at pH 6.8 and 21.0 °C) by N-amidino-2-hydroxypyrrolidine were deter-mined ... ·10)1Mphosphate buffer) and 25.0 °C (Fig. 1). Values of kcat and K0mare independent of the enzyme assay.Biosynthesis of N-amidino-2-hydroxypyrrolidineN-Amidino-2-hydroxypyrrolidine was synthesized ... N-amidino-2-hydroxypyrrolidine is hosted in the hydrophobic cavitydefined by the heme p rosthetic group and by the facinghydrophobic residues Ala270 and Val271, as observed for anumber of nitrogen...
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Báo cáo y học: "Tumor necrosis factor alpha-dependent aggrecan cleavage and release of glycosaminoglycans in the meniscus is mediated by nitrous oxide-independent aggrecanase activity in vitro" pptx
... stimu-lated samples and the control. ZymographyProtein levels of MMPs were assayed in conditioned media by gelatin and casein zymography. Equal volumes of mediumsamples and loading buffer (2 ... Puri-fication and cloning of aggrecanase-1: a member of theADAMTS family of proteins. Science 1999, 284:1664-1666.14. Sandy JD, Neame PJ, Boynton RE, Flannery CR: Catabolism of aggrecan in ... catabolism is initiated by proteoglycan degradationfollowed by that of collagen fibers. Therefore, our studyfocused on the TNFα-dependent depletion of proteoglycansin a three-day bovine in vitro...
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Báo cáo Y học: Brassica napus soluble epoxide hydrolase (BNSEH1) Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris docx
... homology analysis epoxide hydrolasewas classified as a member of a super family of hydrolyticenzymes including esterases and lipases, united by an a/bhydrolase fold and a similar catalytic triad ... applications.Polymers of epoxy and hydroxy fatty acids are importantconstituents of the cutin layer and serve as a protectivebarrier against stress [16]. Certain epoxy and hydroxy fattyacids have ... carboxylate modifying agents such asN,N¢-dicyclohexylcarbodiimide and its hydrolysis product,N,N¢-dicyclohexylurea, showed strong inhibition of mam-malian sEH using 4-nitrophenyl-trans-2,3-epoxy-3-phenyl-propyl...
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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot
... was performed by immunofluo-rescence and immunohistochemistry.Immunofluorescence detectionDisclosure of secondary antibody binding was performed by incubation in 1:50 dilution of rhodamine-conjugated ... display any positive staining(Figure 2b).Table 1Details of the immunohistochemistry methodology employedTarget Antigen retrieval Blocking step and primary antibody Secondary antibodyAggrecan ... polyA -mRNA was performed as anassessment of cell metabolic activity as described previously[39,40].Immunofluorescence and immunohistochemistryImmunofluorescence and immunohistochemistry were...
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Báo cáo y học: " An association between the acute phase response and patterns of antigen induced T cell proliferation in juvenile idiopathic arthritis" ppt
... anti-body. We are grateful to Professor Mike Salmon and Professor ChrisBuckley for critical reading of the manuscript. We acknowledge thefinancial support of the Henry Smith’s Charity and the ... majority of synovial fluid T cells from patients with JIAare of the memory phenotype [2]. Memory T cells have anincreased capacity to bind to inflamed endotheliumbecause of increased expression of ... neutrophils,monocytes and lymphocytes, and it is possible that theaccumulation of activated CD14+monocytes may con-tribute to the different proliferative patterns seen [17–19].Also, JIA patients with systemic...
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Báo cáo y học: " Dapsone-induced agranulocytosis leading to perianal abscess and death: a case report" pdf
... diabetes mellitus, hyperlipidemia, and hypertension, and she t ook prednisolone (15 mg/day) and Dapsone (75 mg/day for seven days and 100 mg/day for about six weeks) for LABD. Her physical exami-nation, ... because of septic shock caused by an anal fistula. She died of sepsis on hospital day 80.Conclusion: One of the side effects of Dapsone is agranulocytosis. Patients with agranulocytosis may be ... reintubation and initiation of mechanical ventilation and tracheostomywas performed on Day 15. Postsacral erosion and induration appeared on Day 18. On the same day, herbody temperature was >39°C and...
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Báo cáo y học: " Tropheryma whipplei tricuspid endocarditis: a case report and review of the literature" ppsx
... minimum of one year. The blood levels of doxycy-cline and hydroxychloroquine were measured every othermont h and doses adapted to therapeutic levels (doxycy-cline: > 5 μg/ml, hydroxychloroquine ... association of a lysotropic agent(hydroxychloroquine) to doxycycline tends to reduce theacidity of the vacuole in which Tropheryma whipplei islocated and thus improves the efficacy of doxycyclineinactive ... our patient was initially treated by ceftriaxone then with a combination of sulfamethoxa-zole/trimethoprim, hydroxychloroquine and doxycyclinefor one year. By analogy with what is known about...
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Báo cáo y học: "Dentin dysplasia type I: a case report and review of the literature" ppsx
... Since these patientsusually have early exfoliat ion of the teeth and, conse-quently, maxillomandibular bony atrophy, treatmentwith a combination of onlay bone grafting and a sinuslift technique ... hereditary disturbance of dentin formation characterized by defectivedentin development with clinically normal appearing crowns, severe hypermobility of teeth and spontaneousdental abscesses or cysts. ... successfully [15].ConclusionDentin dysplasia type I is a rare inherited abnormality of the dentin that leads to premature exfoliation of the pri-mary and permanent teeth. Early diagnosis of the...
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Báo cáo y học: " Human herpesvirus-8 in northwestern China: epidemiology and characterization among blood donors" pdf
... anti-gens of ORF73,ORF65 and K8.1 using enzyme-linkedimmunosorbent assays as described22,24Error! Referencesource not found. This assay has a sensitivity of 82% and a specificity of 96%22. ... infection by seroprevalence and high antibody titers in different ethnic groupsWang et al. Virology Journal 2010, 7:62http://www.virologyj.com/content/7/1/62Page 5 of 7A strong association of HHV-8 ... levels of HHV-8 antibody titersamong HHV-8-positive subjects. High titers were notedin: 12.2% of those aged <30 years, 13.4% of sub jectsaged >30 years, 12% of unmarried individuals, and 13.9%...
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