Báo cáo y học: " Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx

... helicase assembles by surrounding the lagging strand and excluding the leading strand of DNA. ATP hydrolysis translocates the enzyme 5’ to 3’ along the lag - ging DNA strand, thereby unwinding the ... domain of DnaC is an AAA + ATPase related to DnaA, as revealed by the structure of a truncated DnaC from Aquifex aeolicus (pdb 3ec2) [75]. The DnaC N-domain interacts...

Ngày tải lên: 11/08/2014, 21:21

... Two alternative mechanisms for initiation of DNA replication forks in bacteriophage T4: Priming by RNA polymerase and by recombination. Proceedings of the National Academy of Sciences of the United ... Initiation of bacte riophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and...

Ngày tải lên: 11/08/2014, 21:21

... containing protease inhibitor cocktail and lysed by sonication. After centrifugation, the supernatant was loaded onto a DEAE- Sepharose column, and eluant containing His-tagged yeast TDP was then ... replication, repair and transcrip- tion processes by making single stranded breaks on DNA, unwinding and religating the DNA ends in the cleaved strand [1]. During the pr...

Ngày tải lên: 31/03/2014, 21:21

... Germany) in both linear and reflection TOF configurations at an acceleration voltage of 20 kV and delayed ion extraction. The samples were dissolved in aqueous triethylamine (0.07 M )ata concentration ... that the LPS prepared by standard phenol–water extraction was heavily contaminated with an amylopectin-like glucan, a a-(1–6)-linear glucan, and a short chain b-(1–6)-gluca...

Ngày tải lên: 08/03/2014, 09:20

... 2002 accordance with the common acylation pattern of E. coli lipid A [18]. Analytical HPAEC revealed that the deacylated LPS fraction contained one major oligosaccharide isolated by semipreparative ... alkaline deacylation one major oligosaccharide by high-performance anion-exchange chro- matography (HPAEC) which had a molecular mass of 2486.59 Da as determined by electrospray ion...

Ngày tải lên: 08/03/2014, 09:20

... UK; 3 Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada Structural analysis of the lipopolysaccharide (LPS) of nontypeable Haemophilus in uenzae strain ... unencapsulated (nontypeable) forms. Nontypeable H. in uenzae (NTHi) strains commonly colonize the nasopharynx of healthy carriers and are important causes of upper and l...

Ngày tải lên: 31/03/2014, 21:21

... RT-PCR analysis reveals increased levels of mRNA expression of BAFF and APRIL in aged thymocytes. BAFF and APRIL are both involved in maturation and survival of B cells, and their expression levels ... by a general decline in the ability to resist infection and an increase in autoimmune complications such as type 2 diabetes, inflammation, and cancer [1-9]. O...

Ngày tải lên: 03/11/2012, 11:35

... Impressions Rating Scale in antidepressant clinical trials: a replication analysis. Int Clin Psychopharmacol 2004, 19:157-160. 7. American Psychiatric Association: Diagnostic and Statistical Manual of Mental ... sensitivity of the Montgomery-sberg Depression Rating Scale, the Hamil- ton Depression Rating Scale and the Clinical Global Impres- sions Rating Scale in antidepr...

Ngày tải lên: 08/08/2014, 23:21

... analysis was performed by DAQ. RNA and DNA extraction was performed by IK, KKL, and MDT. JPL contributed mice and tumor samples and KKL performed array analysis for the confirmation study. Taqman ... for which a measurement was available. Statistical analysis was performed with the R package [32]. Statistical analysis of gene expression Permutation analysis of hi...

Ngày tải lên: 09/08/2014, 22:23

... participated in the design and coordination of the study. LD provided reagents and participated in the design and coordination of the study, as well as in the analysis of the data and drafting and ... including Gabon, India, Indone- sia, Italy, Malaysia, and Sri Lanka, in 2007, and Singa- pore and Sri Lanka in 2008. Most of these outbreaks were...

Ngày tải lên: 12/08/2014, 04:21