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Báo cáo y học: " Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx

Báo cáo y học:

Báo cáo y học: " Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx

... helicase assembles by surrounding the laggingstrand and excluding the leading strand of DNA. ATPhydrolysis translocates the enzyme 5’ to 3’ along the lag -ging DNA strand, thereby unwinding the ... domain of DnaC is anAAA + ATPase related to DnaA, as revealed by the structure of a truncated DnaC from Aquifex aeolicus(pdb 3ec2) [75]. The DnaC N-domain interacts with the hexameric DnaB in a one-to-one ... promotes DNA unwinding and the sub-sequent bi-directional loading of DnaB, the replicativehelicase [5]. Assembly of the replication complex and synthesis of an RNA primer by DnaG initiates the synthesis...
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Báo cáo y học:

Báo cáo y học: " Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx

... Two alternative mechanisms for initiation of DNA replication forks in bacteriophage T4: Priming by RNA polymerase and by recombination. Proceedings of the National Academy of Sciences of the United ... Initiation of bacte riophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives. Virology Journal 2010 7:358.Submit your ... of Kadyrov and Drake [98]. The in vitro strand switching analyzed by Kadyrov and Drake had severa l properties that resembl e the repl ica-tion of damaged DNA during T4 infections. Certainalleles...
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Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot

Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot

... containingprotease inhibitor cocktail and lysed by sonication. Aftercentrifugation, the supernatant was loaded onto a DEAE-Sepharose column, and eluant containing His-tagged yeastTDP was then ... replication, repair and transcrip-tion processes by making single stranded breaks on DNA, unwinding and religating the DNA ends in the cleavedstrand [1]. During the process, DNA becomes covalentlylinked ... encodes a His tag and a thrombin cleavage site at the N-terminus of yeast TDP and was confirmed by restriction enzymemapping and by nucleotide sequencing. The yeast TDPcoding sequence obtained in...
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Báo cáo Y học: Structural analysis of Francisella tularensis lipopolysaccharide potx

Báo cáo Y học: Structural analysis of Francisella tularensis lipopolysaccharide potx

... Germany) in bothlinear and reflection TOF configurations at an accelerationvoltage of 20 kV and delayed ion extraction. The sampleswere dissolved in aqueous triethylamine (0.07M)ataconcentration ... that the LPS prepared by standardphenol–water extraction was heavily contaminated with anamylopectin-like glucan, a a-(1–6)-linear glucan, and a shortchain b-(1–6)-glucan. b-(1–6)-Glucans are ... core;lipid A. Francisella tularensis is a Gram-negative bacterium whichcauses tularemia, a severe and often fatal disease of humans and other mammals [1]. The bacterium is an intracellularpathogen and...
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Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot

Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot

... 2002accordance with the common acylation pattern of E. colilipid A [18].Analytical HPAEC revealed that the deacylated LPSfraction contained one major oligosaccharide isolated bysemipreparative ... alkaline deacylation one majoroligosaccharide by high-performance anion-exchange chro-matography (HPAEC) which had a molecular mass of 2486.59 Da as determined by electrospray ionization massspectrometry. ... structural characterization of epitopesleading to cross-reactivity, the full assignment of carbon and, in particular, proton resonances now provides the basis for detailed NMR-based conformational analysis...
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Báo cáo Y học: Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003 potx

Báo cáo Y học: Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003 potx

... UK;3Institute for Biological Sciences,National Research Council of Canada, Ottawa, Ontario, Canada Structural analysis of the lipopolysaccharide (LPS) of nontypeable Haemophilus in uenzae strain ... unencapsulated (nontypeable) forms. NontypeableH. in uenzae (NTHi) strains commonly colonize the nasopharynx of healthy carriers and are important causes of upper and lower respiratory tract infections [1]. ... Hex3 glycoforms lacking acetylgroups or containing a second glycyl group were alsoindicated (data not shown). I n addition to the m ajorHex2 and Hex3 glycoforms, trace amounts of Hex1 and Hex4...
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Báo cáo y học:

Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"

... RT-PCR analysis reveals increased levels of mRNA expression of BAFF and APRIL in aged thymocytes. BAFF and APRIL are both involved in maturation and survival of B cells, and their expression levels ... by a general decline in the ability to resist infection and an increase in autoimmune complications such as type 2 diabetes, inflammation, and cancer [1-9]. One of the underlying causes of ... Pathway analysis (www.ingenuity.com) identi-fied signaling pathways and gene families that were affected in aging thymocytes. Table 2A lists all af-fected functions regardless of age and Table...
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Báo cáo y học: "An analysis of correlations among four outcome scales employed in clinical trials of patients with major depressive disorder" pdf

... ImpressionsRating Scale in antidepressant clinical trials: a replication analysis. Int Clin Psychopharmacol 2004, 19:157-160.7. American Psychiatric Association: Diagnostic and Statistical Manual of Mental ... sensitivity of the Montgomery-sberg Depression Rating Scale, the Hamil-ton Depression Rating Scale and the Clinical Global Impres-sions Rating Scale in antidepressant clinical trials. Int ClinPsychopharmacol ... Washington, DC, USA: American Psychi-atric Association; 1987. 9. American Psychiatric Association: Diagnostic and Statistical Manual of Mental Disorders (DSM-IV) Washington, DC, USA: American...
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Báo cáo y học: ": Network analysis of skin tumor progression identifies a rewired genetic architecture affecting inflammation and tumor susceptibility" docx

... analysis wasperformed by DAQ. RNA and DNA extraction was performed by IK, KKL, and MDT. JPL contributed mice and tumor samples and KKL performed array analysis for the confirmation study. Taqman ... forwhich a measurement was available. Statistical analysis was performed with the R package [32].Statistical analysis of gene expressionPermutation analysis of hierarchical clustering was per-formed ... ted only in carcino-mas, in 45 cases the transcript was expressed only in carcinomas and not in normal tissue. This may be dueto activation of signaling pathways not expressed in normal skin,...
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Báo cáo y học: " Genetic analysis of chikungunya viruses imported to mainland China in 2008" pps

... participated in the design and coordination of the study. LD provided reagents and participated in the design and coordination of the study, as well as in the analysis of the data and drafting and ... including Gabon, India, Indone-sia, Italy, Malaysia, and Sri Lanka, in 2007, and Singa-pore and Sri Lanka in 2008. Most of these outbreakswere attributed to variants of the central/east Africangenotype ... infec-tions originated in Sri Lanka and Malaysia, respec-tively, rather than in China, as the disease has anincubation period of about three to twelve days [2] and no other local close contacts...
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