Báo cáo y học: " Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
Báo cáo y học: " Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
... this article as: Kreuzer and Brister: Initiation of bacte riophage T4
DNA replication and replication fork dynamics: a review in the Virology
Journal series on bacteriophage T4 and its relatives. ... Two alternative mechanisms for initiation of DNA
replication forks in bacteriophage T4: Priming by RNA polymerase and
by recombination....
Báo cáo y học: " Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
... helicase assembles by surrounding the lagging
strand and excluding the leading strand of DNA. ATP
hydrolysis translocates the enzyme 5’ to 3’ along the lag -
ging DNA strand, thereby unwinding the ... promotes DNA unwinding and the sub-
sequent bi-directional loading of DnaB, the replicative
helicase [5]. Assembly of the replication complex and
synthesis of...
Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot
... absorptivity of e
1
and e
and approximately the same slope of the k
1
and k (Fig. 3)
suggests that thenatureofthefirstkineticphaseofthereaction
between Yb
3+
and apo-Tf is the same as that of the reaction
between ... blood plasma with a concentration of
35 l
M
.Itisonly30%saturatedwithironinbloodplasma
and has the capacity for binding to other metal ions of
ther...
Báo cáo Y học: Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes A surface plasmon resonance study pdf
... faster
than for the Gla-domain presumably due to a further
stabilization of the structure of the Gla-domain, indicating
that less than 1% of the free isolated Gla-domain has a
conformation that is appropriate ... membranes containing 25 mol% PtdSer in the presence of
10 m
M
Ca
2+
obtained by evaluation of association and dissociation phases (I) and equilibrium bi...
Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt
... DNA replication assay. In both the
SV40 and JCV assays, incorporation of nucleotides is
dependent upon an intact origin of replication; either
plasmids carrying the noncognate PyV origin or a disabled
JCV ... with
human and murine DNA polymerase a- primase, respectively; columns
4 and 5, JCV Tag with human and murine DNA polymerase a- pri-
mase, respectively. D...
Báo cáo Y học: Regulation of glypican-1, syndecan-1 and syndecan-4 mRNAs expression by follicle-stimulating hormone, cAMP increase and calcium influx during rat Sertoli cell development pptx
... 5¢-CCTTTGAGCACATTTCGGCAA-3¢
for glypican-1; 5¢-ACAGACTACCTCATGAAGAT-3¢
and 5¢-AGCCATGCCAAATGTCTCAT-3¢ for b-actin.
The PCR was started at 94 °Cfor1minandfollowedby
up to 27 cycles of amplification for ... were 5¢-AGGT
GCTTTGCCAGATATGACT-3¢ and 5¢-CTCTTTGATG
ACAGAAGTGCCT-3¢ for syndecan-1; 5¢-GAGTCGAT
TCGAGAGACTGA-3¢ and 5¢-AAAAATGTTGCTGCC
CTG-3¢ for syndecan-4; 5¢-GAATGACTCGGAGCGTAC...
Báo cáo Y học: Synthesis of phosphoenol pyruvate (PEP) analogues and evaluation as inhibitors of PEP-utilizing enzymes pot
... modifica-
tions. Among the carboxylate-modified analogues, only
those replaced by a negatively charged group inhibited
pyruvate kinase and enolase. Remarkably, the activity of
PEP carboxylase was ... allowed the isolation and characterization of stable
fluoro analogues of the otherwise unstable tetrahedral
intermediate of the normal reaction [1].
The carboxylic and t...
Báo cáo Y học: Structure of human immunodeficiency virus type 1 Vpr(34–51) peptide in micelle containing aqueous solution pptx
... calculation was performed using
XPLOR
3.851 and a modified ab initio simulated annealing
protocol including floating assignment of prochiral groups
and a conformational database potential during ... & Srinivasan, A. (1995) Muta-
genesis of the putative alpha-helical domain of the Vpr protein of
human immunodeficiency virus type 1: effect on stability and
virion incorp...
Báo cáo Y học: Expression of glucose transporter-2, glucokinase and mitochondrial glycerolphosphate dehydrogenase in pancreatic islets during rat ontogenesis potx
... frozen at )80 °C until the
insulin assays. Insulin was measured by the radioimmun o-
assay method of Herbert et al. [15], using rat insulin as a
standa rd.
RNA isolation and Northern-blot analysis
Total ... subtracted the change in A
412
before the
addition of oxalacetate (which is acetyl-CoA hydrolase
activity) from the change in A
412
after addition of oxalace-
t...
Báo cáo Y học: Comparison of starch branching enzyme I and II from potato ppt
... in the iodine-activity assay
with commercially available amylose and amylopectin as
substrates. The delta absorbance at 655 nm for the amylose
substrate and 520 nm for the amylopectin substrate, ... min and analysed by the starch –iodine assay.
Incubation of linear dextrins with branching enzyme for
gel-permeation chromatography (GPC) and
high-performance anion-exchange...
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