... 7:13http://www.head-face-med.com/content/7/1/13Page 3 of 9RESEARC H Open Access Effects of enamel matrix derivative and transforming growth factor-b1 on human osteoblastic cellsDaniela B Palioto1*, Thaisângela ... the effects of enamel matrix derivative (EMD), TGF-b1, and thecombination of both factors (EMD+TGF-b1) on human osteoblastic cell cultures.Methods: Cells were obtained from alveolar bone of ... digestion. Effects of EMD, TGF-b1, or a combination of both were analyzed on cell proliferation, bone sialoprotein (BSP), osteopontin(OPN) and alkaline phosphatase (ALP) immunodetection, total...