... generously donated byB. Thorens, Lausanne, Switzerland, using the DIGRNA labelling kit (Boehringer-Mannheim, Germany).CDNA synthesis, PCR ampli®cations, and Southern-blotanalysis Using random ... s the metabolism o f glucose in these cells and then the synthesis and secretion of insulin. In contrast with these ev ents, fetal pancreatic b cells secreteinsulin poorly in response to increased ... 2.8% and 7.8% of the total content in 5 and 10-day-old rats, respectively, compared with the 14.7%found in young adult animals.Ontogenesis ofGLUT-2 and glucokinase mRNA and protein in rat pancretic...