... B56, and PR72-type Bsubunits in organisms as diverse as Neurospora crassa,Candida tropicalis, Dictyostelium discoideum, Medicagovaria (alfalfa), Arabidopsis t haliana, Oryza sativa (rice),Caenorhabditis ... R., Yang, F., Nakamura, M.,Fujisawa, H., Kleihues, P. & Walter, G. (2001) Reduced expres-sion of the Aalpha subunit of protein phosphatase 2A in humangliomas in the absenc e of mutations ... [buffer A containing 0.1% nonidetp40 (NP-40) and 0.25% BSA] and 20 lLofapre-washed 1 : 1 slurry of glutathione±Sepharose (AmershamPharmacia) was added. Incubation continued f or 2 h at4 °C...