... ATG TAC CC-3' (sense) and 5'-GAC ATC TATCAG TGG TCG TG-3' (antisense), and 5'-AAT GGG AGGAGC TTC CAG AG-3' (sense) and 5'-ACC AAC CCC ATATTC AGC AG-3' (antisense). ... phos-phatase activity and osteocalcin release. Alkaline phosphataseactivity was determined on cell aliquots by substrate hydrolysiswith p-nitrophenyl phosphate, and osteocalcin release wasdetermined ... system's manual (Pierce, Brockville, Ontario, Can-ada). COX-2 levels were determined with a polyclonal rabbitanti-human COX-2 antibody (Cayman Chemical-Cedarlane,Hornby, Ontario, Canada) at a 1:400...