Enzymes in the Environment: Activity, Ecology and Applications - Chapter 21 (end) pps
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 21 (end) pps
... be- of 7-amino-4-meth- cause they have ylcoumarin. high k cat /k m values and low back- Prepare proteins with ground hydrolysis a fluorogenic label and the thiol leav- attached to individ- ing ... hydro- lyzes neutral amino acid arylamides: i.e., it hydrolyzes amino acids attached to β-naph- thylamine and p-nitroaniline according to the following reaction (using the amino acid l- l...
Ngày tải lên: 11/08/2014, 15:20
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 1 ppsx
... systems involving phenoloxidase enzymes. The deamination of amino acids, such as serine, phenylalanine, proline, methionine, and cysteine by birnessite, and the role of pyrogallol in influencing their ... effective for both L- and D- glutamic acid. The PLP-Cu 2ϩ -smectite has acted as a ‘‘pseudoenzyme’’ wherein the PLP was active and independent of the protein matrix of t...
Ngày tải lên: 11/08/2014, 15:20
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 2 pptx
... Inc. Currently,itisevidentthatmicroorganismsformcomplexmicrobialfoodwebsin allaquaticecosystems,andthattheiractivitiesandmetabolismsoftenaretightlycoupled and/ ormutuallyaffected(132,143,144).Therefore,itisnotsurprisingthatenzymatic propertiesandactivitiesofdifferentcomponentscreatingthemicrobialfoodwebsinlake ecosystemshavedemonstratedcloserelationships.Severalreportshavedocumentedthe stron...
Ngày tải lên: 11/08/2014, 15:20
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 3 pdf
... for chitin-hydrolyzing activity by using MUF-β-d-N, N′-diacetylchitobioside, and chitobiase activity was then assayed in protein extracts prepared from the positive clones. The chi- tinases of marine bacteria ... Inc. Investigationsofextracellularenzymesfrommarineanimalsandenzymesisolatedfrom prokaryotesareconsideredonlyifaclearconnectiontomarineecologyisestablished. Thetermextracellu...
Ngày tải lên: 11/08/2014, 15:20
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 4 potx
... Inc. Althoughthisstudyinvolvedtheuseofageneticallymodifiedmicrobe,themodi - cationswerenotintendedtohaveafunctionalimpact;theywereinsertedasgeneticmark- ers.Asecondstudycomparingtheeffectofthesamegeneticallymarkedstraintothatof afunctionallymodifiedstrainshowedeffectsthataremoreinteresting(36).Theaimof thisworkwastodeterminetheimpactintherhizosphereofwildtypealongwithfunction- allyandnonfunc...
Ngày tải lên: 11/08/2014, 15:20
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 5 ppt
... short-chain poly-P was higher in the internal hyphae (67). Long-chain poly-P seems to be more efficient in transporting Pi from the extraradical to the intraradical part of the fungi. Activity of enzymes ... Inc. directlycontributetoreductionofpathogenviabilityandgrowth.Inaddition,theyhave beenproposedasmediatorsinpathwaysleadingtodefense-relatedgeneexpression(136). ThereleaseofA...
Ngày tải lên: 11/08/2014, 15:20
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 8 potx
... soil enzymes, laccase and tyrosinase. The potential role of these enzymes in the humification of anilinic and phenolic compounds and reduction of their bioavilability with the passage of time (aging) ... affecting the efficiency of interaction of the substrate and enzyme molecules. In other words, a portion of the enzyme molecules existing in the field soil may not...
Ngày tải lên: 11/08/2014, 15:20
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 9 potx
... each site and placed in litter bags. In using the ap- proach of Sinsabaugh et al. (73) and Jackson et al. (29), confined and in situ POM samples were assayed monthly for β-glucosidase, β-N-acetylglucosaminidase, ... Inc. wererepressedbyaddedN;formapleandoak,theseactivitiesincreased.Theresults suggestedthatwhiterotfungi,whichproduceligninasesinresponsetolowNavailability, weredispl...
Ngày tải lên: 11/08/2014, 15:20
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 10 docx
... the years; these include vanilin, indulin, ferrulic acid, and, most importantly, 14 C-labeled synthetic lignins. Various fungal enzymes are involved in lignin degradation, including lignin peroxidase, ... strains and the extrac- tion of enzymes, provide complementary information on enzyme production by emphasi- zing the potential of the living hyphae and the sum of past a...
Ngày tải lên: 11/08/2014, 15:20
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 11 ppsx
... Inc. possibletofindseveralexplanationstointerpretaproteinadsorptionisotherm,withno experimentalevidenceavailabletochooseamongthem.TheadvantageoftheNMR methodisthatitsimultaneouslygivesthequantityofadsorbedprotein,thesurfacecover- ageofthesolidbytheprotein,andthemonolayerormultilayermodeofadsorption(16). Onlyknowledgeofthesethreefactorsallowsapossibleunfoldingoftheproteinsonthe claysurfacestobe...
Ngày tải lên: 11/08/2014, 15:20