Enzymes in the Environment: Activity, Ecology and Applications - Chapter 15 pps

... Inc. assuchanalysesassumesampleindependence.Thisinformationisvaluableinthedesign ofsamplingstrategiesforbioindicatorsasanunderstandingoftherepresentativenessof samplesofalargerareaiscritical.Thethirdimportantfeatureisthenugget.Theoretically, whenthelagdistanceiszero(samplestakenatthesamepoint)thereshouldbenovariance. OftenthesemivariograminterceptsalongtheYaxis,notattheorigin,andthisisknown a...

Ngày tải lên: 11/08/2014, 15:20

... aspartase-Ca-montmoril- lonite systems (159 ). Deamination of l- and d-glutamic and aspartic amino acids and of their DL racemic mixtures in the presence of Na-montmorillonite showed a stereoselectiv- ity ... effective for both L- and D- glutamic acid. The PLP-Cu 2ϩ -smectite has acted as a ‘‘pseudoenzyme’’ wherein the PLP was active and independent of the protein matrix o...

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... Inc. possibletofindseveralexplanationstointerpretaproteinadsorptionisotherm,withno experimentalevidenceavailabletochooseamongthem.TheadvantageoftheNMR methodisthatitsimultaneouslygivesthequantityofadsorbedprotein,thesurfacecover- ageofthesolidbytheprotein,andthemonolayerormultilayermodeofadsorption(16). Onlyknowledgeofthesethreefactorsallowsapossibleunfoldingoftheproteinsonthe claysurfacestobe...

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... systems, lasR-lasI and rhlR-rhlI. The lasI and rhlI gene products are involved in the synthesis of two different AHL molecules, N-(3-oxododecanoyl)-l- homoserine lactone and N-buytryl-l-homoserine lactone, ... components in- clude β-galactosidase, β-N-acetylglucosaminidase, β-N-acetylgalactosaminidase, - and β-mannosidase, and α-fucosidase (116). Other bacteria then produ...

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... Inc. Currently,itisevidentthatmicroorganismsformcomplexmicrobialfoodwebsin allaquaticecosystems,andthattheiractivitiesandmetabolismsoftenaretightlycoupled and/ ormutuallyaffected(132,143,144).Therefore,itisnotsurprisingthatenzymatic propertiesandactivitiesofdifferentcomponentscreatingthemicrobialfoodwebsinlake ecosystemshavedemonstratedcloserelationships.Severalreportshavedocumentedthe stron...

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... for chitin-hydrolyzing activity by using MUF-β-d-N, N′-diacetylchitobioside, and chitobiase activity was then assayed in protein extracts prepared from the positive clones. The chi- tinases of marine bacteria ... Inc. Investigationsofextracellularenzymesfrommarineanimalsandenzymesisolatedfrom prokaryotesareconsideredonlyifaclearconnectiontomarineecologyisestablished. Thetermextracellu...

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... Inc. Althoughthisstudyinvolvedtheuseofageneticallymodifiedmicrobe,themodi - cationswerenotintendedtohaveafunctionalimpact;theywereinsertedasgeneticmark- ers.Asecondstudycomparingtheeffectofthesamegeneticallymarkedstraintothatof afunctionallymodifiedstrainshowedeffectsthataremoreinteresting(36).Theaimof thisworkwastodeterminetheimpactintherhizosphereofwildtypealongwithfunction- allyandnonfunc...

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... short-chain poly-P was higher in the internal hyphae (67). Long-chain poly-P seems to be more efficient in transporting Pi from the extraradical to the intraradical part of the fungi. Activity of enzymes ... drought on non- mycorrhizal and mycorrhizal maize: Changes in the pools of non-structural carbohydrates, in the activities of invertase and trehalase, and in...

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... soil enzymes, laccase and tyrosinase. The potential role of these enzymes in the humification of anilinic and phenolic compounds and reduction of their bioavilability with the passage of time (aging) ... Inc. (nitrificationanddenitrificationeffects)aswellasbyprotectionandcreationofwetlands (4,7,39,57,85,122). Itisagainstthisbackdropofthemajorenvironmentalrelevanceoftheenzymesof...

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... each site and placed in litter bags. In using the ap- proach of Sinsabaugh et al. (73) and Jackson et al. (29), confined and in situ POM samples were assayed monthly for β-glucosidase, β-N-acetylglucosaminidase, ... Inc. wererepressedbyaddedN;formapleandoak,theseactivitiesincreased.Theresults suggestedthatwhiterotfungi,whichproduceligninasesinresponsetolowNavailability, weredispl...

Ngày tải lên: 11/08/2014, 15:20