báo cáo khoa học: " The increasing chronicity of HIV in sub-Saharan Africa: Re-thinking “HIV as a long-wave event” in the era of widespread access to ART" pot

Báo cáo khoa học:Chính sách đối ngoại của Đảng với khu vực Đông Nam Á những năm đầu thế kỷ 21 pot

... DANG LANH OAO CONG CUOC DOI MOI CHINH SACH DOI NGOAI CUA DANG V6l KHO VlfC DONG NAM A NHfiNG NAM DAU THE KY XXI ThS NGUYEN THIHOAN Phien hgp todn the Diin ddn khu vuc ASEAN ... Bg trudng Ngoai giao cac nudc ASEAN ldn thfl 34 (AMM 34) diin ra tai Ha Ngi, Thfl tudng Phan Van Khai khang djnh: "Chinh sach ASEAN Id mgt bg phdn cdu thdnh quan trgng trong ... nudc ASEAN"'-'. Viec thflc day mdi quan he vdi cdc nudc trong khu vyc Ddng Nam A mgt each toan dien se gdp phan cflng co ldng tin cfla cdc nudc ASEAN ndi rieng va cac ddi tac...

Tài liệu Báo cáo khoa học: High afﬁnity copper binding by steﬁn B (cystatin B) and its role in the inhibition of amyloid ﬁbrillation docx

... versa were made in an enclosedreaction cell maintained at a constant temperature. The instrument measured the heat generated or absorbed as the ligand-macromolecule reaction occured. A binding ... protein, lacking the copper binding domain is cap-able of domain swapping and forms a dimer as revealedby crystal structure analysis [36]. In our case, SEC datacollected for samples at pH 7 have ... binding isothermwas ﬁtted to the data, expressed in terms of the heat changeper mole of ligand against the ligand to macromoleculeratio. From the binding isotherm values for the reactionstoichiometry,...

Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the ﬂounder Platichthys stellatus pptx

... protein as a novel l-amino acidoxidase (LAAO; EC.1.4.3.2). LAAOs catalyze the oxidative deamination of an l-amino acid substrateand have been reported to exert antibacterial activity in a variety ... antiserum against the antibacterial protein was obtainedby injecting a Japanese white rabbit (Kitayama Labes Co.Ltd., Nagano, Japan) with the insoluble recombinant pro-tein of His-tagged psLAAO puriﬁed ... 2B) andchromatofocusing (Fig. 2C). In gel ﬁltration chromato-graphy and chromatofocusing steps, the antibacterialactivity was eluted as a single peak. SDS ⁄ PAGE of the fractions containing antibacterial...

Báo cáo khoa học: An Escherichia coli twin-arginine signal peptide switches between helical and unstructured conformations depending on the hydrophobicity of the environment potx

... SufI, the 208 nmregion of the spectra indicates a slight increase in helicalcharacter for SufI-AA and a slight decrease for SufI-KK in TFE, whereas at 222 nm in water/TFE SufI-AA has lessintensity ... SufI. The twin-arginine translocation (Tat) system operates in the cytoplasmic membranes of most free-living bacteria and in the thylakoid membranes of plant chloroplasts [1–3].Operating alongside Sec-type ... periplasmic proteins that areexported only after binding any of a range of complexredox cofactors, such as molybdopterin or FeS centres[8–11]. These cofactors are inserted in the cytoplasm bycomplex...

báo cáo khoa học: "Implementing and managing self-management skills training within primary care organisations: a national survey of the expert patients programme within its pilot phase" pdf

... courses in the 2005–06 financial year, with a mean budget of £14,000assigned to EPP. They have an average of six tutors affili-ated to the PCT (5 are active), and Leads would dedicatean average of ... an average of four courses have beenplanned with a mean budget of £8,000. They have anaverage of two active tutors, and Leads dedicate an average of 15% of their working week to EPP. Finally, ... the key areas identified for action relates to the promotion of self-care. Self-care skills training has increasingly beenseen as an effective strategy for improving the quality of life and health...

Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf

... begins with the reduc-tive half-reaction involving the conversion of FAD(ﬂavin cofactor) to FADH2and concomitant oxidation of the amino acid to an imino acid. The imino acidintermediate of ... substrate analogsSomnath Mandal and Debasish BhattacharyyaDivision of Structural Biology and Bioinformatics, Indian Institute of Chemical Biology, Kolkata, India The ﬂavoenzyme l-amino acid oxidase ... allosteric ligand-bindingsite of predominant hydrophobicity.Binding of OAB in the substrate-binding site wasproposed to be similar to binding of the natural sub-strate in Ca. rhodostoma LAAO [12]....

Báo cáo khoa học: Kinetic and binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacteriumsp. strain HE5 ppt

... econazole andmiconazole to NHis-P450morwas too tight to analyseaccurately. In case of these three azoles, the opticalchange observed upon azole addition occurred linearlywith increasing azole ... CYP15 1A2 was obviously just derivedfrom the extrapolation of kinetic data and is thereforehard to compare with the turnover measured here. Onealso has to keep in mind that, in both cases, the assayconditions ... was used as a reference in HPLC analysis. The remaining reaction mixture was incubated for 30 min at30 °C and then terminated in the same way. Precipitatedproteins were removed by centrifugation.The...

Báo cáo khoa học: Endogenous mono-ADP-ribosylation mediates smooth muscle cell proliferation and migration via protein kinase N-dependent induction of c-fos expression potx

... (1.35-fold),however, the increase in cytosolic activity was only marginal(Fig. 3B).Activation of arginine-dependent mART would beexpected to lead to an increase in the post-translationalmodiﬁcation of select ... N., Nishina, H., Takahashi, K. &Katada, T. (1994) Characterization of botulinum C3-catalyzedADP-ribosylation of rho proteins and identiﬁcation of mammalian C3-like ADP-ribosyltransferase. ... examined. Their selection wasbased on information that each pathway is known to activate one of the key transcription factors that regulatesc-fos promoter activity. MAP kinase and PI3-kinase wereinvestigated...

Báo cáo khoa học: Lipid droplet and milk lipid globule membrane associated placental protein 17b (PP17b) is involved in apoptotic and differentiation processes of human epithelial cervical carcinoma cells pptx

... differentiation.Our previous data on the oncodevelopmental overex-pression of PP17b in prematurely aging epithelial-characterplacentas and squamous epithelial cervical dysplasias andcarcinomas indicated ... carcinomas. HeLacells and human milk are stained with anti-PP17 antibody. (A) In invasive squamous cervical carcinoma, tumour cells have punctuated,ring-like cytoplasmic PP17 staining (immunohistochemistry, ... PP17synthesis was notably elevated, which could be signiﬁcantly(*P <0.05)reducedbyPKAandPKCinhibitorsonlyincaseofPMA. Values indicated above the bars are the averages of threeseparate ﬂow cytometric...

Báo cáo khoa học: Redox reaction between amino-(3,4-dihydroxyphenyl)methyl phosphonic acid and dopaquinone is responsible for the apparent inhibitory effect on tyrosinase doc

... dopachrome.Changes in the UV/Vis spectra of a mixture of compound 1and Dopa oxidized by tyrosinase indicated that this wasindeed the case (Fig. 3C). In the initial phase of the reactionFig. 3. Spectral changes ... slow-binding inhibitor of catecholase activity of tyrosinase. Phytochemistry 26, 917–919.6. Hashiguchi, H. & Takahashi, H. (1977) Inhibition of two copper-containing enzymes, tyrosinase and dopamine ... We have not observed a decrease in the enzymatic activity during the course of the reaction (90 min) of tyrosinase with compound 1, whichindicated that covalent inactivation of the enzyme was...

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