báo cáo khoa học: "The creation of the health consumer: challenges on health sector regulation after managed care era" pdf

... F: On the Line New York: SEMIOTEXT[E]; 1983.doi:10.1186/1744-8603-7-2Cite this article as: Iriart et al.: The creation of the health consumer: challenges on health sector regulation after managed ... of 12RESEARC H Open Access The creation of the health consumer: challenges on health sector regulation after managed care eraCelia Iriart1*, Tulio Franco2, Emerson E Merhy3AbstractBackground: ... models, more centered on the needs of the patients than on the consumption of technical pro-cedures, are also operating and disputing the conceptua-lization of the health- ill -care model. It is...

báo cáo khoa học: "The creation of the health consumer: challenges on health sector regulation after managed care era" ppsx

... specification of minimum training,and other condition s for the medical profession. Suchregulations are likely to influence the ability of profes-sionals and establishments to supply health services ... this region and also the extent towhich the European Commissi on is in a position tonegotiate on behalf of the entire Union. The non-applic-ability of the services dir ective to health care reflects ... Recognition of ProfessionalQualifications. Official Journal of the European Union, L 255/22 Brussels;2005.9. Eur-Lex: Medicine: Mutual Recognition of Qualifications. Official Journal of the European...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... in the metal-bound ApeSOD. This implies that the conformationaround the active site of ApeSOD is independent of the presence of a metal cofactor.Figure 3D shows the superimposition of the ... °C(triangles) in a solution containing various concentrations of ethanol. The residual activities after the incubation are plotted against the ethanol concentration.Crystal structure of SOD from A. pernix ... apical positions. The manga-nese was only 0.06 A˚out of the equatorial plane(Table 3). The angles around the metal cofactor sug-gested that the ligation form of Mn in ApeSOD is tri-gonal bipyramidal.In...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... 6B)disappeared after the addition of 10 mm HU (ﬁnalconcentration) to R2F. Only the Mn(II) artefact wasobserved after the addition of HU (Fig. 6C). This sig-nal is similar to that of a control solution of ... is a strong promotorin C. ammoniagenes as well. Because of high expressionfrom the tac promotor, the proposed function of man-ganese in the transcriptional regulation of the nrdoperon [35] ... recipient.In the present study, we report data on homologousexpression of the nrdF gene of C. ammoniagenes strainATCC 6872. This is the ﬁrst report of the successfulpuriﬁcation of high amounts of the...

Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

... tocatalytic regulation, but also the thermostability of TtAK. Comparison of the crystal structures of TtAKband CgAKb was expected to elucidate the mechanism of the elevated thermostability of TtAKb.In ... AKs, only one of the eight-strandedb-sheets is involved in effector binding. Determination of the crystal structure of the regulatory subunit of TtAK (TtAKb) would provide information not only on ... effector-binding unit of the front of the dimer are indicated. Site 1and site 2 of the effector-binding unit on the front are indicated by solid and dotted cir-cles. (B) Superposition of two monomers ina...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... and E97 of the secondsubunit. The backbone NH group of R98 forms ahydrogen bond with the backbone CO of F102. Fur-thermore, the orientation of the side chain of the tworesidues brings the guanidinium ... interaction (Fig. 9B) [49]. The above discussion rationalizes the observed effects of the Y74W mutation in PfTIM on the stability of the dimeric structure and catalytic activity. Examination of the ... at13.9 mL, the position corresponding to the dimer, evenat the lowest concentration studied. Inspection of the gel ﬁltration proﬁle in Fig. 3 shows that the peak corre-sponding to the monomeric...

Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

... PH5CH8 cells. First, weconﬁrmed the effect of NS3-4A on the activation of the IFN-b gene promoter by the Cardif exogenouslyexpressed in PH5CH8 cells. The results of the lucif-erase reporter ... USA) were used fordetection of the phosphorylated IRF-3. The dimerization of exogenous IRF-3 was detected by anti-EGFP monoclonalserum (JL-8; Clontech).Preparation of PH5CH8 cells stably expressingHCV ... that the activation of IRF-3 by M-pIC treatment is mediatedby the TLR3 ⁄ TRIF signaling pathway. We obtainedfurther evidence by examining the status of the phos-phorylation and dimerization of...

Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... because they correspond to the regions inself-splicing introns that bind the exon substrates. The sites of catalysis for the ﬁrst step (substrate cleavage) and the second step(exon ligation) are ... isolate the ﬁrst reaction step(substrate cleavage) by preventing the second reactionstep (exon ligation). In addition, the product of the ﬁrst reaction step is actually the intermediate in the ... boundto the ribozyme, and [ribozyme]uis the concentration of unbound ribozyme in the reaction.Measurement of rate constant of substrate-cleavage product dissociation (k)3) The dissociation rate...

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... display nicely the interaction of the S1 site, butdo not interact with the S2 site of these proteinases.By contrast, the aspartates in positions P2 and P3 of the chloromethylketone occupy the S2 and ... for the function of mem-bers of this intriguing and emerging subfamily of serine proteases.Experimental proceduresCloning, expression and puriﬁcationCloning, expression and puriﬁcation of the ... bridgeCys191–Cys220 (the front of the pocket) (Fig. 4A). The backbones of these segments form a deep hydrophobicpocket with the negatively charged Asp189 at its bot-tom. Asp189 at the bottom of the pocket...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... b8-strand of one monomer into a b-sheet of the other monomer. The position of the zinc ion is indicated by acircle. (B) Schematic diagram of the dimer. Each monomer isshown with a different shade of ... On the other hand, the low degree of conservation in the loopregions near the active site could be associated withdifferences in the substrates used by the enzymes. The C-terminal regions of ... Thus, the conformational switch of the a2-helix could be of functional relevance and beassociated with either an ‘open’ nonfunctional confor-mation or a ‘closed’ conformation adopted by the acti-vated...

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