báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx

... 2011. doi:10.1186/gm284 Cite this article as: Yeo J-C, Ng H-H: Transcriptomic analysis of pluripotent stem cells: insights into health and disease. Genome Medicine 2011, 3:68. Yeo and Ng Genome Medicine 2011, 3:68 http://genomemedicine.com/content/3/10/68 Page ... pluripotent stem cells, pluripotency, regenerative medicine, therapy, transcriptional regulation, tra...

Ngày tải lên: 11/08/2014, 12:21

... a carpel into a fruit, and how these processes can take place in absence of pollination. Transcriptomic analysis of tomato carpel development and fruit set To identify the key steps and processes ... relevant and asses the impor- tance of GAs for parthenocarpic development and a new role for ethylene in this process. Results Transcriptomic analysis of tomato carpel...

Ngày tải lên: 12/08/2014, 03:20

... contributions TO conceived of the study and drafted the manuscript. HU carried out PCR and AFM. HK carried out AFM. AI par- ticipated in the design of the study and coordination. All authors read and approved ... 1: The detection of β-actin mRNA from single VNOf90 cells. The number of assays against single cells The number of β-actin positive cases The number of cells Th...

Ngày tải lên: 11/08/2014, 00:21

... acquired in H 2 O and D 2 O. Processing of the spectra was performed using the pro- gram TOPSPIN (Bruker). Analysis of the spectra, manual assignment of backbone and side-chain protons, and cross- peak ... pair of protons were converted into upper limits by cyana. Additionally, standard upper and lower limits for each of the two disulfide bonds (6–129, 57– 108) were intro...

Ngày tải lên: 18/02/2014, 08:20

... leaf, stem and flower at R1 and R4 developmental stage. A statistical analysis based on mean comparison by ANOVA and ordination by Principal Component Analysis allowed the detection of 80 candidate ... salinity Profile of gene expression by putative functional categoryFigure 5 Profile of gene expression by putative functional category. The number and direction of transcr...

Ngày tải lên: 12/08/2014, 05:20

... Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy 2 Department of Biotechnology, University of Verona, Italy 3 Department of Clinical and Biological Sciences, University of Insubria, ... Japan) and analyzed with imagemaster 2d platinum software, version 5.0 (GE Healthcare). Spots were detected automatically by the soft- ware and manually refined; gels were...

Ngày tải lên: 15/02/2014, 01:20

... site concentration of wild-type BuChE was 0.16 ± 0.01 lm, and that of the D70G mutant was 0.196 ± 0.02 lm. Effects of ligands on steady-state kinetics The effects of the ligands (L: tyramine, serotonin and ... ester). Results and Discussion Action of reversible effectors on AAA activity of BuChE The investigation of the effects of the ligands (tyramine, serotonin and b...

Ngày tải lên: 18/02/2014, 17:20

... plastoquinone; PS, photosystem; Q A and Q B , primary and secondary quinone acceptors of photosystem II; t 1 ⁄ 2 O–J , t 1 ⁄ 2 J–I and t 1 ⁄ 2 I–P , half-times of O–J, J–I and I–P phases, respectively. 4770 ... thylakoid membranes. Such analysis is needed for a a more thor- ough use of FI in the study of PSII electron transport and to obtain a more complete analysis of...

Ngày tải lên: 19/02/2014, 05:20

... structure of MCL, and effect of porphyrin binding CD spectra of MCL recorded in the far-UV region and near-UV region are given in Fig. 7A and 7B, respectively. Spectra obtained in the presence of a ... absorption bands. Fig. 2. (A) Absorption s pectra of CuTCPP in the absence and presence of different concentrations of MCL and (B) difference absorption spectra obtained b...

Ngày tải lên: 19/02/2014, 16:20

... ÔStains- AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4. As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional 2D gels, the expression level of ... viscosity of t he extract as a result of D NA, 2 lLof DNase I (200 units) was added per 100 lL of buffer [30]. Following filtration through two layers of miracloth a n...

Ngày tải lên: 19/02/2014, 16:20