báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

... J: Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease. Genome Medicine 2011, 3:36. Page 13 of 13 One genome, ... understanding of complex human diseases and their underlying genetic variants. © 2010 BioMed Central Ltd Epigenomics of human embryonic stem cells...

Ngày tải lên: 11/08/2014, 12:21

... (2 lg) of QGP-1 cells using random hexamers and Superscript III (Invitro- gen Corp.). One and two of 20 lL of the resulting single strand cDNA reaction were used as templates for RT-PCR and quantitative ... 40 cycles of 95 °C for 5 s, 60 °C for 20 s. Quantitative PCR was performed in a 20-lL reaction mixture containing 4 pmol of each primer, 10 lLof2· SYBR Premix Ex Ta...

Ngày tải lên: 19/02/2014, 06:20

... step- by-step formation of the disulfide bonds in PIP (Fig. 1B), disulfide bond formation in HPI occurs by random formataion of intramolecular disulfide bonds at the begin- ning of oxidized refolding, and ... concentration of denaturant. Fig. 3. Unfolding of HPI in the presence of denaturant and thiol cata- lyst. Controls for the disulfide scrambling method include the lack of...

Ngày tải lên: 19/02/2014, 12:20

... ± 44 nm for the wild type); EF-hand mutations 3 and 4 resulted in a complete loss of bind- ing (K D >5lm for hCaM-EF34 and hCaM-EF1234). EF-hand mutants of hCaM were also employed for functional ... in both cases, i.e. for BD-N as well as for BD-C2, lack of EF-hand motifs 1 and 2 only resulted in a mild reduction of binding (factor 7 and 9, respectively), while EF...

Ngày tải lên: 07/03/2014, 12:20

... Pain for editing the English and for continuous encouragement for our G. Anderluh et al. Stefin B and cellular toxicity FEBS Journal 272 (2005) 3042–3051 ª 2005 FEBS 3049 Interaction of human ... oligomers of vesicles made of acidic phospholipids resembles pore formation by A-b [27] and liposome permeabilization of a-synuclein [43]. The toxic activity exerted by prefi- bri...

Ngày tải lên: 07/03/2014, 17:20

... efficiencies intherangeof10 7 M )1 Æs )1 for UMP and CMP, and of 10 5 M )1 Æs )1 for AraCMP, L -3TCMP and dCMP (Table 1). Careful characterization of the reaction catalysed by human UMP-CMP kinase ... conditions. The amount of [ 3 H]CMP and [ 3 H]CTP formed in the presence of 1 m M [5- 3 H]CDP (0.2 CiÆmmol )1 ) and 0.25– 2m M of dCDP or L -3TCDP was monitored for 2,...

Ngày tải lên: 08/03/2014, 02:20

... syto 9 (for live bacteria) and propidium iodide (for dead bacteria). An aliquot of 1 mL of exponential phase cells was incubated with 5 l M of each protein. Duplicates were performed for each ... pres- ence of a hydrophobic patch and the capacity of the protein to bind LPS. An understanding of the molecular mechanism that is responsible for the high binding affinity o...

Ngày tải lên: 22/03/2014, 21:20

... Effect of DON on expression of MLL1 and HoxA7 genes in presence of MAP ⁄ Src kinase inhibitor PP2 and PD98059. H358 cells were treated 0.1, 1, 10 and 25 l M PP2 and 1, 5, 25 and 50 l M PD98059 for ... significantly cytotoxic towards human cells. Src kinase inhibitor suppressed the DON -induced upregulation of MLL1 To understand potential mechanism of DON-mediated u...

Ngày tải lên: 23/03/2014, 04:21

... RNAi-mediated depletion of both U2AF 35 a and U2AF 35 b isoforms in HeLa cells altered alternative splicing of Cdc25 transcripts [55]. Sequence comparisons of U2AF 35 splicing isoforms and U2AF 35 -related ... after mice and humans diverged, because the human ortho- log of Murr1 is located on chromosome 2 and there are no U2AF1-related genes on human chromosome 2. Indeed...

Ngày tải lên: 23/03/2014, 10:20

... Gulino 2 and Andrew R. Mackay 1 1 Section of Molecular Pathology, Department of Experimental Medicine, University of L’Aquila, Italy; 2 Department of Experimental Medicine and Pathology, University of ... vitro and we discuss the potential mechanism involved. MATERIALS AND METHODS Cells and reagents Human MDA-MB-231 breast carcinoma cells were obtained from the Ameri...

Ngày tải lên: 31/03/2014, 09:20