báo cáo khoa học: " Functional genomics and rheumatoid arthritis: where have we been and where should we go?" pdf

... rheumatoid arthritis. © 2010 BioMed Central Ltd Functional genomics and rheumatoid arthritis: where have we been and where should we go? James N Jarvis 1 * and Mark Barton Frank 2 R E VIE W *Correspondence: ... us, any survey of where we have gone and where we might go in the use of systems biology and functional genomics to understand RA must...

Ngày tải lên: 11/08/2014, 12:20

... mutants Genes paaF, paaH, paaI, paaJ and paaZ were targeted in E. coli K12 as previously described [11]. All gene mutations were verified by PCR and sequencing (Microbiology and Molecular Genetics Core ... 0.3 m M ) were added to 15 mL of cell suspension. The mixtures were incubated at 30 °C under shaking (180 r.p.m.). Samples were retrieved at intervals and centrifuged. The supernatan...

Ngày tải lên: 23/03/2014, 18:20

... reconstituting recombinant human CLIC1, CLIC4 and CLIC5 in pla- nar lipid bilayers. CLIC1 and CLIC4 have previously been characterized at the single-channel level, and, in this study, we confirmed for the first time ... channels that were almost equally permeable to Na + ,K + and Cl – , suggesting that the ‘CLIC’ nomenclature may need to be revised. CLIC1 and CLIC5, but not CLIC4...

Ngày tải lên: 30/03/2014, 04:20

... SDF-1 ⁄ CXCL12 and fractalkine ⁄ CX3CL1, are also known to have angiogenic proper- ties [44,58,59]. Recently, associations between the chemokine gene polymorphisms and RA have been investigated. ... isoform of CCL3 encoded by different genes and a potent ligand for CCR1 and CCR5, influenced susceptibility to RA, and genetic interaction between CCL3L1 dose and CCR5D32 was also...

Ngày tải lên: 18/02/2014, 18:20

... synoviocytes and the development of collagen-induced arthritis [53]. Weather conditions Although no good biologic hypotheses have been proposed that might explain the association between RA and weather ... genetic and environmental factors. Genetic influences that contribute to RA susceptibility have been demonstrated both in studies of twins and families, as well as in genome-...

Ngày tải lên: 18/02/2014, 18:20

... were stimulated with 10 ng ÆmL )1 of IL-1b. Interleukin-6, IL-8 and GM-CSF production from FLS were suppressed in a dose-dependent man- ner in the presence of PPAR-c ligands and a PPAR-a ligand, ... hyperlipidemia and hypertension, there are some drugs that have the potential to inhibit NF-jB activation. To seek other candidate compounds for use in an anti-RA strategy, we studied se...

Ngày tải lên: 18/02/2014, 18:20

... (AP1, Elk1, Stat1, SRF and NFκB) and expression of apoptotic genes (Fas receptor, Fas ligand, FADD and caspase 8) [19]. In addition, as confirmed by the functional genomics analysis presented ... STAU2, and HMGN3) genes and one down-regulated (ANK3) gene. All these genes exert their function on transcriptional and translational processes and until now none of these genes...

Ngày tải lên: 31/10/2012, 15:28

... and biochemicals were of reagent grade or better, and were used without further purification. Compounds 1–3 [49,50] and 4–7 [51] were synthesized as described previously, and were generous gifts ... they were found to be highly soluble, and resistant to both enzyme-catalyzed and nonenzymatic hydrolysis [28,29]. Unlike this previ- ous study, however, we chose to examine sulfonamid...

Ngày tải lên: 14/02/2014, 22:20

... purines and negative cooperativity towards dThd and dCyd was observed. The Q81N mutation showed a 200- and 100-fold increase in K m , whereas k cat was decreased five- and twofold for dThd and dCyd, ... 210–230. The loop connecting a9 and b5 (residues 195–200) was flexible and poor density was observed. The electron density for the mutant resi- due and the ligand was well defined...

Ngày tải lên: 19/02/2014, 02:20

... 6, Glu-Pg- and mAb 34D3-trea- ted cells. Bands in lanes 1 and 2 correspond to forms I and II of plasmin(ogen). High molecular weight bands in lanes 5 and 6 correspond to mAb A10.2- and mAb 34D3-plasminogen ... conformations and LBS accessibility to its functional properties such as binding to fibrin and cells, activation on these surfaces and the effect of plasmin proteolytic...

Ngày tải lên: 20/02/2014, 01:20