báo cáo khoa học: " Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase" ppsx

... ied a Haspin kinase, designated AtHaspin, in Arabidopsis thaliana. The purified AtHaspin phosphorylated histone H3 at both Thr3 and Thr11 in vitro. Live imaging of AtHaspin-tdTomato and GFP -a- tubulin in ... aurora-like kinases in Arabidopsis and other plants. Plant Cell 2005, 17(3):836-848. 11. Kawabe A, Matsunaga S, Nakagawa K, Kurihara D, Yoneda A, Hasezawa S, Uchiyam...

Ngày tải lên: 11/08/2014, 11:22

... pGEM-T clone (GenBank:DQ335596) as a template and the following pri- mers: CaNDR1-BglII 5’-TCAGATCTTATGGACA AAG- GATGGGGC-3’,andCaNDR1-BstEII 5’-T AGGTCAC CAAATTAATTCCCAGGAAA-3’.DigestedPCRpro- ducts ... GAC GTA CCA GAT TAT ATG TC AGA CCC CAG CAG CAG TGC-3’ and CaNDR1-Reverse 3, 5’-CTA ATG GTG ATG GTG ATG GTG CAA CAG CAG AAC CAA GAA A- 3’. The primers used for obtaining the single HA...

Ngày tải lên: 11/08/2014, 11:21

... Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono ... Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura...

Ngày tải lên: 11/08/2014, 11:21

... lemma and palea and is an early-acting regulator of inner floral organs. Plant J 2005, 43(6):915-928. 74. Prasad K, Sriram P, Kumar CS, Kushalappa K, Vijayraghavan U: Ectopic expression of rice ... related to APETALA1 and LEAFY of Arabidopsis. Plant Physiol 2001, 125(4):1788-1801. 73. Prasad K, Parameswaran S, Vijayraghavan U: OsMADS1, a rice MADS-box factor, controls differ...

Ngày tải lên: 11/08/2014, 11:22

... role as a latency-associated gene. Identified in yeast two-hybrid screening and verified by pull-down assays, WSSV403 is able to bind to a shrimp protein phosphatase (PPs), which was characterized ... used, 403RING can be strongly activated as an E3 ligase by Pvubc, ubcH3, ubcH 5a, ubcH5c and ubcH6 (Fig. 2B), indicating that 403RING can support E3 ligase activity and display a...

Ngày tải lên: 12/08/2014, 04:21

... sugars of three classes. Whereas agglutination was inhibited by galactose and its deriva- tives [such as N-acetylgalactosamine (GalNAc), methyl -a- d-galactopyranoside], it was evident that, at doses ... within the last 30 years. The greatest number of RIPs have been found in the Caryophyllaceae, Sambucaceae, Cucurbitaceae, Euphorbiaceae, Phytolaccaceae and Poaceae [1]. Although man...

Ngày tải lên: 18/02/2014, 16:20

... GTT GGA ATT CCA TCA TCA TCA TCA TCA TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAG GAG GGA TAT GGG GAA C. The PCR reaction was done as described above. The amplified ... nature. Tyrosinases are found in prokaryotic as well as in eukaryotic microorganisms, and in mammals, inverte- brates and plants. Tyrosinase is a mono-oxygenase and a bifunctio...

Ngày tải lên: 19/02/2014, 06:20

... C)AATTTC-3¢ PsCBL (degenerate forward) 45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ PsCBL (degenerate reverse) 55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward) 65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR ... T)GC(C ⁄ G ⁄ T)AAGGT-3¢ PsCIPK (degenerate forward) 25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate reverse) 35¢-CTTAT(C ⁄ G)AACAAGGAA (A...

Ngày tải lên: 19/02/2014, 07:20

... PCR was performed with the M13 forward primer (5¢-CCCAGTCACGACGTTGTAAAA CG-3¢)asasenseprimerandHSF1-specific primers (for HSF 1a, 5¢-GAAGCAGCTTGTCCAGTACACTAA-3¢;for HSF1b,5¢-GAAGCAGCTGGTCCAGTACACCTC-3¢ )as antisense ... Japan) and reared on a commercial diet at 15 °C. Cloning of HSF cDNA A random primed kZAPII cDNA library was constructed by using a kZapII predigested EcoRI/calf int...

Ngày tải lên: 19/02/2014, 12:20

... was inhibited not only by unlabeled Bip-Pro itself, but also by well known sub- strates of H + ⁄ peptide cotransporters, such as Gly-Sar, Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala, d-aminolevulinic ... (Fig. 1A) and the synthe- sis strategy (Fig. 1B) of Bip-Pro. The purity of the compound was assessed by TLC, analytical RP- HPLC, MS and NMR, and was found to exceed 98%....

Ngày tải lên: 07/03/2014, 05:20