báo cáo khoa học: " Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine" pptx

... -GCAGAAGTTGCCCTCATCGT-3’ and 5’ -AAGC- CAAGGAATCACCCATT-3’; for the internal reference gene EF1 -a (GSVIVT00024496001-8.4x ) the oligos are 5’ -AGGATGGACAAACCCGTGAG-3’ and 5’-AAGC- CAGAGATGGGGACAAA-3’, ... design; performed the genotyping, phenotyping, sequence and database analyses; performed marker design and statistical, linkage and QTL analyses; and designed and drafted t...

Ngày tải lên: 11/08/2014, 11:21

... efficient and safe therapies. Abbreviations ad: autosomal dominant; adCORD: autosomal-dominant cone and rod dystrophy; adLCA: autosomal dominant LCA; adMD: autosomal-dominant macular dystrophy; adRP: ... congenital amaurosis Mainly ar, rarely ad Stargardt disease Mainly ar, rarely ad Fundus avimaculatus ar Congenital stationary night blindness ad, ar, xl North Carolina macular dystr...

Ngày tải lên: 11/08/2014, 12:20

... The formation of clathrin-coated vesicles are gen erated by the joint action of machinery that includes clathrin heavy and light chains, adaptor complexes (AP1 and AP2), membrane-bending proteins ... loss -of- functional gene analysis in a rapid and cost-effective manner. For this purpose, we have established and validated a RNA interference screening platform assay that a...

Ngày tải lên: 12/08/2014, 04:21

... primers oligonucleotide Perennial ryegrass ESTs and GenBank-derived clones Bioinformatic annotation for functional role Amino acid alignment R-gene characterisation and classification into distinct classes and families Candidate ... of an expressed sequence tag (EST) resource and a functionally-integrated bioinformatics database. Results: Amplification of R gene sequences...

Ngày tải lên: 12/08/2014, 03:20

... cDNAs by PCR using the primers 5¢-GACGACGACA AGATGGAGGTTAAGGATGAGTTG-3¢ and 5¢-GAG GAGAAGCCCGGTCTATAACTCATCTTTGAGTAC-3¢ for GmPDIL- 3a, and 5¢ -GACGACGACAAGATGGAGG TTGAGGATGAGTTGG-3¢ and 5¢-GAGGAGAAGCCCG GTTCATAACTCATCTTTGACGAC-3¢ ... 5¢-CG TTTGAAGGGTGAGGAGGAAAA[FAM]G-3¢ and 5¢-CA CAAGAGAGTTCTGCGATAACCTTG[FAM]G-3¢ (Invi- trogen Corporation) and reverse primers 5¢-AAGTAGGCA ACAAAAC...

Ngày tải lên: 18/02/2014, 11:20

... 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu- bating the protein at 60, 70 and 80 °C for 30 min and then recording ... replicative primases (AEPs) [11]. Primase–polymerases (prim–pols) are a novel family of AEPs which are sporadically found in both bacterio- phages and crenarchaeal and Gr...

Ngày tải lên: 18/02/2014, 18:20

... 5¢ATGGCCACTCAGTATGACC, FF5, matching with the N-terminal sequence of the mature protein: 5¢TAT GCGTGGTCCAATGCTATGC, and FF 3A, matching with the C-terminal sequence of the coding region: 5¢TTAC AAGGACAAATTAATTGTGCCAG. ... streptavidin conjugated with horseradish peroxidase instead of alkaline phosphatase was used. After visualization of the enzymatic activity with tetramethylbenzidi...

Ngày tải lên: 21/02/2014, 00:20

... blot using the rabbit a- Scpep1 antibody. A mixture of molecular mass standard proteins, including IgG (160 kDa), albumin (67 kDa), ovalbumin (43 kDa) and ribonuclease A (13.7 kDa), was applied ... 54, 755–764. 25 D’Azzo A, Hoogeveen A, Reuser AJ, Robinson D & Galjaard H (1982) Molecular defect in combined beta- galactosidase and neuraminidase deficiency in man. Proc Natl...

Ngày tải lên: 07/03/2014, 03:20

... 5¢-GAGGAGA AGCCCGGTTCAAAGCTCATCTTTTCCTTTTTC-3¢ for GmPDIL-1, and 5¢-GACGACGACAAGATGCTCACCGA CGACGAGGACC-3¢ and 5¢-GAGGAGAAGCCCGGTTC ATAATTCATCCTTCACATC-3¢ for GmPDIL-2. Ampli- fied DNA fragments were subcloned into ... as follows. DNA fragments were amplified from cDNAs of GmPDIL-1 and GmPDIL-2 by PCR using the oligonucleotide primers 5¢-GACGACGACAAGATGGAG GAATCATCGGAGAAAGAGTTC-3¢...

Ngày tải lên: 07/03/2014, 05:20

... GTCGTAATTAATCACTTAACCGTGCTCG CYP71 9A2 reverse GAAAGAAACAGAGCAAATCTTATCCTTTTACC CYP71 9A3 forward CCTCGTAACTAATATACCAGTGTGGTG CYP71 9A3 reverse GACAACCAAGCAAACTCTTATTCTTGTAC Internal control gene b-actin ... (5¢-GTTATGGTGTAAGGCATAAAGATTA ACCATAACC-3¢) and 5¢-GSP1 nested (5¢-TGTAAGGCA TAAAGATTAACCATAACCCTAGTACC-3¢) and 3¢- RACE, 3¢-GSP1 (5¢-AATAAGGGTACTAGGGTTATGG TTAATCTTTATGC-3¢) and 3¢...

Ngày tải lên: 07/03/2014, 10:20