báo cáo khoa học: " Two novel types of hexokinases in the moss Physcomitrella patens" ppsx

... hexokinases have lost intron 2,which is present in the other moss hexokinases, includ-ing the type D hexokinases (Figure 1). Finally, we notethat the sequence of the type C hexokinase, PpHxk4, ismore ... of the Physcomitrella hexokinases We proceeded to study the intracel lular locations of the moss hexokinases. Sequences from the new hexokinases, expressed from the 35S promoter, were fused in ... also involved in sugarsensing in both fungi and plants. We have previously described two types of hexokinases in the moss Physcomitrella. Type A, exemplified by PpHxk1, the major hexokinase in Physcomitrella, ...

Báo cáo khoa học: Two different types of hepcidins from the Japanese ﬂounder Paralichthys olivaceus ppt

... that of human,mouse and other reported ﬁsh hepcidins. In liver, somecells expressed both types of hepcidins, while othercells expressed only one of them or neither of them(Fig. 6). These ... type II hepcidin of winter ﬂounder. Both genesexist as single copies in the Japanese ﬂounder genome. The structures, and therefore the functions, of Hep-JF1and type II hepcidin of winter ﬂounder ... Alignment of amino acid sequences of Japanese ﬂounder and other reported hepcidins. The box indicates the mature peptide region.Hashes below the alignment indicate the positions of cysteine residues....

Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

... to the puri-ﬁed protein (assuming no major change in the extinc-tion coefﬁcient of the bound cofactor in the case of the mutants) [15]. The peak absorbance ratios showedﬁrst of all that the ... bio-chemical outcome of these point mutations.Results The effect of chaperonin on the ability of the mutant proteins to foldFor a number of other MCAD point mutations,co-overexpression of the GroELS ... for the inactivity of the R256Tmutant protein.Thermostability of the puriﬁed mutant proteins The effect of temperature on MCAD stability wasdirectly investigated by incubating aliquots of eachpuriﬁed...

Báo cáo khoa học: "Two different types of malignant fibrous histiocytomas from pet dogs" docx

... MFH rarely occurs in young animals. In addition, the second case showed typical findings of the inflammatory cell type of MFH with an ossifying matrix resulting from continuous fibroblast ... surrounding neoplastic fibroblasts in the central region of the lesion. The current report may be important in identifying MFHs based on histologic and immunohistologic findings and classifying the ... calcifications in a young healthy dog and compared of the findings to another case of inflammatory MFH. A two- month old female Shih-tzu was brought to a local veterinary clinic with a raised...

Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterﬂy, Papilio xuthus docx

... staining of the gel indicates that the ﬂuorescing protein is one of the major components of soluble proteins in the crude extract. The surface of the ﬂuorescing protein carries negative charge in ... cellular retinal-binding protein; CRBP,cellular retinol-binding protein; IRBP, interphotoreceptorretinoid-binding protein; RBP, retinol-binding protein.Note: The nucleotide sequence reported in this ... both in the distaland proximal portions of the retina. In contrast, the increase of 11-cis ligand was observed in the distal but not in the proximal portion of the retina. Together with the resultsfrom...

Tài liệu Báo cáo khoa học: A possible role of mitochondria in the apoptotic-like programmed nuclear death of Tetrahymena thermophila Takashi Kobayashi and Hiroshi Endoh docx

... which appear to be involved in the ﬁnalresorption of the parental macronucleus during PND in T. thermophila, and suggested the involvement of mitochondria in this process [19]. In mammalian apop-tosis, ... directlyshow the localization of mitochondria in the autophag-osome, as the red ﬂuorescence corresponding to intactmitochondria was not observed in the area. Therefore,to conﬁrm further the localization, ... stained the degenerating parental macronucleus, but not the othernuclei (Fig. 3C–E). Moreover, the density of stainingwas high around the degenerating macronucleus, pre-sumably corresponding to the...

Tài liệu Báo cáo khoa học: A novel type of highly negatively charged lipooligosaccharide from Pseudomonas stutzeri OX1 possessing two 4,6-O-(1-carboxy)-ethylidene residues in the outer core region ppt

... found either in wild-type strains and in mutant strains harboring mutations in the genes encoding enzymes of the biosynthesis and/or the transfer of the O-speciﬁc polysaccharide [12,13]. The core ... O-6 of the glucose residuesubstituting the pyruvate moiety. This ﬁnding wouldconﬁrm that pyruvate residues are used to block chainelongationintheLOSofP. stutzeri OX1.Thus, the structure of the ... Y.A.,Za¨hringer, U. & Pier, G. (2003) Elucidation of the structure of the lipopolysaccharide core and the linkage between the core and the O-antigen in Pseudomonas aeruginosa immunotype 5 using...

Tài liệu Báo cáo khoa học: "Some Novel Applications of Explanation-Based Learning to Parsing Lexicalized Tree-Adjoining Grammars"" doc

... Table 2. The size of the FST obtained for each of the corpora, the coverage of the FST and the traversal time per input are shown in this table. The cover- age of the FST is the number of inputs ... level of generalization of the parses in the training set, thereby dealing with test sentences which are not necessarily of the same length as the training sentences and (c) represent the set of ... sentence. The application phase of EBL is shown in the flowchart in Figure 4. An index using the morphological features of the words in the input sentence is computed. Using this index, a set of...

Báo cáo khoa học: A novel mechanism of V-type zinc inhibition of glutamate dehydrogenase results from disruption of subunit interactions necessary for efﬁcient catalysis doc

... 2.Effects of zinc on cofactor binding The effects of zinc on the binding of the reduced co-factor NADPH to the enzyme, at pH 8.0, in the pres-ence or absence of glutamate were examined usingﬂuorescence ... part of the $ 18° movement of the NAD binding domainduring catalysis [30,31]. Therefore it is possible thatzinc, binding here, could mimic the effects of GTPbinding to this location. The other ... demon-strate that zinc binds to the enzyme in the absence of other ligands or in the presence of glutamate or norva-line – the lack of inhibition of the norvaline-dependentreduction of NAD(P)+is clearly...

Báo cáo khoa học: A novel metallocarboxypeptidase-like enzyme from the marine annelid Sabellastarte magniﬁca – a step into the invertebrate world of proteases pdf

... vorepre-sents the enzymatic activity in the absence of the inhibitor. The experimental points were adjusted to the equationdescribed for tight binding inhibition [48] by employingnonlinear ﬁtting using ... pmol (1 lL) of CyDye per 30 lg of protein, incu-bated on ice for 30 min in the dark and quenched with1 lLof10mm lysine and then incubated on ice for 10 min in the dark, according to the manufacturer’s ... (lowerpart). In Fig. 3, we show, in the uncombined(Fig. 3A,B) or in the combined way (Fig. 3C), the pro-tein components of both parts of the animal labeledwith ﬂuorescent dyes using the DIGE approach....

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