báo cáo khoa học: " Identification of potential target genes for the tomato fruit-ripening regulator RIN by chromatin immunoprecipitation" pdf

... cells by screening the promoters of the genes that are highlyupregulated during ripening and also within the DNAfragments prepared by the ChIP with the ant i -RIN anti-body. Results for the RIN target ... 11:26http://www.biomedcentral.com/1471-2229/11/26Page 3 of 14RESEARCH ARTIC LE Open Access Identification of potential target genes for the tomato fruit-ripening regulator RIN by chromatin immunoprecipitationMasaki ... to these CArG boxes. Genes in the flanking regions of the RIN- binding CArGboxes and their expressionTo detect potential target genes of RIN, we analyzed the 5-kb genomic regions flanking the...

báo cáo khoa học: " Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism" pps

... investigate the roles played by these genes during viralinfection, specifically addressing the means by which these genes influence the accumulation of BaMV protein.Nine of the 15 genes showed either ... influence on the accumulation of BaMV.Knocking down the expression levels of these host genes using the VIGS system enables the identification of the novel functions of genes in pathogen-host inter-actions. ... Tsai1,2*AbstractBackground: The genes of plants can be up- or down-regulated during viral infection to influence the replication of viruses. Identification of these differentially expressed genes could shed light on the...

báo cáo khoa học: " Identification of drought-response genes and a study of their expression during sucrose accumulation and water deficit in sugarcane culms" potx

... stress-related genes in diffe rentparts of the sugarcane culm raise s the question of the role of these genes in maturing sugarcane internodes.One hypothesis is that the expression of stress-related genes, ... in the culm. The expression of this sub-set of genes was subsequently examined inplants of one cultivar undergoing w ater deficit. The functional identity of these genes provides a basis for the ... regulation. Of the 51 genes tested, 17 genes showed higher expression in the mostmature internodes of the culm than in the leaf. Of these17 genes, nine were more highly expresse d in the olderinternodes,...

báo cáo khoa học: " Identification of rhizome-specific genes by genome-wide differential expression Analysis in Oryza longistaminata" doc

... internodesFigure 2 The plot of the first principal components of the genome-wide gene expressi on profile of five tissues in O. longistaminatarevealed by the microarray expression analysis. PC1 is principal ... prediction of cis-actingregulatory elements for the tissue-specific genes The putative function of each tissue-specific gene corre-sponding to the probe set on the chip was predicted by the Affymetrix ... Array.Word file for the list of genes enriched in the shoot internode of Oryzalongistaminata and their function annotation.Additional file 6: The list of 1974 genes specifically enriched in the young...

báo cáo khoa học: " Identification of differentially expressed genes associated with semigamy in Pima cotton (Gossypium barbadense L.) through comparative microarray analysis" docx

... of the biologically active(-)-enantiomer than the majority of other cotton species;these of the species produce more of the biolo gicallyinert (+)-enantiomer than G. barbadense [30,31]. The compound ... semigametic anthers (Table 1). The down-regulation of actin in semigametic ovules maycause the misalignment of the sperm cell and inhibition of sperm movement. Even though the function of micro-tubules ... perform six qRT-PCR reac-tions to study the expression of R ubisco i n semigameticversus non-semigametic anther and ovule tissues. The results of the reactions are presented in Figure 3. Of the six...

Báo cáo khoa học: "Application of pulsed Doppler ultrasound for the evaluation of small intestinal motility in dogs" pps

... information. In spite of this, thesetechniques are unsuitable for large-scale clinical studiesdue to their complexity [19, 24]. The method of monitoring contractions by placing sen-sors on the ... During the early scanningperiod, the B-mode was used for locating the optimalbowel loop, and then the mode was switched to the pulsedDoppler mode. The sample volume cursor was located in the ... through the acoustic window of the spleen. The number of peristaltic contractions wasrecorded three times over a one-minute time period and the average number of contraction was then calculated.The...

Báo cáo khoa học: " Development of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies" potx

... to monitor the development of the antibody level using the yolk diluted 1,000 times with PBS-T. The yolk antibody also showed no cross reaction to anyother bacteria (Table 1), and the antibody ... levels of all groupsincreased on 30 days after the first immunization. Group 2(200µg) showed the antibody titers comparatively higherthan those of the other two groups (data not shown). The ... wascultured at 22oC or 30oC for 24h and these cultures werediluted from 108 to 1 cells/ml in TSB. From these tubes 0.1-1ml were calculated the number of L. monocytogenes by plate count method...

Báo cáo khoa học: "Evaluation of a blocking ELISA for the detection of antibodies against Lawsonia intracellularis in pig sera" docx

... participated in the design of the study. MJ wereresponsible for the coordination of the work, for collecting the samples, for the PCR analyses and drafted the manuscript. PW were responsible for the serological ... Figure 1. The intersection of the lines corresponds to the cut-off value for the ELISA testrecommended by the manufacturer, i.e. PI 30. The med-ian of the posterior estimates of the sensitivity ... for the sensitivity of the IFATwas 88% and the specificity was 95%. The estimateswere very stable irrespective of the cut-off used for the ELISA test.Using PI 30 as cut-off, the IFAT and the...

Báo cáo y học: "Identification of signaling components required for the prediction of cytokine release in RAW 264.7 macrophages" pdf

... inpredicting the release of one cytokine (IFNβ for IL-6, IL-6 for IL-10 and M-CSF for TNFα). Figure 6 displays the fits of thesedifferent minimal models for training and test data. Most of the training ... the elucidation of the common and different signaling modules required for the release of different cytokines, and the quantitative prediction of amounts of cytokines released. The Alliance for ... Thus,understanding the regulation of the expression and release of these mediators is crucial for understanding the course of the inflammation process. Here we propose models that derive the responses of seven...

báo cáo khoa học: " Identification of miRNAs and their target genes in developing soybean seeds by deep sequencing" doc

... located in the CDS of target genes (Table 3). Because genes with full-lengthcDNA represent only 5% of all predicted genes in the soybean database [7], the genes slice d by miRNA in the UTR region ... was the key regulator of these genes. In most cases, targets of the same miRNA belong to the same gene family (Table 3); however, some miRNAs,such as gma-miR398, can target three types of genes, including ... moreclues to the different regulations of gene expressionsbetween species.We further studied the target genes of the miRNAs by degra dome library sequencing. Unlike other species stu-died by degradome...

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