báo cáo khoa học: " High levels of nucleotide diversity and fast decline of linkage disequilibrium in rye (Secale cereale L ) genes involved in frost response" doc

báo cáo khoa học: " High levels of nucleotide diversity and fast decline of linkage disequilibrium in rye (Secale cereale L.) genes involved in frost response" doc

... Access High levels of nucleotide diversity and fast decline of linkage disequilibrium in rye (Secale cereale L. ) genes involved in frost responseYongle Li1, Grit Haseneyer1, Chris-Carolin Schön1, ... well-suited for allele mining in genes involved in the frost responsive network. For investigating genetic diversity and the extent of linkage disequilibrium (LD) we analyzedeleven candidate genes ... (dN/dS ), number of Indels and haplotypes, haplotype (Hd) and nucleotide diversity ( ), Tajima’s D, and linkage disequilibrium (LD)CG Fragment length(bp)GenecoverageaNo. of linesbNo. of SNPsc(non-synonymous)dN/dSNo....

Báo cáo khoa học: High levels of structural disorder in scaffold proteins as exempliﬁed by a novel neuronal protein, CASK-interactive protein1 pot

... several hundred proteins, including recep-tors and channels, cell adhesion proteins, cytoskeletalproteins, G-proteins and their modulators, and signal-ling molecules including kinases and phosphatases ... directly related to the involvement of disor-der in scaffold proteins. In fact, there is an elevatedlevel of disorder in hub proteins, i.e. proteins involved in multiple interactions [13–16], and ... are oftendirectly involved in protein–protein interactions [42],these points help us to interpret the possible role of disorder in Caskin1 and in other scaffold proteins. Toobtain a balanced...

Báo cáo khóa học: High level cell-free expression and speciﬁc labeling of integral membrane proteins doc

... wereconsiderably higher. After puriﬁcation and solubilizationinto detergent micelles, we could calculate a yield of resolubilizable protein of 1 mgÆmL )1 RM for YﬁK,1.5 mgÆmL )1 RM for SugE and of 2.7 ... GFPexpression. L -a-phosphatidylcholine (LPC), 1,2-dimyris-toyl-sn-glycero-3-phosphocholine (DMPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POGP) and an E. colilipid mixture were added in increasing ... of the solubilization of SugE,YﬁK and TehA in micelles and of their reconstitution intomembranes. The dissolving of the proteins in suitabledetergents obviously resulted in the refolding of...

Báo cáo khoa học: " High-risk surgical stage 1 endometrial cancer: analysis of treatment outcome" pdf

... ( %) total ( %) Initial failure site 30 76Vaginal 3 (1 0) 0 3 (2. 8) Pelvic 5 (1 7) 0 5 (4. 7) Distant 22 (7 3) 0 22 (20. 7) Alive 10 (7. 9) 70 (92. 1) 80 (75. 8) Outcome Patients relapsesYes No total ... < 0.000 1). In multivariate analyses theinitial failure in distant sites (p < 0.000 1) was the only factor associated with poor survival. Acute and chronic gastrointestinal and genitourinary ... C Pellizzon - marcelmelo@gmail.co; Marcel D De Melo - marcelmelo@gmail.com; Paulo E Novaes - marcelmelo@gmail.com; Ricardo C Fogaroli - rcfogaroli@aol.com; Maria A Conte - contemaia@uol.com;...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... over-expressing subline(K562 ⁄ ADR), the GLC4 cell line and its MRP1 over-expressing subline (GLC4 ⁄ ADR), the 2008 cell line and its sublines over-expressingMRP1, MRP2 or MRP3, and the cell line HEK293 ... pumping of the agents out of the cells and theirintracellular binding to receptors and ⁄ or pumping intointracellular compartments.Because the rhodamines were excluded from all celllines ... (Sigma-Aldrich). GLC4 cells and MRP1 over-expressing GLC4 ⁄ ADR cells [47] werecultured in RPMI 1640. All media were supplemented with10% fetal bovine serum, 100 IUÆmL )1 penicillin and 100 lgÆmL )1 streptomycin...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... on LB agar plates containing ampicillin(50 mg L )1 ), single colonies were picked for 2 mL over-night cultures in LB medium containing ampicillin(50 mg L )1 ), and plasmids were prepared using ... cells (BL21DE3 PLysS Star) by heat shock and spread on LB agarplates containing ampicillin (50 mg L )1 ) and chlorampheni-col (30 mg L )1 ). Single colonies were used to inoculate50 mL overnight ... standard deviation. (A) Ab(1–4 0) (open triangle) and Ab(M1–4 0) (inverted open triangle); (B) Ab(1–4 2) (closed triangle) and Ab(M1–4 2) (inverted closed triangle). To assess the effect of prolonged...

Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx

... istightly linked to their thermodynamic stability.Methods for studying quadruplexfolding and unfoldingThe folding and unfolding of G-quadruplexes can beconveniently monitored using spectroscopic ... Methods Enzymol 180, 304–325.30 Mergny JL & Lacroix L (200 3) Analysis of thermalmelting curves. Oligonucleotides 13, 515–537.31 Mergny JL, Phan AT & Lacroix L (199 8) FollowingG-quartet ... of several kilobases of tandem repeats of the sequence 5¢-TTAGGG, includ-ing a terminal single-stranded overhang of $ 200nucleotides. This overhang can fold into a variety of quadruplex structures,...

Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-speciﬁc intronic inverted repeats pptx

... shockdomain protein LIN-28 controls developmental timing in C. elegans and is regulated by the lin-4 RNA. Cell88, 637–646.18 Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE,Bettinger JC, ... the high frequency of LIRs in long genes. A total of 956LIRs (in 702 genes) were located in genic regions, and 1595 in intergenic regions. In other words, 37% of the LIRs were found within genes. ... of TG ⁄ CA dinucleotides and interspersed TA dinucleotides. We deﬁned them asTG ⁄ CA-rich LIRs, accounting for 33% and 39% of all the LIRs in the intronic and intergenic regions,respectively...

Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx

... Z-Leu-Leu-Leucinal (Z-LLnL,MG13 2), N-benzyloxycarbonyl- L -leucinyl- L -leucinyl- L -leucinal;Z-Leu-Leu-Norvalinal (Z-LLnV, MG11 5), N-benzyloxycarbonyl- L -leucinyl- L -leucinyl- L -norvalinal.Enzyme: ... Biochem. 27 1) Ó FEBS 2004was inhibited 12 h postinduction by addition of thecommercially available inhibitors lactacystine, Z-Leu-Leu-Norvalinal or Z-Leu-Leu-Leucinal to the S2 cells stablytransfected ... equal volume of the denaturing SDS buﬀer and loadedonto a single lane. Activity levels are indicated as follows: ( +), measurable NAAG-hydrolyzing activity; (+/ ), extremely low activ-ity; ( ), ...

Báo cáo khoa học: Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identiﬁed by site-directed mutagenesis pot

... kDacut-off ﬁlter units. Full-length tACE protein was obtainedfollowing medium removal by washing the cells three timeswith NaCl ⁄ Pi and then scraping off with 1.5 mL of NaCl ⁄ Pi.For the chloride ... where extracellular chloride ion levels ﬂuctuate.Consequently, chloride regulation of ACE and ACE2could serve as a regulatory mechanism to maintain aphysiologically appropriate balance of activities.The ... ectodomain IgG (1 : 10 0) obtainedfrom R and D Systems Europe Ltd (Abingdon, UK) in 3%(w ⁄ v) bovine serum albumin in NaCl ⁄ Tris containing 0.1%(v ⁄ v) Tween 20 (TBST). After rinsing with...

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