báo cáo khoa học: " High levels of nucleotide diversity and fast decline of linkage disequilibrium in rye (Secale cereale L ) genes involved in frost response" doc
báo cáo khoa học: " High levels of nucleotide diversity and fast decline of linkage disequilibrium in rye (Secale cereale L.) genes involved in frost response" doc
... Access High levels of nucleotide diversity and fast decline of linkage disequilibrium in rye (Secale cereale L. ) genes involved in frost response Yongle Li 1 , Grit Haseneyer 1 , Chris-Carolin Schön 1 , ... well-suited for allele mining in genes involved in the frost responsive network. For investigating genetic diversity and the ext...
Ngày tải lên: 11/08/2014, 11:21
Báo cáo khoa học: High levels of structural disorder in scaffold proteins as exemplified by a novel neuronal protein, CASK-interactive protein1 pot
... several hundred proteins, including recep- tors and channels, cell adhesion proteins, cytoskeletal proteins, G-proteins and their modulators, and signal- ling molecules including kinases and phosphatases ... directly related to the involvement of disor- der in scaffold proteins. In fact, there is an elevated level of disorder in hub proteins, i.e. proteins involved in mu...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc
... were considerably higher. After purification and solubilization into detergent micelles, we could calculate a yield of resolubilizable protein of 1 mgÆmL )1 RM for YfiK, 1.5 mgÆmL )1 RM for SugE and of 2.7 ... GFP expression. L -a-phosphatidylcholine (LPC), 1,2-dimyris- toyl-sn-glycero-3-phosphocholine (DMPC), 1-palmitoyl-2- oleoyl-sn-glycero-3-phosphocholine (POGP) and an...
Ngày tải lên: 30/03/2014, 13:20
Báo cáo khoa học: " High-risk surgical stage 1 endometrial cancer: analysis of treatment outcome" pdf
... ( %) total ( %) Initial failure site 30 76 Vaginal 3 (1 0) 0 3 (2. 8) Pelvic 5 (1 7) 0 5 (4. 7) Distant 22 (7 3) 0 22 (20. 7) Alive 10 (7. 9) 70 (92. 1) 80 (75. 8) Outcome Patients relapses Yes No total ... < 0.000 1). In multivariate analyses the initial failure in distant sites (p < 0.000 1) was the only factor associated with poor survival. A...
Ngày tải lên: 09/08/2014, 10:21
Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... over-expressing subline (K562 ⁄ ADR), the GLC4 cell line and its MRP1 over-expressing subline (GLC4 ⁄ ADR), the 2008 cell line and its sublines over-expressing MRP1, MRP2 or MRP3, and the cell line HEK293 ... pumping of the agents out of the cells and their intracellular binding to receptors and ⁄ or pumping into intracellular compartments. Because the rhodamines were excluded f...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... on LB agar plates containing ampicillin (50 mg L )1 ), single colonies were picked for 2 mL over- night cultures in LB medium containing ampicillin (50 mg L )1 ), and plasmids were prepared using ... cells (BL21 DE3 PLysS Star) by heat shock and spread on LB agar plates containing ampicillin (50 mg L )1 ) and chlorampheni- col (30 mg L )1 ). Single colonie...
Ngày tải lên: 18/02/2014, 13:20
Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx
... is tightly linked to their thermodynamic stability. Methods for studying quadruplex folding and unfolding The folding and unfolding of G-quadruplexes can be conveniently monitored using spectroscopic ... Methods Enzymol 180, 304–325. 30 Mergny JL & Lacroix L (200 3) Analysis of thermal melting curves. Oligonucleotides 13, 515–537. 31 Mergny JL, Phan AT & Lacroix L (199...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-specific intronic inverted repeats pptx
... shock domain protein LIN-28 controls developmental timing in C. elegans and is regulated by the lin-4 RNA. Cell 88, 637–646. 18 Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, ... the high frequency of LIRs in long genes. A total of 956 LIRs (in 702 genes) were located in genic regions, and 1595 in intergenic regions. In other words, 37% of the LIRs wer...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... Z-Leu-Leu-Leucinal (Z-LLnL, MG13 2), N-benzyloxycarbonyl- L -leucinyl- L -leucinyl- L -leucinal; Z-Leu-Leu-Norvalinal (Z-LLnV, MG11 5), N-benzyloxycarbonyl- L -leucinyl- L -leucinyl- L -norvalinal. Enzyme: ... Biochem. 27 1) Ó FEBS 2004 was inhibited 12 h postinduction by addition of the commercially available inhibitors lactacystine, Z-Leu-Leu- Norvalinal or Z-Leu-Leu-Le...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identified by site-directed mutagenesis pot
... kDa cut-off filter units. Full-length tACE protein was obtained following medium removal by washing the cells three times with NaCl ⁄ P i and then scraping off with 1.5 mL of NaCl ⁄ P i . For the chloride ... where extracellular chloride ion levels fluctuate. Consequently, chloride regulation of ACE and ACE2 could serve as a regulatory mechanism to maintain a physiologically appropria...
Ngày tải lên: 16/03/2014, 04:20