báo cáo khoa học: " Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality" pot

... 11:1 http://www.biomedcentral.com/1471-2229/11/1 Page 5 of 14 RESEARCH ARTICLE Open Access Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality Sebastian ... 18:207-208. doi:10.1186/1471-2229-11-1 Cite this article as: Worch et al.: Haplotyping, linkage mapping and expressi...

Ngày tải lên: 11/08/2014, 11:21

... tissue expression patterns of the fabp3 genes from mammals and zebrafish were a result of evolutional diver- gence of transcriptional regulation. Materials and methods Growth and maintenance of zebrafish ... cDNA by 3¢ RACE. Agarose gel electrophoretic separation of 3¢ RACE products for zebrafish FABP3 cDNA cloning. Both the major band of  650 bp and the minor band of...

Ngày tải lên: 31/03/2014, 07:20

... linear isoform of the HNE peptide. Although the binding pattern may be somewhat blurred by these antibodies and by the polyclonal nature of the sera, the importance of residues C386, G388, Q391 and C394 ... partially oxidized and the signal of the reduced species increased as a result of oxidation. Identification of the active isoform Because of disulfide scrambling HPLC...

Ngày tải lên: 08/03/2014, 08:20

... ligand and within the ligand, and internal torsion energy for the ligand. To consider Fig. 2. Summary of sequential and medium-range NOE connectivi- ties observed for the major conformer of peptide ... Ramachandran plot analysis. AB N C C N A1 C2 L3 W5 S6 D7 P9 C10 P4 G8 Fig. 3. Superimposition of 50 structures (A) and ribbon diagram of the lowest energy structure of the m...

Ngày tải lên: 16/03/2014, 23:20

... procedures Cloning, expression and purification of recombinant proteins HAKN1 homeodomain and C -terminal sequences were amplified and cloned in-frame into the EcoRI and PstI sites of the expression vector ... state. Indeed, over- expression of some class I genes in Arabidopsis and tobacco produces the proliferation of meristems on the surface of leaves. The protein...

Ngày tải lên: 23/03/2014, 13:20

... concentrations of 300 lm, 300 lm and 500 lm, respectively. Cloning of the chicken MT cDNA and its separate a and b domains for recombinant expression The ckMT coding sequence, kindly provided by Dr G.K. Andrews ... in a total volume of 100 lL, comprising 2 lLof25mm dNTP mixture, 2 lLof20lm primer solution, 1 U of DeepVent DNA polymerase (New England Biolabs, Hitchin, England...

Ngày tải lên: 30/03/2014, 11:20

... particularly among the down -regulated genes. Expression of herbicide -regulated genes The time-dependent expression of 13 genes that were up -regulated by exposure to herbicide and five that A. Tanguy ... regulation of specific herbicide- regulated gene expression. Results Identification of herbicide -regulated genes Two forward and reverse SSH libraries were ma...

Ngày tải lên: 30/03/2014, 15:20

... J., PLIN and M6PRBP1 genes in pig 223 Figure 2a. Expression of PLIN, ADRP and M6PRBP1 mRNAs in porcine tissues. RT- PCR results for porcine PLIN, ADRP, M6PRBP1 and β-actin mRNAs. PCR of PLIN, ADRP and ... cycles and that of β-actin 28 cycles. Figure 2b. Comparison of mRNA levels of PLIN, ADRP and M6PRBP1 in various tissues. The level of β-actin mRNA was used to nor...

Ngày tải lên: 14/08/2014, 13:22

... alleviation of this inhibition by another sub- strate of the enzyme, ATP, are presented [8,9]. Results Solution properties of the N -terminal domain of PS, the C -terminal domain of PS, and full-length PS Full-length ... the structures of PSs from E. coli and M. tuberculosis. Structural analyses Analysis of the sites of substrate binding from NMR data, and structur...

Ngày tải lên: 16/02/2014, 09:20

... 7.0 and 4 °C for 6 h, followed by SDS ⁄ PAGE. The rate of ligation was estimated from percentage intensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and ... time-dependent enhance- ment of the 57-kDa band with a concomitant diminu- tion of the 38-kDa and 19-kDa bands, as analyzed by SDS ⁄ PAGE (Fig. 3A). The 38-kDa and 19-kDa b...

Ngày tải lên: 19/02/2014, 07:20