báo cáo khoa học: " Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality" pot

... 11:1http://www.biomedcentral.com/1471-2229/11/1Page 5 of 14RESEARCH ARTICLE Open Access Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed qualitySebastian ... 18:207-208.doi:10.1186/1471-2229-11-1Cite this article as: Worch et al.: Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality. BMC Plant Biology 2011 ... HA11J15u_s_at: AP2/EREBP family+3.0Figure 3 Expression profile s of mapped barley genes up -regulated by drought stress. Upper panel: hormone and signalling genes, lowerpanel: transcription factor...

Báo cáo khoa học: Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebraﬁsh (Danio rerio) pot

... tissue expression patterns of the fabp3 genes frommammals and zebraﬁsh were a result of evolutional diver-gence of transcriptional regulation.Materials and methodsGrowth and maintenance of zebraﬁsh ... cDNA by 3¢ RACE. Agarosegel electrophoretic separation of 3¢ RACE products for zebraﬁshFABP3 cDNA cloning. Both the major band of  650 bp and theminor band of  570 bp were excised, cloned and ... Amajor band of approximately 200 bp, and two minor bands of 250 bp and 180 bp, were observed after agarose gelelectrophoresis of nested PCR products from the CIP/TAP-treated RNA. No speciﬁc band...

Báo cáo khoa học: Functional ﬁne-mapping and molecular modeling of a conserved loop epitope of the measles virus hemagglutinin protein pdf

... linear isoform of the HNEpeptide. Although the binding pattern may be somewhatblurred by these antibodies and by the polyclonal nature of the sera, the importance of residues C386, G388, Q391 and C394 ... partially oxidized and thesignal of the reduced species increased as a result of oxidation.Identiﬁcation of the active isoformBecause of disulﬁde scrambling HPLC-puriﬁed isoformsrapidly re-equilibrate, ... epitope.Ó FEBS 2003 Fine -mapping of an epitope of measles virus (Eur. J. Biochem. 270) 1523Functional ﬁne -mapping and molecular modeling of a conservedloop epitope of the measles virus hemagglutinin...

Báo cáo khoa học: Structure, epitope mapping, and docking simulation of a gibberellin mimic peptide as a peptidyl mimotope for a hydrophobic ligand pot

... ligand and within the ligand, and internal torsion energy for the ligand. To considerFig. 2. Summary of sequential and medium-range NOE connectivi-ties observed for the major conformer of peptide ... Ramachandran plot analysis. ABNCCNA1C2L3W5S6D7P9C10P4G8Fig. 3. Superimposition of 50 structures (A) and ribbon diagram of the lowest energy structure of the major conformer of peptide ... thetertiary structures of the two minor conformers of pep-tide SD.Conformation of the major conformer of peptideSDThe 3D structure of the major conformer of peptideSD was determined by simulated annealing...

Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunﬂower KNOX protein HAKN1 with DNA ppt

... proceduresCloning, expression and puriﬁcation of recombinant proteinsHAKN1 homeodomain and C -terminal sequences wereampliﬁed and cloned in-frame into the EcoRI and PstI sites of the expression vector ... state. Indeed, over- expression of some class I genes in Arabidopsis and tobacco produces the proliferation of meristems on thesurface of leaves.The proteins encoded by knox genes belong to thethree-amino-acid ... this class of genes constitutes a family present throughout the plant king-dom. The knox family of genes can be subdivided intotwo classes, I and II, by sequence relatedness and expression...

Báo cáo khoa học: Comparative metal binding and genomic analysis of the avian (chicken) and mammalian metallothionein potx

... concentrations of 300 lm, 300 lm and 500 lm, respectively.Cloning of the chicken MT cDNA and its separatea and b domains for recombinant expression The ckMT coding sequence, kindly provided by Dr G.K.Andrews ... in a total volume of 100 lL,comprising 2 lLof25mm dNTP mixture, 2 lLof20lmprimer solution, 1 U of DeepVent DNA polymerase (NewEngland Biolabs, Hitchin, England) and 100 ng of the tem-plate ... accomplished by determination of the spectroscopic and spectrometric features of theZn-, Cd- and Cu- complexes rendered by the recom-binant full-length protein and its separate b and adomains,...

Báo cáo khoa học: Molecular identiﬁcation and expression study of differentially regulated genes in the Paciﬁc oyster Crassostrea gigas in response to pesticide exposure doc

... particularly amongthe down -regulated genes. Expression of herbicide -regulated genes The time-dependent expression of 13 genes that wereup -regulated by exposure to herbicide and ﬁve thatA. Tanguy ... regulation of speciﬁc herbicide- regulated gene expression. ResultsIdentiﬁcation of herbicide -regulated genes Two forward and reverse SSH libraries were madefrom pooled digestive glands and gills of ... – +RiboL13 ADI (down) + + + +Fig. 1. Analysis of differential expression of up- and down -regulated genes in C. gigas exposed to pesticides. Expression of the gene studiedis presented as the...

Báo cáo sinh học: "Cloning, chromosome mapping and expression pattern of porcine PLIN and M6PRBP1 genes" pdf

... J.,PLIN and M6PRBP1 genes in pig 223Figure 2a. Expression of PLIN, ADRP and M6PRBP1 mRNAs in porcine tissues. RT-PCR results for porcine PLIN, ADRP, M6PRBP1 and β-actin mRNAs. PCR of PLIN,ADRP and ... cycles and that of β-actin 28 cycles.Figure 2b. Comparison of mRNA levels of PLIN, ADRP and M6PRBP1 in varioustissues. The level of β-actin mRNA was used to normalize those of PLIN, ADRP and M6PRBP1. ... droplets and are involved in the metabolism of intracellular lipid. This work describes the cloning and sequencing of porcine PLIN and M6PRBP1 cDNAs, the chromosome mapping of these two genes, ...

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... alleviation of this inhibition by another sub-strate of the enzyme, ATP, are presented [8,9].ResultsSolution properties of the N -terminal domain of PS, the C -terminal domain of PS, and full-lengthPSFull-length ... thestructures of PSs from E. coli and M. tuberculosis.Structural analyses Analysis of the sites of substrate binding from NMR data, and structural comparison of the E. coli, M. tuberculosis and A. ... 164° and )169° in the A-chain and B-chain of nPS, respectively. The v1torsion angle forAsp35 changes from ) 75.20° to )171.65° and )89.25°in the A-chain and B-chain of nPS, and in the case of His37,...

Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx

... 7.0 and 4 °C for 6 h, followed by SDS ⁄ PAGE. The rate of ligation was estimated from percentageintensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and ... time-dependent enhance-ment of the 57-kDa band with a concomitant diminu-tion of the 38-kDa and 19-kDa bands, as analyzed by SDS ⁄ PAGE (Fig. 3A). The 38-kDa and 19-kDa bandsdid not disappear completely ... protein was identiﬁed by SDS ⁄ PAGE [21] and further conﬁrmed by analyzing the N- terminal amino-acid sequence [15] by automated Edmandegradation after electroblotting of protein bands inSDS ⁄ polyacrylamide...

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