báo cáo khoa học: " Conjugated polymer nanoparticles for effective siRNA delivery to tobacco BY-2 protoplasts" potx

... 10:291 http://www.biomedcentral.com/1471-2229/10/291 Page 11 of 14 METH O D O LOG Y AR T I C LE Open Access Conjugated polymer nanoparticles for effective siRNA delivery to tobacco BY-2 protoplasts Asitha T Silva 1 , Alien Nguyen 2 , Changming ... 7.4). For siRNA delivery experiments, protoplasts were cultured in cell wall regeneration medium. siRNA delivery to...

Ngày tải lên: 11/08/2014, 11:21

... induced for 3 min by addition of 1 mm IPTG for pEH vectors and 0.2 lgÆmL )1 anhydrotetracycline for pASK-IBA vectors, fol- lowed by pulse labelling with [ 35 S]methionine (30 lCiÆmL )1 ) for 2 ... SDS–PAGE due to the added molecular mass of AMS (0.5 kDa). In control samples, cells were lysed prior to AMS treatment to allow access to cysteines exposed in the cytoplasm. First we...

Ngày tải lên: 07/03/2014, 02:20

... application to Natural Language Database Interfacing. Ph.D. Thesis, Royal Institute of Technology, Stockholm, Sweden. 316 Semantic Information Preprocessing for Natural Language Interfaces to Databases ... predicates). Output is a logical formula, consist- ing of predicates meaningful to the database engine (database predicates). AET provides a formalism for describing how a...

Ngày tải lên: 08/03/2014, 07:20

... from the forest quickly enough and where the treatment capacities of the local forestry commission are limited. We carried out a number of experiments in the Forest Protection ... Protection Department of the Warsaw Forest Research Institute in 1986 and 1987. These concerned improvements to insecticide application methods for use in Scots pine w...

Ngày tải lên: 08/08/2014, 23:21

... an application to beef cattle data. employed as an effective tool to reduce the number of traits to be handled (and breeding values to be reported) for ’traits’ measured ... assumed to be the same for all ages. For each order of fit, the number of non-zero eigenvalues allowed for each coefficient matrix to be estimated was set t...

Ngày tải lên: 09/08/2014, 18:21

... significant), nausea (9% for remifentanil and 6% for fentanyl; not significant), fever (5% for remifentanil and 9% for fentanyl; not significant) and vomiting (5% for remifen- tanil and 6% for fentanyl; ... percentages for the extubation and pos- textubation phases, and the post-treatment period were 23% for remifentanil and 7% for fentanyl, 33% for remifentanil and 9% fo...

Ngày tải lên: 12/08/2014, 20:20

... ambiguous personal names (totally 109 personal names); and for each name, we need to disambiguate its observations in the web pages of the top N (100 for WePS1 and 150 for WePS2) Yahoo! search ... the link structure of Wikipedia Fortunately, in recent years, due to the evolu- tion of Web (e.g., the Web 2.0 and the Semantic Web) and many research efforts for the construc- tio...

Ngày tải lên: 16/03/2014, 23:20

... per group for t = 7, n = 3 to 7 rats per group for t = 10, n = 5 to 8 rats per group for t = 14, n = 5 to 9 rats per group for t = 21, and n = 4 to 6 rats per group for t = 35. *P < 0.05 compared ... in AA was enriched for activated auto-aggressive CD4 + T cells, as shown by the specific response to the disease- associated epitope Mt HSP60 176–190 . Specific targetin...

Ngày tải lên: 09/08/2014, 06:22

... concerns for the safety of applied nanoparticles for personal care and cosmetics, since these agents will presumably increase the penetration potential of nanoparticles. As such, the cytotoxicity of nanoparticles ... used for testing the toxicity and trafficking of nanoparticles [33-35]. In the experimental study, we incubated 1 μg/ml ivy nano- particles with HeLa cells for 24...

Ngày tải lên: 11/08/2014, 00:22

... distance setting for the AFM used in this study was only 3 um, it was not possible to generate force curves from t he fresh liquid adhesive due to its high elasticity. Instead, we chose to study the ... ratio of polysaccharide to water has proven to be crucial in the formation of the unique elastic proper- ties of the adhesive, as seen with other polymers [13-17]. Due to the dif...

Ngày tải lên: 11/08/2014, 00:22