báo cáo khoa học: "Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L )" doc
báo cáo khoa học: "Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.)" doc
... Fully formattedPDF and full text (HTML) versions will be made available soon.Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L. )BMC ... 1Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L. ). Matthew W. Blair1*, Andrea C. Fernandez1, Manabu Ishitani1, Danilo Moreta1, ... synthesis of even longer full-length cDNAs, and higher representation of long full-length cDNAs in the library. In summary, this method of selecting full-length cDNAs by biotinylation of the mRNA...
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Báo cáo khoa học: Construction and biological activity of a full-length molecular clone of human Torque teno virus (TTV) genotype 6 pptx
... tool for exploring theviral determinants important in virus–cell interactions, and to find permissive cell lines for further molecular and cell biological studies of this peculiar human virus. For ... The resultswere identical for all the cell lines.Replication of TTV DNAAll seven cell lines were transfected with linTTV and with the intact pTTV. For detection of TTV replication,total DNA ... replication. The replicating full-length clone, the cell linesfound to support TTV replication, and the methods presented here willfacilitate the elucidation of the molecular biology and the life...
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báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps
... a sample of 250 μlwastakenattheendofeach hour for SDS-PAGE analysis.Cell lysis and protein extractionBacterial cells were harvested by centrifugation of cellculture at 4000 rpm, 4°C for 10 ... Institute for financialsupport and declare no conflict of interest in this research work. We alsoappreciate all our colleagues listed in the references for proving invaluableinformation which helped ... convenience for scaling up and automation [9-18]. In these researchreports and a lso commercially available manuals, littleinformation is available on their performance, and it isoften quoted...
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Báo cáo khoa hoc:" Construction and characterization of a bovine BAC library with four genome-equivalent coverage" pptx
... DH10B cells [9]were harvested from 200 mL of mid-log phase cultures grown in SOB medium,washed twice in ice-cold water with the same volume of culture and once in50 mL. Finally, cells were ... mixing respectively 300 L and 3 times 120 L of the bacteria culture. Thedifferent pools were collected manually. For each pool, bacteria were thenpelleted, resuspended in TE, and the DNA was ... three copies of each 96-well microtitre platewere prepared and stored at −80◦C at different locations. Superpools of 24plates and pools of individual plates (24), 12 columns and 8 rows were...
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báo cáo khoa học: " Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset" pptx
... translation levels of hundreds of genes[35,36]. These acute molecular changes are related toplant cell physiological and biochemical modifications(cold acclimation) that lead to stress tolerance ... shelf-life at 20°C for 2-6 days (E4: Ripe; long term cold storage; woolly fruits). The expression level of Pplox1 was analyzed as a control for genes that do not express preferentially in cold ... conditions for this first round of amplifi-cations was: 1 cycle at 93°C for 10 min, 7 cycles of 93°C for 30 sec, 72°C for 15 min, followed by 37 cycles of 93°C for 30 sec, 67°C for 15 min. For the nested...
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Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
... detail by using X-ray crystallography.Three-dimensional structures of RNase AÆN-acyl-sulfonamide-linked nucleoside complexesThe three-dimensional structures of N-acylsulfona-mides 6 and 7 ... (1991) Buildingblocks for oligonucleotide analogs with dimethylenesulfide, sulfoxide, and sulfone groups replacingphosphodiester linkages. J Org Chem 56, 3869–3882.21 Musicki B & Widlanski ... December2010)doi:10.1111/j.1742-4658.2010.07976.xMolecular probes are useful for both studying and controlling the functions of enzymes and other proteins. The most useful probes have high affinity for their target, along with small size and...
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Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc
... immunoblotting as cN-IIbut completely inactive. E. coli produces a smallamount of cN-II that is correctly folded and a largeamount of incorrectly folded and insoluble protein. Itis conceivable ... members of the haloacid dehalogenase (HAD) superfamily,including the soluble 5¢-nucleotidase family: cytosolic5¢-nucleotidases cN-II, cN-III, cN-IA and cN-IB, and both cytosolic and mitochondrial ... structure of the whole protein. Atruncated form of cN-II lacking the last 25 aminoacids is significantly more soluble than the wild-typeenzyme, and was recently crystallized [11]. The crystal-lographic...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... high-throughput crystallography of protein-ligand complexes. Acta Crystallogr D Biol Crystallogr60, 1355–1363.38 DeLano WL (2002) The PyMOL Molecular GraphicsSystem. DeLano Scientific, Palo Alto.39 Chenna ... coliBL21(DE3) strain and stored at )80 °C for further use.One hundred and fifty milliliters of LB supplemented with50 lgÆmL)1kanamycin was inoculated and grown at 37 °Covernight; 40 mL of ... this culture were used to inoculate 3 L of TB supplemented with 50 lgÆmL)1kanamycin and approximately 50 lL of Breox antifoam (Cognis Perfor-mance Chemical UK Ltd) in glass flasks using the largescale...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... whole-cell conversion of d ,l- p-HPH was alsoWTF63CF63DF63EF63GF63HF63IF63KF6 3L F63MF63NF63PF63QF63RF63SF63TF63VF63WF63YWT L9 2C L9 2D L9 2E L9 2F L9 2G L9 2H L9 2I L9 2K L9 2M L9 2N L9 2P L9 2Q L9 2R L9 2S L9 2T L9 2V L9 2W L9 2YWTF150CF150DF150EF150GF150HF150IF150KF15 0L F150MF150NF150PF150QF150RF150SF150TF150VF150WF150Y1201008060Relative ... isopropyl-b-d-thiogalacto-pyranoside at a final concentration of 1 mm.Bacterial strains and culture conditionsE. coli strain DH5a was used for the cloning and amplifica-tion of all constructed plasmids. ... smaller hydrophobicresidues, actually reduced the catalytic activity to lessthan approximately 50% of that of the wild-typeenzyme. In addition, replacement of Leu92 by Ile orPhe had a negligible...
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Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain pdf
... package for modelling the dynamics, steady states and control of biochemical and other systems. Comput Appl Biosci 9,563–571.67 Noble MA, Munro AW, Rivers SL, Robledo L, DaffSN, Yellowlees LJ, ... detail. An interesting and possibly novel connectionappears to link regulation of KeqA by the CT and Table 1. Factors that may alter conformational equilibrium A, B and ⁄ or the rate of electron ... peptide, Garcin et al. [22] constructed a model for full-length nNOS that indicates that an allowablelarge motion of the FMN module could bring theFMN cofactor within an acceptable electron-transferdistance...
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