báo cáo khoa học: "Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L )" doc

... Fully formatted PDF and full text (HTML) versions will be made available soon. Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L. ) BMC ... 1 Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L. )....

Ngày tải lên: 11/08/2014, 11:21

... tool for exploring the viral determinants important in virus–cell interactions, and to find permissive cell lines for further molecular and cell biological studies of this peculiar human virus. For ... The results were identical for all the cell lines. Replication of TTV DNA All seven cell lines were transfected with linTTV and with the intact pTTV. For detection of TTV r...

Ngày tải lên: 16/03/2014, 05:20

... a sample of 250 μlwastakenattheendof each hour for SDS-PAGE analysis. Cell lysis and protein extraction Bacterial cells were harvested by centrifugation of cell culture at 4000 rpm, 4°C for 10 ... Institute for financial support and declare no conflict of interest in this research work. We also appreciate all our colleagues listed in the references for proving invaluable inf...

Ngày tải lên: 11/08/2014, 00:23

... DH10B cells [9] were harvested from 200 mL of mid-log phase cultures grown in SOB medium, washed twice in ice-cold water with the same volume of culture and once in 50 mL. Finally, cells were ... mixing respectively 300 L and 3 times 120 L of the bacteria culture. The different pools were collected manually. For each pool, bacteria were then pelleted, resuspended in TE, and th...

Ngày tải lên: 09/08/2014, 18:21

... translation levels of hundreds of genes [35,36]. These acute molecular changes are related to plant cell physiological and biochemical modifications (cold acclimation) that lead to stress tolerance ... shelf-life at 20°C for 2-6 days (E4: Ripe; long term cold storage; woolly fruits). The expression level of Pplox1 was analyzed as a control for genes that do not express prefere...

Ngày tải lên: 12/08/2014, 03:21

... detail by using X-ray crystallography. Three-dimensional structures of RNase AÆN-acyl- sulfonamide-linked nucleoside complexes The three-dimensional structures of N-acylsulfona- mides 6 and 7 ... (1991) Building blocks for oligonucleotide analogs with dimethylene sulfide, sulfoxide, and sulfone groups replacing phosphodiester linkages. J Org Chem 56 , 3869–3882. 21 Musicki B & Wi...

Ngày tải lên: 14/02/2014, 22:20

... immunoblotting as cN-II but completely inactive. E. coli produces a small amount of cN-II that is correctly folded and a large amount of incorrectly folded and insoluble protein. It is conceivable ... members of the haloacid dehalogenase (HAD) superfamily, including the soluble 5¢-nucleotidase family: cytosolic 5¢-nucleotidases cN-II, cN-III, cN-IA and cN-IB, and both cytosolic...

Ngày tải lên: 15/02/2014, 01:20

... high-throughput crystallography of protein- ligand complexes. Acta Crystallogr D Biol Crystallogr 60, 1355–1363. 38 DeLano WL (2002) The PyMOL Molecular Graphics System. DeLano Scientific, Palo Alto. 39 Chenna ... coli BL21(DE3) strain and stored at )80 °C for further use. One hundred and fifty milliliters of LB supplemented with 50 lgÆmL )1 kanamycin was inoculated and grown at 37...

Ngày tải lên: 15/02/2014, 01:20

... whole-cell conversion of d ,l- p-HPH was also WT F63C F63D F63E F63G F63H F63I F63K F6 3L F63M F63N F63P F63Q F63R F63S F63T F63V F63W F63Y WT L9 2C L9 2D L9 2E L9 2F L9 2G L9 2H L9 2I L9 2K L9 2M L9 2N L9 2P L9 2Q L9 2R L9 2S L9 2T L9 2V L9 2W L9 2Y WT F150C F150D F150E F150G F150H F150I F150K F15 0L F150M F150N F150P F150Q F150R F150S F150T F150V F150W F150Y 120 100 80...

Ngày tải lên: 18/02/2014, 08:20

... package for modelling the dynamics, steady states and control of biochemical and other systems. Comput Appl Biosci 9, 563–571. 67 Noble MA, Munro AW, Rivers SL, Robledo L, Daff SN, Yellowlees LJ, ... detail. An interesting and possibly novel connection appears to link regulation of K eq A by the CT and Table 1. Factors that may alter conformational equilibrium A, B and ⁄ or t...

Ngày tải lên: 18/02/2014, 11:20