báo cáo khoa học: "Characterization of the stress associated microRNAs in Glycine max by deep sequencing" pot

... while they only account for 15.69% in the alkalinity induced library. The relatively lower percentage of 24 nt reads indicates that more kinds of miRNAs are involved in the response of G. max ... were filtered and the remaining tags were aligned with the Glycine max genome. To analyze whether the matched sequence could form a suitable hairpin (the secondary s...

Ngày tải lên: 11/08/2014, 11:21

... to the ribosome by increasing the magnesium concentration [14]. The magnesium concentration also affects the binding states of the tRNAs on the ribosome. Unacylated tRNAs can be bound in either ... retained on the filter in the presence of SF (Table 2). The effect was specific to SF, as incu- bation of unacylated tRNA with recombinant ribo- somal protein L10 or ova...

Ngày tải lên: 07/03/2014, 16:20

... pH or by FC-induced binding of 14-3-3s. Results The C-terminus of isoform 1 of the PM H + -ATPase of A. thaliana (AHA1) interacts with the first 88 amino acids of PPI1 [22], indicating that the ... residue of the PM H + -ATP- ase results in binding of 14-3-3 protein. 14-3-3 binding is stabilized by the fungal toxin FC which decreases the dissociation rate, thu...

Ngày tải lên: 19/02/2014, 07:20

... renaturation of either one or both of the proteins was incomplete and the complex formation is slow and/or incomplete under the conditions employed. Inhibition of the glutaminase activity by DON Glutamine ... Sequencing of the mutated genes revealed that there was indeed a mutation on both of the genes. In SNO1, the 199th G from the 5¢ terminus of the open re...

Ngày tải lên: 19/02/2014, 12:20

... binding factors. The introduction of mutation in one of the Ets-binding sequences greatly decreased its promoter activity, suggesting that the transcription of the a3 integrin gene in these cells ... including Ets, GATA, and MyoD/E-box binding factors. The introduction of mutation into one of the putative Ets- binding sequences suppressed the promoter activity. In...

Ngày tải lên: 21/02/2014, 03:20

... suggested a single cut at the C9–C10 double bond of the ring-bearing side of 3-OH-c-carotene in combination with several cleavage options in the linear half of the molecule, namely at the C9¢–C10¢,C7¢–C8¢ ... dialdehydes on the basis of the fine structure of the corresponding UV–visible spectra. The three dialdehydes differed in their chain lengths, as indicat...

Ngày tải lên: 07/03/2014, 03:20

... arrows). Owing to the horizontal smear, however, the presence of the B14 subunit in the lower band cannot be ascer- tained at this point. A cross-reaction of the antibody against the 39 kDa subunit of ... piericidin are speci- fic inhibitors of complex I in eukaryotes, we assume that they bind to the large form of the complex, which contains a homolog of the B...

Ngày tải lên: 07/03/2014, 09:20

... space beside the absence of c-turn minima. Discussion The nonsubstituted proline analogue [Pro11]SP is only a weak competitor of SP binding sites. Reintroducing the methionine side chain in the pyrrolidine ... structure. The confor- mations of residues 8–9 are ill defined in the peptides. Thus, the introduction of a prolinoamino acid in the C-terminal tail of SP...

Ngày tải lên: 08/03/2014, 02:21

... nonheated samples, the mature form of GluV8 could be renatured even after exposure to heat in the presence of SDS. Role of N-terminal Val69 in processing of the GluV8 proform Finally, the role of N-terminal ... for the correct folding of GluV8, indicating its role as an intramolecular chap- erone. Furthermore, the four amino acid residues at the C-terminus of...

Ngày tải lên: 16/03/2014, 06:20

... of buffer A. The column was then washed with further 10 column volumes of buf- fer A, and thereafter the bound proteins were eluted by applying a linear gradient of 0–0.6 mm NaCl in a total of 40 ... to occur in their natural environment. This argument is also substantiated by the finding that bga1 is induced by d-galactose and l-arabinose, thus pointing to a role for...

Ngày tải lên: 16/03/2014, 11:20