báo cáo khoa học: " Characterization of highly efficient heavy-ion mutagenesis in Arabidopsis thaliana" pdf

... of the six breakpoints of rearrangements had deletions, whereas no breakpoint contained a duplication (Figure 3). A previous study revealed that the breakpoints induced by C ions with LET of ... approach, called Targeted Induced Local Lesions in Genomes (TILLING) [5-9]. Because of its mutation-inducing property, EMS is also very useful for producing leaky alleles in forward ge...

Ngày tải lên: 11/08/2014, 11:21

... (TOF-TOF MS) allowed us to investigate the N-terminal processing of CGA in rat heart extracts. Our data reveal the pres- ence of several vasostatin I-containing and vasosta- tin II-containing ... angiotensin II. In the heart, an- giotensin converting enzyme and ⁄ or renin, which are present on cell membranes, are involved in the conver- sion of angiotensinogen into angiotensin II...

Ngày tải lên: 07/03/2014, 12:20

... cell-type alkaline phosphatase; hPLAP, human placental alkaline phosphatase; IAP, intestinal alkaline phosphatase; M1, metal-binding site 1; M2, metal-binding site 2; M3, metal-binding site 3; PLAP, ... the presence of a triad of zinc atoms at the active site of rIAP-I, but not rIAP-II or hPLAP. Although no differences in amino acid alignment in the vicinity of metal-binding site...

Ngày tải lên: 07/03/2014, 17:20

... Adjustments of the cell fractionation proto- col involved reducing the starting material and the volume of the sucrose layers but maintaining the relative ratios of the sucrose layers. Infected ... glycosy- lation of the native site in the S1–S2 linker was required for proper trafficking and stability [43]. On the other hand, glycosylation of the native site in the S1–S2 linker...

Ngày tải lên: 16/03/2014, 13:20

... for 15.69% in the alkalinity induced library. The relatively lower percentage of 24 nt reads indicates that more kinds of miRNAs are involved in the response of G. max to alkalinity compared ... applications of high-throughput sequencing technologies are arousing much research interest, such as identification of entire sets of miRNAs, which deliver new insights into the ro...

Ngày tải lên: 11/08/2014, 11:21

... zinc-finger-like motifs. The single zinc finger motif of a small subset of WRKY proteins is distinct from that of group 1 and 2 members. Instead of a C 2 H 2 pattern, their WRKY domains contain a C 2 HC motif. ... 1). Clustering of OsWRKY genes according to their expression profile in the NIAS 22 K arrayFigure 3 Clustering of OsWRKY genes according to their expression profile...

Ngày tải lên: 12/08/2014, 03:21

... although the starting intensity values and values at the minimum were different, reflecting different pools of ML inside the cells. By changing the seeding density, the minimum of the curve was ... FEBS where the relevant increase of m 2 produces the relevant intensity changes of ML ⁄ TG signals. In principle, such an increase can be due to both a decrease in c 1 and an increase i...

Ngày tải lên: 18/02/2014, 13:20

... sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence. Cloning and sequencing of this fragment revealed an ORF showing amino acid ... by incubating sections in 0.3% hydrogen peroxide in methanol for 30 min at room temperature. Slides were then washed and incubated in a blocking solution accord- ing to the...

Ngày tải lên: 19/02/2014, 00:20

... lines indicate intron 6 of 75 bp in fragments B and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and 3¢ portion of 25 bp in intron 6 in fragments F and I. The number at the right of ... results in no generation of protein isoforms. The 5¢-UTR of eukaryotic mRNA in uences the initi- ation step of protein synthesis and thereby in part determines the tran...

Ngày tải lên: 19/02/2014, 06:20

... C-terminus of isoform 1 of the PM H + -ATPase of A. thaliana (AHA1) interacts with the first 88 amino acids of PPI1 [22], indicating that the PM H + -ATPase binding site of PPI1 is localized therein. ... results in binding of 14-3-3 protein. 14-3-3 binding is stabilized by the fungal toxin FC which decreases the dissociation rate, thus inducing the formation of an almost irreve...

Ngày tải lên: 19/02/2014, 07:20