báo cáo khoa học: " Conservation and Loss of Ribosomal RNA Gene Sites in Diploid and Polyploid Fragaria (Rosaceae)" doc

... hybridization signals, among and within homologous sites could be explained by such events. Conservation and Loss of Ribosomal RNA Gene Sites in Diploid and Polyploid Fragaria (Rosaceae) Bo Liu, ... evolution of ribosomal RNA genes during polyploidization in Fragaria. Results: Root tip cells of diploid taxa were typified by two 5S and six 25S rD...

Ngày tải lên: 11/08/2014, 11:21

... of interest for endogenous generation of siRNA and activation of the RNAi effect in human cells [45]. Studies on an inhibition of BACE gene expression by endogenously generated siRNA via tRNA Val promotion ... decomposition of the RNA. Determination of the level of BACE mRNA and ribozyme RNA expression in mammalian cells The level of BACE mRNA and ribozyme RNA...

Ngày tải lên: 08/03/2014, 08:20

... elongation of the prenyl chain of the final product. In addition, two amino acids inserted into FARM, which occurs in FPSs and GGPSs from bacteria and plants, are also considered to be involved in the ... while smaller amino acid residues such as valine, alanine, serine, cysteine or glutamic acid are conserved in the longer-chain (all-E) prenyl diphosphate synthases and in...

Ngày tải lên: 23/03/2014, 17:21

... one of the RNA binding sites of Kid and could also be responsible for the distortion of the second RNA binding site by opening the N-termi- nal hairpin between b-strands 1 and 2 (Fig. 3C). Genetic ... region of Kid. Binding of this ligand clearly inhibited the RNase activity of Kid [25]. Native MS using the uncleavable RNA mimetic indicates that a dimer of the Kid to...

Ngày tải lên: 18/02/2014, 04:20

... validated in vitro andusedtoexamine the branch-point kinetics in detail and to obtain insights into the kinetic controls of methionine and threonine synthesis in plants. The branch-point between ... divergence of two fluxes (fluxes of cystathionine and threonine) with the convergence of two fluxes (fluxes of Phser and cysteine). Interestingly, the properties of CGS are s...

Ngày tải lên: 20/02/2014, 02:21

... (Oxis International Inc, Portland, OR, USA), follow- ing the manufacturer’s instructions. Culture of THP-1 cells and experimental protocol THP-1 monocytes were maintained in suspension in RPMI ... remnants of triglyceride-rich lipoproteins. Arterioscler Thromb 14, 622–635. 14 Pal S, Semorine K, Watts GF & Mamo J (2003) Identi- fication of lipoproteins of intestinal origin in...

Ngày tải lên: 16/03/2014, 12:20

... con- tains 24 EBNA-1 binding sites and a 65 base dyad symmetry element [22], can function as a terminator region of RNA polymerase II. Close inspection revealed that the mRNA of OCT1h was predominantly terminated ... predominantly terminated upstream of the EBNA-1 binding site region. With pEBTetD, the copy number of OCT1h mRNA was reduced as compared with pEBTet by a factor of...

Ngày tải lên: 23/03/2014, 09:21

... the substrate-binding tunnel in 1CEL, which are the d eterminants of t he glycosyl- binding sites , are conserved in 1Q9H. Density w as poor for one of the tunnel-forming loops of 1Q9H (residues ... amilies, of which 43 have b een assigned a retaining mechanism of action, 24 an inverting mechanism, and the stereochemical mode of action of the remaining families have yet...

Ngày tải lên: 23/03/2014, 13:20

... catalysis of isolated CF 1. The N-terminal region of subunit c in CF 1 is not crucial for binding of subunit e in vitro. Further studies are needed to determine candidate residues participating in transmission ... hydrolytic activity. In vitro binding assays of e with c subunit and immunoblotting analysis Equal amounts of GST or GST–e fusion proteins were bound to glutathi...

Ngày tải lên: 23/03/2014, 13:20

... components which are involved in the pathogenesis in bovine, ovine, and caprine mastitis [29]. In cows, intramammary infection (IMI) due to S. aureus is generally subclinical. Nevertheless, IMI causes considerable ... of capsule by the organism and the autoagglutination in the presence of low concentrations of sodium hydroxide. Although the addition of milk whey in some...

Ngày tải lên: 07/08/2014, 14:23