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báo cáo khoa học: " Conservation and Loss of Ribosomal RNA Gene Sites in Diploid and Polyploid Fragaria (Rosaceae)" doc

báo cáo khoa học:

báo cáo khoa học: " Conservation and Loss of Ribosomal RNA Gene Sites in Diploid and Polyploid Fragaria (Rosaceae)" doc

... hybridization signals, among and within homologous sites could be explained by such events. Conservation and Loss of Ribosomal RNA Gene Sites in Diploid and Polyploid Fragaria (Rosaceae) Bo Liu, ... evolution of ribosomal RNA genes during polyploidization in Fragaria. Results: Root tip cells of diploid taxa were typified by two 5S and six 25S rDNA hybridization signals of varying intensities, ... acceptance. Fully formattedPDF and full text (HTML) versions will be made available soon. Conservation and Loss of Ribosomal RNA Gene Sites in Diploid and Polyploid Fragaria (Rosaceae)BMC Plant...
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Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc

Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc

... of interest forendogenous generation of siRNA and activation of theRNAi effect in human cells [45]. Studies on an inhibition of BACE gene expression by endogenously generatedsiRNA via tRNAValpromotion ... decomposition of the RNA. Determination of the level of BACE mRNA and ribozyme RNA expression in mammalian cellsThe level of BACE mRNA and ribozyme RNA wasmonitored by RT/PCR using a OneStep ... preclearing process of thecell protein extract (100 lg of protein in 500 lLofextract) was carried out as described above. Immuno-precipitation and determination of the intracellular level of Ab40...
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Báo cáo khoa học: An alternative mechanism of product chain-length determination in type III geranylgeranyl diphosphate synthase docx

Báo cáo khoa học: An alternative mechanism of product chain-length determination in type III geranylgeranyl diphosphate synthase docx

... elongation of the prenyl chain of the finalproduct. In addition, two amino acids inserted into FARM,which occurs in FPSs and GGPSs from bacteria and plants,are also considered to be involved in the ... whilesmaller amino acid residues such as valine, alanine, serine,cysteine or glutamic acid are conserved in the longer-chain(all-E) prenyl diphosphate synthases and in the short-chainenzymes of other ... the area, including FARM and severalamino acids upstream of it, as the CLD (chain-lengthdetermination) region and proposed a classification of theseenzymes based on the patterns of such characteristic...
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Tài liệu Báo cáo khoa học: parDtoxin–antitoxin system of plasmid R1 – basic contributions, biotechnological applications and relationships with closely-related toxin–antitoxin systems ppt

Tài liệu Báo cáo khoa học: parDtoxin–antitoxin system of plasmid R1 – basic contributions, biotechnological applications and relationships with closely-related toxin–antitoxin systems ppt

... one of the RNA binding sites of Kid and could also be responsible for the distortion of the second RNA binding site by opening the N-termi-nal hairpin between b-strands 1 and 2 (Fig. 3C).Genetic ... region of Kid. Binding of this ligand clearlyinhibited the RNase activity of Kid [25]. Native MSusing the uncleavable RNA mimetic indicates that adimer of the Kid toxin interacts with a single RNA substrate. ... Authors Journal compilation ª 2010 FEBSmonomers are involved in RNA binding, and disrup-tion of the orientation of the amino terminal hairpinby the C-terminal tail of the antitoxin inactivates...
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Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc

Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc

... validated in vitro andusedtoexaminethe branch-point kinetics in detail and to obtain insights intothe kinetic controls of methionine and threonine synthesis in plants.The branch-point between ... divergence of two fluxes (fluxes of cystathionine and threonine) with theconvergence of two fluxes (fluxes of Phser and cysteine).Interestingly, the properties of CGS are such that the flux of cystathionine ... variables and to explain thebehaviour of the branch-point in terms of the kineticproperties of CGS and TS.Sensitivity analysis In a first analysis, fluxes of cystathionine and threonine(Fig....
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Báo cáo khoa học: Fatty acid composition of chylomicron remnant-like particles influences their uptake and induction of lipid accumulation in macrophages pot

Báo cáo khoa học: Fatty acid composition of chylomicron remnant-like particles influences their uptake and induction of lipid accumulation in macrophages pot

... (Oxis International Inc, Portland, OR, USA), follow-ing the manufacturer’s instructions.Culture of THP-1 cells and experimental protocolTHP-1 monocytes were maintained in suspension in RPMI ... remnants of triglyceride-rich lipoproteins.Arterioscler Thromb 14, 622–635.14 Pal S, Semorine K, Watts GF & Mamo J (2003) Identi-fication of lipoproteins of intestinal origin in humanatherosclerotic ... Radioactivitywas determined by liquid-scintillation counting usingHydrofluor as the scintillant.Analytical methodsTC (cholesterol + cholesteryl ester) and TG in CRLPs and in cells was determined by enzymatic...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt

Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt

... con-tains 24 EBNA-1 binding sites and a 65 base dyadsymmetry element [22], can function as a terminatorregion of RNA polymerase II. Close inspectionrevealed that the mRNA of OCT1h was predominantlyterminated ... predominantlyterminated upstream of the EBNA-1 binding siteregion. With pEBTetD, the copy number of OCT1hmRNA was reduced as compared with pEBTet by afactor of nine in the on-state and by a factor of 26 in the ... establish-ment of doxycycline-inducible protein expression by just a single transfec-tion. pEBTetD contains an Epstein–Barr virus origin of replication forstable and efficient episomal propagation in human...
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Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt

Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt

... the substrate-bindingtunnel in 1CEL, which are the d eterminants of t he glycosyl-binding sites , are conserved in 1Q9H. Density w as poor forone of the tunnel-forming loops of 1Q9H (residues ... amilies, of which 43 have b een assigneda retaining mechanism of action, 24 an invertingmechanism, and the stereochemical mode of action of the remaining families have yet to b e determined. Theendoglucanases ... inducer in T. emersonii and indicative of analternative cellulase induction mechanism in this fungu s.The carbohydrate-binding module and linker region that arecharacteristic of some other GH f...
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Báo cáo khoa học: N-terminal deletion of the c subunit affects the stabilization and activity of chloroplast ATP synthase doc

Báo cáo khoa học: N-terminal deletion of the c subunit affects the stabilization and activity of chloroplast ATP synthase doc

... catalysis of isolated CF1.TheN-terminal region of subunit c in CF1is not crucialfor binding of subunit e in vitro. Further studies areneeded to determine candidate residues participating in transmission ... hydrolyticactivity. In vitro binding assays of e with c subunit and immunoblotting analysisEqual amounts of GST or GST–e fusion proteins werebound to glutathione–Sepharose 4B beads in binding buffer[50 ... amounts of thec constructs for 4 h at 4 °C in binding buffer and washedfour times with binding buffer to remove unbound proteins.Subsequently, the beads were suspended in 2· SDS loadingbuffer and...
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Báo cáo khoa học:

Báo cáo khoa học: "Capsular polysaccharide typing of domestic mastitis-causing Staphylococcus aureus strains and its potential exploration of bovine mastitis vaccine developmen" docx

... components which are involved in the pathogenesis in bovine, ovine, and caprine mastitis[29]. In cows, intramammary infection (IMI) due to S.aureus is generally subclinical. Nevertheless, IMI causesconsiderable ... of capsule by the organism and theautoagglutination in the presence of low concentrations of sodium hydroxide. Although the addition of milk whey in some strains was not effective in promoting ... economic losses, particularly milk losses whichaccount for a 10 to 25% of total yield depending on theintensity of inflammation and the stage of lactation [2].Moreover, the presence of S. aureus in...
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