báo cáo khoa học: "Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas)" ppsx

... 9 Discussion Frequency and distribution of sweetpotato EST-SSRs The frequency of SSRs in SSR containing ESTs can accu- rately reflect the density of SSRs in the transcribed region of the genome. Using Sanger and ... in Figure 2). Conclusion In this study, in addition to the characterization of EST- derived SSR markers in cultivated sweetpotato, we designed...

Ngày tải lên: 11/08/2014, 11:21

... sample of trees, in site conditions similar to those of the first sample, included dominant, co- dominant, and intermediate trees, from stands of initial density ranging between 1 ... the growth and development of a tree in a stand ,and Inose (1982), a limited linear one. Our con- text being more similar to that of the former author,...

Ngày tải lên: 08/08/2014, 23:22

... residues of E. carotovora EPG2. Two residues believed to be involved in substrate binding at subsite )2inE. caro- tovora EPG2, namely Arg152 binding the carboxy group and Lys229 interacting with ... few inserted b-strands (1a in Fig. 2), and one large insert of 15 amino acids is present before the first b-sheet of coil 3, which is on the edge of the pronounced sub- strate-bindi...

Ngày tải lên: 20/02/2014, 03:20

... determining the growth kinetics parameters using glucose and arabinose as the sole carbon and energy source. In the presence of glucose and arabinose, the doubling time of the mutant (49.7 ± 0.3 and 52.4 ... Overproduction and purification of recombinant AraL-His 6 . (A) Analysis of the soluble (S) and insoluble (P) protein fraction (20 lg of total protein) of induced...

Ngày tải lên: 14/03/2014, 23:20

... orientation of the inserted fragment were confirmed by restriction enzyme digestion and sequencing of the 5'-junction region by the chain-terminating method. Transfection and selection of the ... post- infection (PI), washed three times with phosphate-buffered saline (PBS), and resuspended in PBS. These cells were lysed by three times of freezing and thawing cycles, an...

Ngày tải lên: 07/08/2014, 17:23

... (fig 23). From the beginning of new terminal and lateral bud initiation, the border between old in 1987, in spite of a very cold winter, only a few (counting from January) ... dates of onset and termination of shoot elongation; iv) seasonal changes of starch accumula- tion in the embryonic shoot; and v) changes in the metabolism of...

Ngày tải lên: 08/08/2014, 19:21

... e domain, i.e., into the N-terminal portion of the CPS domain, resulted in additional decreases in activity, with only 1.21% CFA accumulating upon deletion of 426 aa and 0.84% CFA upon deletion of ... conserved FAD-binding motif in the first 21 aa of the plant oxidase domain. It is hard to envisage how a redox system such as a FAD-containing protein could be involved in the...

Ngày tải lên: 11/08/2014, 11:20

... recorded with an integration time of 20 min. Time lapses were taken at an interval of 30 min for screenings on ZZ and RD2 medium, and 20 min for screenings of in vitro plantlets maintained on REG medium. ... the luc + gene in lines 156, 49 and 111. In addition, T-DNA tandem repeats were identified in lines 156 and 49, and vector backbone sequences were integrated in...

Ngày tải lên: 12/08/2014, 03:20

... events observed in A. hypogaea and in A. ipaënsis and A. duranen- sis: 1) those allowing the amplification in A. hypogaea and A. duranensis and detect a putative locus in the A genome, 2) those allowing ... amplification of a putative locus in A. hypogaea and A. ipaënsis and detect a locus in the B genome, and 3) those allowing the amplification in A. hypogaea, A...

Ngày tải lên: 12/08/2014, 05:20

... cloning and expression of murine L -fucokinase and GDP- L -fucose pyrophosphorylase. Murine L -fucokinase is expressed as two transcripts of 3057 and 3270 base pairs, encoding proteins of 1019 and ... The salvage pathway of GDP- L -fucose in mouse (Eur. J. Biochem. 271)79 Cloning and expression of murine enzymes involved in the salvage pathway of GDP- L -fucose L -fu...

Ngày tải lên: 30/03/2014, 13:20