báo cáo khoa học: " Transcriptomics and molecular evolutionary rate analysis of the bladderwort (Utricularia), a carnivorous plant with a minimal genome" pptx

... 124(1):33-44. doi:10.1186/1471-2229-11-101 Cite this article as: Ibarra-Laclette et al.: Transcriptomics and molecular evolutionary rate analysis of the bladderwort (Utricularia), a carnivorous plant with a minimal genome. BMC Plant Biology ... 11:101 http://www.biomedcentral.com/1471-2229/11/101 Page 15 of 15 Transcriptomics and molecular evolutio...

Ngày tải lên: 11/08/2014, 11:21

... domains influences the membrane interaction and the associated confor- mational changes of the other domain. We compared the pH sensitivities and the membrane interactions of the C and T domains, ... demonstrate that the T domain acts as a chaperone for the C domain. A remarkable feature of this chaperone activity is that it stabilizes at least three different p...

Ngày tải lên: 14/03/2014, 22:20

... percentage of apolar Table 4. Statistical analysis of the differences in the percentage of apolar interface, the hydrophobic contact area of interface, and the percentage of overall interface area. ... for each family of the main dataset. Each apolar interface area was normalized by the total interface area. With the exception of the CS enzyme family, the...

Ngày tải lên: 30/03/2014, 03:20

... homotetramers [4]. Gel filtration analysis of native HYD Js indicated a molecular mass of about 253 kDa, and, as the subunit molecular mass of the His-tagged recombinant HYD Js was estimated to ... pHYD Pf produced a predominant band with an apparent molecular mass of approximately 56 kDa, which is consistent with the calculated mass of the His-tagged transla...

Ngày tải lên: 18/02/2014, 08:20

... 5¢-AAGATGAAACGTG AGACGG-3¢ (CA1); 5¢-GATGACATCTCCATGGAA TAC-3¢ (CA2). The 3¢ region of the hazelnut LOX cDNA was amplified using the following primers: 5¢-GTTT GGAAGAGAGATGCTGG-3¢ (CA3); 5¢-AAAGTTTTT AGATTGAGACACTATTGGGAATT-3¢ ... we carried out a study to characterize hazelnut lipoxygenases at the biochemical and molecular level. Materials and methods Plant material Hazeln...

Ngày tải lên: 21/02/2014, 00:20

... Cag-T4SS, together with potential implications for the current understanding of its mode of action. The cagPAI and the Cag type IV secretion apparatus Gene arrangement and variants of the cagPAI The ... Apart from complete deletions in cag-negative strains, rearrangements include an inversion of cagQ, a duplication of cagA and cagB associated with cagA degenera...

Ngày tải lên: 06/03/2014, 00:21

... standard assay mixtures, the velocity of ABTS oxidation was maximal at 45 °C. The dependence of the rate of ABTS oxidation by lac1 on substrate concentration at pH 3.0 and 45 °C followed Michaelis–Menten ... that product abundance was evaluated in the exponential phase of the reaction (half of the maximum product). To further validate the assays, the optimized cy...

Ngày tải lên: 07/03/2014, 15:20

... somewhat more accessible than the antagonist, as suggested by the solvent-accessible surface area. These data clearly demonstrate the structural importance of the nature o f the amino acid at position ... 0.80 A ˚ for backbone N, C a ,C¢ atoms and all heavy atoms, respectively. The C-terminal part (Ala81–Val85) appears to be much more flexible, with Ala81 far distant from...

Ngày tải lên: 07/03/2014, 16:20

... signal transducer and activator of transcription (JAK ⁄ STAT) [85–87] signaling pathways and the inhi- bition of G s -mediated signaling pathways. A summary of the various intracellular signal ... (NAT) (Pf00797) families PMT-C3 has structural similarity with the catalytic core of TGases (TGase family Pf01841) and arylamine NATs (NAT family Pf00797) [18]. The spatial...

Ngày tải lên: 22/03/2014, 15:21

... the initial and the optimized model was  1.4 A ˚ (calculated on 124 a- carbons). The Ramachandran plot statistics of ChPLA 2 and the complex ChPLA 2 –substrate analog model showed that 97% and ... was used for data processing and data graph- ics generation. The accessible surface of the model was calculated with surface racer, a computer program for fast calcu...

Ngày tải lên: 30/03/2014, 01:20