báo cáo khoa học: " Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis" pot

Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc

... degree of overlap.Careful experimentation to test those hypotheses will need to be performed to determine the binding characteristics of the nucleic acid substrates, both alone and in the presence ... seemsreasonable to make the working hypothesis that the RNA and DNA binding sites are not the same, rather that theyare separate. This does not exclude the possibility that bothnucleic acid binding ... concentration slows the cleavage of the DNA substrate in the absence of RNA (see also Fig. 2D) and that the addition of competitor RNA to an endonuclease reaction masked the 1.025 kb DNA cleavage...

báo cáo khoa học: " In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers" ppt

... decrease of the pH in the peri-apical tissues, and thus there are special demands for the sealing agent s [31]. The major task in reducing the bacterial load, concentrated in the apical region of the root ... have lead to higher treatmentsuccess rates, endodontic treatment and the subsequentrestoration of the tooth should be considered as a ther-apy superior to implantation [3,4]. The choice of a biocompatible ... of the apical area of the root. To achieve this isdesirable to inhibit the growth of the microorganisms i.e. mainly bacteria remaining within the cleaned rootcanal system [41]. On the other...

báo cáo khoa học: " Mass spectrometry analysis of the variants of histone H3 and H4 of soybean and their post-translational modifications" pps

... and H4.2), which varied at the amino acid residueI60 of histone H4.1 and V60 of histone H4.2 (Figure 11). The trypsin digested peptides of histone H4 were directlyapplied to MALDI-TOF/TOF ... copies of H2A,H2B, H3 and H4 [4]. An additional histone, H1 linksthese nucleosomes together along the chromatin chain. Ingeneral, the N terminus of histone H3 and H4, and N and C terminus of H2A ... ty1456.9209ABC6GGKAcGLGK129GLGKAcGGAK161SGRGKGGKGLGKAcGGAK16Identification of the two variants of histone H4Figure 11Identification of the two variants of histone H4. A. MALDI-TOF mass spectrum showing that the amount of the peptide with calculated mass of m/z...

báo cáo khoa học: " Global expression analysis of nucleotide binding site-leucine rich repeat-encoding and related genes in Arabidopsis" pps

... ligands, R proteins may also monitor('guard') the status of the targets of pathogen virulenceeffectors, or the cellular consequences of the actions of these proteins [2,12-16]. The ... weresorted by their gene identifiers, which usually reflects their chromosomal locations, and then the expression pattern of NBS-LRR-encoding and related genes was visually com-pared to their locations ... Thisinconsistency may be due to RACE and MPSS tending to analyze sequences at the ends of transcript or due to the lack of sampling the appropriate tissues or conditions.Together, these data indicate...

Báo cáo khoa học: Structure–function analysis of the ﬁlamentous actin binding domain of the neuronal scaffolding protein spinophilin pot

... NationalInstitute of Neurological Disorders and Stroke to WP. The content is solely the responsibility of the authors and does not necessarily represent the of cial views of the National Institute of Neurological ... description of the molecular components involved in the regulation and maintenance of dendritic spine morphology is funda-mental to our understanding of the functions of the central nervous system. The ... activity of spinophilin. The histo-grams on the right show a quantitative anal-ysis of the polymer length distributions of actin alone (control, top histogram) or in the presence of an equimolar...

Báo cáo khoa học: In silico analysis of the adenylation domains of the freestanding enzymes belonging to the eucaryotic nonribosomal peptide synthetase-like family pot

... manual and automated dockinghave been utilized to map the conserved residuesonto a hypothetical active site structure, to under-stand the role of their conserved residues and predict their ... moiety tethered to the phosphopantetheinylgroup and the free amino group of the downstreamaminoacyl moiety, thus facilitating the translocation of the growing chain onto the next module. The structural ... During the manual docking, steric clashes were removed and the posi-tion of the substrate at the active site was adjusted to estab-lish stabilizing interactions between the atoms of the ligandand...

Báo cáo khoa học: TNFR1-induced activation of the classical NF-jB pathway Harald Wajant1 and Peter Scheurich2 pptx

... stimulation, TRAF2 and the associated cIAPs are recruited to TNFR1-bound TRADD with the help of the C-terminal half of the TRAF domain of TRAF2. Thus, the E3 ligasesTRAF2, cIAP1 and cIAP2 enter the TNFR1 ... K31, together with analy-sis of PKCe knockout MEFs with knockdown of PKCd, revealed further evidence of a crucial role of K63-ubiquitination of the RING domain of TRAF2for recruitment of the ... the leucin zipper motif of IKK1 orIKK2, ubiquitinated RIP1 interacts with the NEMOsubunit of the IKK complex [38,40,42]. The relative contribution of these two interactions to recruitmentof...

Báo cáo khoa học: "ACTH-producing carcinoma of the pituitary with refractory Cushing''''s Disease and hepatic metastases: a case report and review of the literature" pptx

... interests.Authors' contributionsHC performed the bilateral adrenalectomy and togetherwith SP and RS contributed to the conception and design of the manuscript, reanalyzed and interpreted the data and prepared ... prepared the manuscript. SP contributed to the con-ception and design of the manuscript, reanalyzed and interpreted the data and helped in preparation of manu-script. RS. contributed to the conception ... diuretic therapy. The decision to withhold addi-tional ketoconazole treatment was made due to the rapidtumor enlargement noted during the previous trial of the drug. Due to her refractory Cushing's...

Báo cáo khoa học: " Linkage disequilibrium pattern of the ATM gene in breast cancer patients and controls; association of SNPs and haplotypes to radio-sensitivity and post-lumpectomy local recurrence" doc

... illustration of the ATM gene. The distribution of the variations detected in the studied materials along the gene is shown in the upper panel with exonic variants indicated on top of the gene and intronic ... file 2 The estimated halotypes from the case-control analysis of the Norwegian individuals with the number of chromosomes predicted to represent the different haplotypes in: cases, controls and ... this variant and development of costalfractures (P-value 0.055). The impact of this association and other listed in Table 2 have to be interpreted with cau-tion since the number of identified...

Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx

... of the integrity of the exoskeleton.A cDNA of acidic DE25 encoded a 137-amino acid protein and a 22-amino acid signal peptide(AB201776). A partial cDNA of acidic DE29 lacked the 5¢-region and ... rage of isoelectric pointsfrom 3.5 to 10 [25]. Their isoelectric points range from6.5 to 9.2, and the 2D gel shows no protein spots in the acidic range from 3.5 to 5.5, corresponding to the members ... (12%) and Gly (11–12%), and proteins in the acidic S group, whilesimilar otherwise to those in the neutral group, wereadditionally characterized by an abundance of Ser (9–12%). The members of acidic...

Xem thêm