báo cáo khoa học: "Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin" potx

... Access Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin Nuria Sánchez-Pons, Sami Irar, Nora García-Muniz and Carlos M Vicient * Abstract Background: Camptothecin is ... 11:91 http://www.biomedcentral.com/1471-2229/11/91 Page 13 of 20 Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin Sánchez-Pons e...

Ngày tải lên: 11/08/2014, 11:20

... studies of the structure and binding sites of enzymes. 8. Inhibitor binding to ribonucleases. J Mol Biol 45, 491–511. 33 Ui N (1971) Isoelectric points and conformation of proteins. I. Effect of urea ... new class of surrogates for the phosphoryl group. Results and Discussion Sulfonimides as inhibitors of RNase A We began by determining the ability of three backbone anal...

Ngày tải lên: 14/02/2014, 22:20

... investigated the chromatographic behav- iour of recombinant wild-type enzyme in the presence of ATP and Mg 2+ as an activator and P i as an inhibitor. Figure 5 shows that the enzyme exists and functions ... identified two putative effector sites, and suggested that effector site 1, close to interface A, might interact with BPG and Ap 4 A, while ATP and ADP might bind to eff...

Ngày tải lên: 15/02/2014, 01:20

... Asp142, 2¢ and 3¢OH of the ribose and three water molecules with coordination distances of approximately 2.4 A ˚ . The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino ... a disease with symptoms of self mutilation and mental retardation [4]. The underlying mechanisms of Lesch– Nyhan syndrome are still not well understood, and the abs...

Ngày tải lên: 15/02/2014, 01:20

... inhibitor is known for hydantoinases, therefore the productive transition states of hydantoin, d-p-HPH, l-p-HPH and DHU were docked into the active site of HYD Js [28] to simulate the mode of sub- strate ... [27]. The three SGLs of HYD Js , i.e. SGL1, SGL2 and SGL3, correspond to residues 60–71, 91–99 and 151–161, respectively. On the basis of the homol- ogy model, the SGL...

Ngày tải lên: 18/02/2014, 08:20

... k 2 values to change. A B Fig. 6. Model and simulations of cytochrome c reduction by NOS enzymes. (A) Scheme of cytochrome c reduction. The model uses four kinetic rates: dissociation (k 1 ) and association ... association (k 2 ) of the FMN and FNR subdomains; FMNH • reduction rate (k 3 ) and cytochrome c reduction rate (k 4 ). For simplicity, k 1 and k 2 are assumed to be...

Ngày tải lên: 18/02/2014, 11:20

... Comparison of rates and kinetic isotope effects using PEG-modified variants and glycoforms of glucose oxidase: the relationship of modification of the protein envelope to C–H activation and tunneling. ... Kostic NM (1993) Gating of photoinduced electron-transfer from zinc cytochrome-c and tin cyto- chrome-c to plastocyanin – effects of solution viscosity on rearrangement...

Ngày tải lên: 18/02/2014, 11:20

... to a site where it can best be neutralized by protonation – the N5 position. Electron transfer from PSI to flavodoxin Fd and Fld differ in size and in the chemical nature of their redox cofactors. ... orientation of the N5 of the flavin, the hydride to be transferred, and C4 of the nicotinamide. In FNR, displacement of the C-terminal Tyr appears to be required for the in...

Ngày tải lên: 18/02/2014, 11:20

... N3 to the backbone O of Phe133, and from O4 to the backbone N and the side chain of Thr131, with the ribose moiety anchored by two hydrogen bonds, one from 2¢-OH to the side chain of Asp70, and ... the backbone N of Gly63, and the phosphate group forming hydrogen bonds from O1 to Arg55, from O2 to the backbone N and the side chain of Thr138, and from O3 to th...

Ngày tải lên: 18/02/2014, 16:20

... aniso- tropy of the free minifiber, A B is the anisotropy of the mini- fiber bound to the penton base, [Lig B ] is the concentration of the bound ligand (minifiber), and [Lig tot ] is the total con- centration ... frac- tions of interest were then concentrated and stored at ) 80 °C. TMR labeling and binding assays A five-fold excess of malemide-derivatized TMR was added to a s...

Ngày tải lên: 19/02/2014, 06:20