... were used to determine background staining. Afterwashing of unbound antibody, samples were analysed on a FACScan flow cytometer (Becton, Dickinson and Company,Franklin Lakes, NJ, USA), and the ... staining of PMA (phorbol 12-myristate 13-acetate)/ionomycin activated T cells. (a) We detected interferon (IFN)-γ staining but no interleukin (IL)-4 staining. Mul-tiplex cytokine assay was ... chains and one α-2(I) chain, with each α chain containing 1,014 amino acid res-idues. In mammals, CI is highly conserved, and there is a 92%homology at the amino acid level between human and...