Báo cáo y học: "Age-related waning of in vitro Interferon-γ levels against r32kDaBCG in BCG vaccinated children" ppt

... stimulation indices in vaccinated children decreased in the older children concurring with an increase in the incidence of TB. Conclusion: Significantly high levels of in vitro IFN-γ demonstrated in BCG ... results of our earlier study [7,8], based on in vitro T cell assays using tuberculin as a stimulant, revealed that the effect of BCG wanes with age. Waning...

Ngày tải lên: 11/08/2014, 10:23

... immunoglobulin chains were also found to be significantly increased in aged thymocytes. Understanding the changes in gene expression in thymocytes with age may hold the key in determining thymocyte ... 3. Protein levels of immunoglobulin increase within the thymocytes with age. Western blot analysis of IgM heavy chain protein levels with successive age. This image is repre...

Ngày tải lên: 03/11/2012, 11:35

... recombinant protein containing the C-myc epitope followed by six histidines at the C-terminus. A plasmid containing the wild type version of CCR5 was constructed by introducing the coding sequence ... The resulting XhoI/ApaI fragment was cloned into the corres- ponding sites in the polylinker region of the pcDNA3.1- myc-His expression vector (Invitrogen, Groningen, the Netherlands) in...

Ngày tải lên: 08/03/2014, 09:20

... access [14]. In contrast to all Fe-hydrogenases including HydA of S. obliquus,the enzyme of C. reinhardtii has an interesting additional protein domain. A small insertion of 45 amino acids between ... synthesis of s ome enzymes involved in glycolysis or sugar-phosphate metabolism [54]. HydA belongs to a new class of Fe-hydrogenases HydA of C. reinhardtii, the first isolated gen...

Ngày tải lên: 08/03/2014, 22:20

... expression of a variety of single mitochondrial-type AACs in E. coli. After IPTG-induction we were able to obtain functional integration of the various mitochondrial-type AACs into the cytoplasmic ... trans- ferredtonewreactionvesselsandheatedto95°Cfor 10 min to prevent any further reaction. An aliquot of the exported radioactive solution was used for separation by thin-layer chroma...

Ngày tải lên: 18/03/2014, 01:20

... of cofactor for TDP enzymatic activity was examined by determining the specific activity of TDP by following the cleavage of 1 m M of substrate by 0.125 l M of enzyme in reaction buffer containing ... produced no increase in A 415 , confirming that the hydrolysis of the tyrosine-DNA phosphodiester bond detected before was indeed produced by the activity of purified recombinant...

Ngày tải lên: 31/03/2014, 21:21

... a 1 Gly56 2.6H Tyr50 a 2 Gly56 3.5H Tyr50 a 2 Gly56 2.6H Tyr50 a Ala59 3.5H Tyr50 a Ala59 2.6H Tyr50 b Ala59 3.5H Tyr50 b Ala59 b 1 Tyr50 a 1 Gly56 b 2 Tyr50 a 1 Gly56 b 1 Tyr50 a 2 Gly56 b 2 Tyr50 ... during the early stages of HIV-1 replication, binding of NCp7 to the C-terminal domain (80–96)Vpr [8] may expose the N-terminal (1–39) portion of the protein and allow its tight binding t...

Ngày tải lên: 31/03/2014, 23:20

... rather homogeneous in terms of baseline depression severity and duration of treatment; these analyses evaluated treat- ment effects with a variety of antidepressants, including tricyclic antidepressants, ... picture of response to antidepressant therapy with venlafaxine. Correlation between definitions of response (final on ther-apy)Figure 4 Correlation between definitions of re...

Ngày tải lên: 08/08/2014, 23:21

... small interfering RNA against PHD-1 or PHD-2 or the pan-hydroxylase inhibitor dimethyloxalylglycine (a 2-OG analogue, and an inhibitor of both PHD and FIH) resulted in NF-κB activation via serine ... occurs at the β-carbon of the asparagine residue, consequently (by way of steric hindrance) preventing the interaction of the HIF-α C-TAD with the cysteine/histidine-rich 1 domain of...

Ngày tải lên: 09/08/2014, 01:22

... 1). Cytokine mRNA expression in zymosan A injected joints Expression of mRNAs encoding various cytokines was investigated in knee joints of ob/ob and +/? animals on days 1 and 14 after zymosan A injection. ... shown). Circulating levels of SAA increased in all animals during the first 3 days after intra-articular zymosan A injection (Table 1). Early after zymosan A injection the...

Ngày tải lên: 09/08/2014, 01:23