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Báo cáo y học: "Phenotype and in vitro function of mature MDDC generated from cryopreserved PBMC of cancer patients are equivalent to those from healthy donors" ppt

Báo cáo y học:

Báo cáo y học: "Phenotype and in vitro function of mature MDDC generated from cryopreserved PBMC of cancer patients are equivalent to those from healthy donors" ppt

... healthy donors (Fresh Healthy) , cryopreserved PBMC of healthy donors (Cryo. Healthy) , and cryopreserved PBMC of cancer patients (Cryo. Cancer) . A. Sur-face phenotype: Immature and mature MDDC from ... (Fresh Healthy) , cryopreserved PBMC of healthy donors (Cryo. Healthy) , and cryopreserved PBMC of cancer patients (Cryo. Cancer) Figure 2Effect of maturation on MDDC derived from fresh PBMC of healthy ... studied by comparing the phenotypic and functionalproperties of mature MDDC derived from cryopreserved PBMC of healthy donors to those from fresh PBMC of healthy donors. Because PBMC from cancer patients...
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Báo cáo y học: "Glucosamine prevents in vitro collagen degradation in chondrocytes by inhibiting advanced lipoxidation reactions and protein oxidation" pdf

... inhibit cytokine (interleukin-1[IL-1])-induced activation of chondrocytes and nuclear factor-kappa-B activity and to upregulate type II IL-l decoy receptor[18,19]. In vivo, glucosamine helps ... of effects in in vitro chondrocyte and explant cul-tures [9-13]. These effects include stimulation of proteoglycansynthesis, inhibition of the degradation of proteoglycans, and inhibition of ... glu-cosamine inhibited Cu-induced carbonyl modification of lipo-proteins, indicating that glucosamine also traps reactivecarbonyl compounds. In addition to aminoguanidine and pyri-doxamine, therapeutic...
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Báo cáo y học: "Prion propagation in vitro: are we there yet"

... directly to wild type mice. To obtain infectivity in wild type mice, synthetic prions were Int. J. Med. Sci. 2008, 5 352serially passaged to wild type mice only after primary transmission into ... novel in vitro system re-ferred to as the autocatalytic conversion assay. The principle of this assay heavily relies on selective re-folding of denatured rPrP in the absence of PrPSc. In essence, ... characteristic of the amyloid fibrils generated by this assay. Prusiner and colleagues in- duced amyloid fibrils from recombinant mouse PrP 89-230 and used these synthetic prions to infect trans-genic...
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Báo cáo y học: " An efficient in vitro-inoculation method for Tomato yellow leaf curl virus" ppsx

... wereconsidered healthy with no obvious TYLCD symptoms and they continued to grow normally (Table 1). Theresults obtained showed the feasibility to inoculate effi-ciently in vitro tomato plants with TYLCV ... TYLCV and typicalsymptoms of TYLCD with leaf curling and yellowingwere observed.For symptom development, NS16 tomato microshootsinoculated with the empty plasmid did not show anyTYLCD symptoms ... possibility to identify the mechanism of resistance against TYLCV in wild tomato genotypesusing in vitro cultures.Discussion In this study, an efficient in vitro- inoculation method forTYLCV was...
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Báo cáo y học: " Physical and in silico approaches identify DNA-PK in a Tax DNA-damage response interactome" pot

... directly influenced by the amount of measurable protein and indirectly influenced bystrength of binding. Thus, we combined the data, in which the Tax interactome was analyzed as describedabove, from ... especially true given the exponentialincrease in technical ability to identify protein-proteininteractions, compounded by the inherent increases in false-positives (protein-protein interactions of ... working stock of Jurkat nuclearlysate (Active Motif, Carlsbad CA) was prepared by dilut-ing 25 μg lysate to a total volume of 75 μL in incubationbuffer. The lysate was pre-cleared by adding...
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Báo cáo y học: " Diagnosing asthma in general practice with portable exhaled nitric oxide measurement – results of a prospective diagnostic study" ppsx

... lung function laboratory of the University MedicalHospital before investigation with bodyplethysmography and bronchial provocation, as forced inspiratory and expiratory manoeuvres could lead to ... were instructed not to use any bron-chodilator or inhaled steroid and stop smoking and drinking coffee twelve hours before visiting the lung func-tion laboratory. Patients with previously established ... also found in other inflammatory disorders includingsinus disease [10] and viral upper respiratory tract infec-tion [11], but not in patients suffering from chronicobstructive pulmonary disease...
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Báo cáo y học: " ''''Diagnosing Asthma in General Practice with Portable Exhaled Nitric Oxide Measurement – Results of a " pps

... Heidelberg, Germany, 2Department of Primary Care Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands and 3Department of Cardiology, Pulmonology and Angiology, Medical ... improves diagnosticefficiency compared to the £ 12 ppb cut-off point.We would like to correct the following points in the man-uscript: In the Results section of the Abstract lines 6–7 shouldread ... "three". In line 4 "12 ppb" should say "16 ppb". The sentencestarting in line 5 and ending in line 6 should read: "Sensi-tivity was 78% (95%CI 63–89), specificity was...
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Báo cáo y học: "Accelerated decline in lung function in smoking women with airway obstruction: SAPALDIA 2 cohort study" ppsx

... differ-ence in lung function decline per pack of cigarettesmoked.Reduced flow rates are due to a combination of airwaynarrowing and decreased lung recoil [9]. Predisposition to airway narrowing and ... studiescomparing effects from smoking on lung function between men and women may be due to difficulties in separating out effects from recent smoking and past smok-ing. In both men and women recent smoking ... smoking history were measured in 1991 and 2002. The aim of the present analysis is to assessthe long term effects from smoking on change in lung function and to compare effects between men and women....
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Báo cáo y học: " Processing sites in the human immunodeficiency virus type 1 (HIV-1) Gag-Pro-Pol precursor are cleaved by the viral protease at different rates" ppt

... further suggest that early dimerization of thePR and RT domains may serve as a regulatory element to influence the kinetics of processing withinthe Pol domain.FindingsThe retroviral protease ... in sequence to the viral Gag-Pro-Pol polyprotein precursor [23,27] (Fig. 1A). Intrinsicprotease activity was inactivated by a D25A substitution of the catalytic aspartate of the PR domain to ... translationally silent substitutions were inserted in the area frameshift to reduce secondary structure and translational pausing during expression. The activity of the intrinsic protease was inactivated...
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Báo cáo y học: "yclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ – prostaglandin E2 dependent proliferation of growth plate chondrocytes" ppsx

... Klein T, Backman JT, Saal JG, Nüsing RM, Fritz P: Expres-sion of cyclooxygenase 1 and cyclooxygenase 2 in human syn-ovial tissue: Differential elevation of cyclooxygenase 2 in inflammatory joint ... plate in situ and in vitro. PGE2 synthesis was determined by mass spectrometry, cellproliferation by DNA [3H]-thymidine incorporation, mRNAexpression of cyclooxygenases and EP receptors by ... was determined by size and dideoxysequencing.ImmunohistochemistryFor immunohistochemistry, the epiphyseal plate with neigh-bouring bony metaphysis and epiphysis including the kneejoint were...
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