Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx

... al.: Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B. Journal of Immune Based Therapies and Vaccines 2010 8:9. Submit your ... 9 ORIGINAL RESEARCH Open Access Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal...

Ngày tải lên: 11/08/2014, 08:21

... Tyr183 in catalysis In previous crystallographic studies it was shown that the hydroxyl side chain of Tyr183 is within hydrogen bonding distance of the N-acetyl carbonyl oxygen of the sugar residue ... (1995) Stereochemistry of chitin hydrolysis by a plant chitinase/lysozyme and X-ray structure of a complex with allosamidin: evidence for substrate assisted catalysis. Biochemistr...

Ngày tải lên: 22/02/2014, 04:20

... to homogeneity by HPLC and digested with t rypsin. The tryptic peptides were separated by HPLC and some of them were totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass spectrometry ... 2Cys-Prx enzymes previously described i n yeast, mammals and plants, Ch-Prx1 d isplayed antioxidant and peroxidase activities. The enzyme could reduce hydro- gen peroxide as...

Ngày tải lên: 08/03/2014, 16:20

... doi:10.1046/j.1432-1033.2002.03335.x by the addition of proFIX18 and proPT18, indicating that the Conus carboxylase, like the bovine carboxylase, is activated by the human propeptides. To compare the potency of the effect of these ... carboxylase activity. The effect of proFIX18, proPT18 and pro-e-TxIX/12 on FLEEL carboxylation by the recombinant bovine carboxylase (closed symb...

Ngày tải lên: 17/03/2014, 10:20

... potentially lethal effects by specifically and potently blocking a variety of ion channels, including those that conduct Na + ,K + ,andCa 2+ . Therefore, neurotoxins have been employed as useful ... microliters of 4-vinylpyridine were then added, and alkylation was allowed to proceed for 3 h at room temperature. The S-pyridylethylated proteins were separated from the reagents by C18...

Ngày tải lên: 18/03/2014, 01:20

... TR-like protein (54% identity and 73% similarity) from Sulfolobus solfataricus and shows a relat- ively high homology to the TR of E. coli (34% identity and 54% similarity). This protein possesses ... However, it can be reduced directly by ApTR, indicating that it has the broader substrate specificity than that of low M r TRs. Typically, enzymes of this family contain two ide...

Ngày tải lên: 23/03/2014, 21:20

... Naim 2 and Tosso Leeb 1 1 Institute of Animal Breeding and Genetics and 2 Institute of Physiological Chemistry, School of Veterinary Medicine Hannover; 3 Institute of Molecular Biology, Medical ... genomics, human, mouse, dog. The initial completion of the human genome sequence revealed that the function of only about 15 000 of the estimated 35 000 genes in the human...

Ngày tải lên: 23/03/2014, 21:20

... kDa in polyacrylamide gels. Antibody staining of sections from bovine prelactating and lactating mammary gland using monoclonal and polyclonal antibodies has shown that PAS III is largely concentrated ... the algorithm of Nielsen et al. [28], the extracellular region of bovine and human MUC15 offer 22 and 14 O-glycosylation sites, respectively. The majority of these po...

Ngày tải lên: 24/03/2014, 04:21

... design and revision of the study and performed the statistical analysis. RP participated in the design and revision of the study. AS partic- ipated in the analysis and interpretation of data and ... immunoreactivity of IgM, IgG and IgA specific to Sip1 C-ter varied significantly among various groups of patients analysed. The precise significance of the isotypes of an...

Ngày tải lên: 09/08/2014, 08:22

... declare that they have no competing interests. Authors’ contributions HSP, SYP, KML and CHL conceived and designed the experiments. JIK, GSJ, YYK, GYJ, HSK, WDW and CHL performed experiments and ... polymorphism analysis of Varicella-Zoster Virus isolated in Korea. J Kor Soc Virol 1991, 21(2):201–210. 8. Sohn YM, Park CY, Hwang KK, Woo GJ, Park SY: Safety and immunogenic...

Ngày tải lên: 11/08/2014, 21:22