Báo cáo y học: "Antiviral activity of Engystol® and Gripp-Heel®: an in-vitro assessment" pptx
Báo cáo y học: "Antiviral activity of Engystol® and Gripp-Heel®: an in-vitro assessment" pptx
... of MedicalVirology and Epidemiology of Virus Diseases of theHygiene Institute at University of Tübingen, respectively.For IFN assaysHeLa cells (University of Jena, Germany) were incubatedwith ... seems likely that Engystol® and Gripp-Heel® also modulate host defencemechanisms.Type 1 IFN production plays an important role inantiviral response and involves a large family of multi-functional ... in type 1 IFN production. Engystol® and Gripp-Heel® are used prophylactically toward off viral infections and are valued for their goodtolerability and lack of any known adverse effects [2,5].The...
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Báo cáo y học: "Antiviral activity of a-helical stapled peptides designed from the HIV-1 capsid dimerization domain" pps
... requirement, we synthesized NYAD-201,NYAD-202 and NYAD-203, a soluble analog of NYAD-201. We also synthesized the linear analog of NYAD-201 (NYAD-209) and a mutant analog of NYAD-201(NYAD-233) after ... assembly being reversible byVSV-G pseudotyping [75].Anti-HIV-1 activity and cytotoxicity of stapled peptides incell-based assaysWe measured the anti-HIV-1 activity of NYAD-201 and its analogs ... microscopy, isothermal titration calorimetry and sedimentation equilibrium analyses and analyzed data; FC carried out theantiviral assays and analyzed data; XZ helped in preparing and purifyingproteins;...
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Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"
... Sammynaiken3 and Jian Yang1, 1. Drug Design and Discovery Research Group, College of Pharmacy and Nutrition, University of Saskatchewan, 110 Science Place, Saskatoon, SK S7N 5C9, Canada ... types of cancer, and evaluated the anticancer activity of the PR domain against human hepatoma HuH7 cells through both direct administration of recombi-nant His6-tagged PR and cDNA transfection. ... G2/M phase of the cell cycle and induces apoptosis [10-12]. An imbalance in the amount of RIZ1 and RIZ2 may be an important cause of cancer develop-ment [1]. In fact, silencing of RIZ1 expression,...
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Báo cáo y học: "Antiviral therapy of HCV in the cirrhotic and transplant candidate"
... importance of waiting time, those awaiting liver transplant are more ill. The resulting poor synthetic function, presence of hepatic encephalopathy and hypersplenism limit the candidacy of patients ... Transplantation Society Expert Panel. Report of the first International Liver Transplantation Society expert panel consensus conference on liver transplantation and hepatitis C. Liver Transpl ... study of the tolerability and efficacy of antiviral therapy in hepatitis C virus-infected patients awaiting liver transplantation. Liver Transpl 2002;8:350-5. 16. Thomas RM, Brems JJ, Guzman-Hartman...
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Báo cáo Y học: Transcriptional activity of interferon regulatory factor (IRF)-3 depends on multiple protein–protein interactions pdf
... lg), and harvested 2 days aftertransfection. Chloramphenicol acetyl transferase (CAT) activity and b-galactosidase activity were measured inextracts of transfected cells [21], and CAT activity ... intrinsictranscriptional activity. Fig. 2. The transcriptional activity of IRF-3 depends on synergy betweentwo sets of residues and is entirely dependent on mammalian CBP. (A)Transcriptional activity ... contact withboth the N- and C-termini of CBP, and on the physicalintegrity of CBP.We also tested the ability of IRF-3E7 and human p300 ormutant derivatives [27] to activate transcription from theISRE·3CAT...
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Báo cáo Y học: Amidolytic activity of prostatic acid phosphatase on human semenogelins and semenogelin-derived synthetic substrates docx
... Luiz Juliano2, MicheÁle Ferrer1, Thierry Moreau1 and Francis Gauthier11Laboratory of Enzymology and Protein Chemistry, INSERM EMI-U 00-10, University FrancË ois Rabelais, Faculty of Medicine,Tours, ... polyclonalantibody against PSA does not reveal any band afterimmunoblotting; (c) the amidolytic activity is strictlyassociated with the peak of PAP after reversed-phaseHPLC, and no trace of ... Fractions with enzymatic activity wereanalysed by MALDI TOF MS and reversed-phase HPLCon a C4 cartridge. A molecular mass of 47 798 Da wasdetermined by MS and a single, symetrical peak was...
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Báo cáo Y học: Bromoperoxidase activity of vanadate-substituted acid phosphatases from Shigella flexneri and Salmonella enterica ser. typhimurium doc
... recombinant PhoN-Sf and PhoN-Sesubstituted with vanadate also catalyzed the oxidation of bromide and the enantioselective oxidation of thioanisole[19,20].MATERIALS AND METHODSMaterialsAll standard ... Dumay1, Qianning Liao2, Alex J. Lange2 and Ron Wever11Institute for Molecular Chemistry, University of Amsterdam, The Netherlands;2Department of Biochemistry, Molecular Biology and ... active siteplay a very important role in tuning the activity and specificity of these enzymes. Identification of these residueseven with a full knowledge of the crystal structure and sequence is...
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Báo cáo y học: " Antiretroviral activity of the aminothiol WR1065 against Human Immunodeficiency virus (HIV-1) in vitro and Simian Immunodeficiency virus (SIV) ex vivo" pptx
... OAO,JB, and AWH grew and treated the cells and performed thecytotoxicity assays and immunoassays for virus titer. OAOprovided important conceptual input regarding the cyto-toxicity assays. GF ... on days 3, 7, 10, 14 and 17.ResultsAnti-HIV-1 activity and cytotoxicity of WR1065 in human TCBsIn HIV-1-infected human TCBs, the HIV-1 titers, deter-mined in the absence of drug, ranged ... toxicityat the doses chosen.Anti-HIV-1 activity of AZT, with and without WR1065, in human TCBsFigure 1B shows inhibition of HIV-1-replication, and cellsurvival determined by Trypan blue,...
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Báo cáo y học: " Differential activity of candidate microbicides against early steps of HIV-1 infection upon complement virus opsonizatio" pdf
... CADA (0.2 and 2 μg/ml), T20 (0.5 and 5 μg/ml),IgG2G12 (1 and 10 μg/ml), IgG2F5 (7.5 and 25 μg/ml),IgG12G5 (2.5 and 12.5 μg/ml), and IgG1B12 (1 and 10 μg/ml) and polyclonal anti-gp160 antibodies ... experiments. MAJ, HS and LB analyzed data and wrote the paper. JB and DS participated in the design of the study and provided HHA and GNA. TWB provided CADA and helped draft the manu-script. GV participated ... design and coordination of the study and helpeddraft the manuscript. LB conceived the study, participated in its design and coordination, analyzed data and wrote the paper.All authors read and...
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Báo cáo y học: "Antioxidant activity of tuberosin isolated from Pueraria tuberose Linn" pot
... [Puetuberosano l], (3’ -hydroxy-4’-phenoxy-a,b-epoxychalcan-a’ol)] [9], Ptero-carpanoids [Hydroxytuberosin, Anhydroxytuberosin(3-O-meth ylanhydrotuberosin)] [10], and Tuberosin [11].The powder of ... anti-fertility [12]. One of its* Correspondence: yaminiok@yahoo.comDepartment of Medicinal Chemistry, Institute of Medical Science, BanarasHindu University, Varanasi-221005, IndiaPandey and ... Pandey and Tripathi: Antioxidant activity of tuberosinisolated from Pueraria tuberose Linn. Journal of Inflammation 2010 7:47.Submit your next manuscript to BioMed Central and take full advantage...
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