... contamination during sample prep-aration for post-PCR analysis. Real-time RT-PCR assayshave been widely utilized for early diagnosis of manyother animal viral diseases [7,8]. In this study a ... completesamplification and analysis in a closed system, has manyadvantages over conventional RT-PCR methods: lowerchance of contamination, allows quantitative measure-ment of RNA, more rapid to ... Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu730046, ChinaFull list of author information is available at the end of the articleTian...