Báo cáo khoa hoc:" Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule" pptx

... purposes) Journal of Negative Results in BioMedicine Open Access Brief report Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native ... native granulysin. Thus, the addition of GFP to the C-terminus of granulysin obscures the signal(s) that cytotoxic l...

Ngày tải lên: 11/08/2014, 08:20

... release of pro-inflammatory cytokines and oxidizing species production after septic shock. HSP70 is the most important protein in HSP family to generate a protective effect against injuries in the ... However, the volume of infusion in every administration was strictly controlled according to body weight to be the same in the three groups, and the MAP still dec...

Ngày tải lên: 13/08/2014, 03:20

... tree, in tree’ wood, could be called the ’prehistory’ of the wood, as opposed to the history of ’outside-tree’ wood. The ’history’ of the material includes cutting, drying and ... from the point of view of the mechanical standing of trees as well as that of the loading history applied to the material before tree felling. St...

Ngày tải lên: 08/08/2014, 19:21

... d 1 heme-binding domain of cytochrome cd 1 . Therefore, we tested for the binding of d 1 heme to purified NirF. The addition of d 1 heme to the NirF resulted in the appearance of a distinctive visible ... residue that in NirS is the proximal axial ligand to the d 1 heme. Replacement of an equivalent His, His41, in NirF by Ala abolished binding of the heme...

Ngày tải lên: 15/02/2014, 01:20

... conformation, the SH2 domain binds to pY i on the C-terminal tail, and the Src homology 3 (SH3) domain binds to the linker between the SH2 and kinase domains at the back of the small lobe, preventing the ... whether our findings can be applicable to other protein kinase families. Interest- ingly, the tetrameric subunit structure of the Abl/Arg and Tec kinase fam...

Ngày tải lên: 18/02/2014, 11:20

... 2). These data indicate that both peptides accumulate in inclusion bodies, and that Ab(L1–40) is the dominant protein in the inclusion bodies. In contrast, the major protein in the Ab(M1–42) inclusions ... fused to a larger protein to prevent degradation. A common drawback of such approaches is the cost of the affinity resins used to isolate the fusion...

Ngày tải lên: 18/02/2014, 13:20

... from the entire cod- ing sequence of the MCPIP gene. The coding sequence lacking the PIN domain was obtained in two steps. First, the fragment of 423 bp situated in front of the PIN domain was ... proteins, 73% are proteins involved in mRNA stability, but there are also proteins involved in other cellular processes, such as actin. Further stud- ies characterizing pro...

Ngày tải lên: 18/02/2014, 14:20

... transcriptional initi- ation sites. Determining the translation initiation site of isoform 2 KLK11 Messenger RNA for isoform 2 contains two initiation codons near the 5¢ end. To examine whether the first initiation ... pro- moter use of the KLK11 gene appears to link to the splicing pathway of KLK11 mRNAs and to regulate the trafficking of KLK11 isoforms in the t...

Ngày tải lên: 19/02/2014, 06:20

... used, the 4¢-phosphopantothenoyl- CMP intermediate was converted to PPC (in presence of cysteine) or to PP (in the presence of cysteamine) or to 4¢-phosphopantothenoylcysteine methyl ester (in the Fig. ... synthesized in five steps from D -pantothenate. The Dfp flavoprotein catalyzes the synthe- sis of the coenzyme A precursor 4¢-phosphopantetheine from 4¢-phosphopa...

Ngày tải lên: 19/02/2014, 12:20

... lumen [43,44]. Considering the importance of the intestinal barrier in the process of oral prion infection, this find- ing might help clarify the entry and routing of PrP Sc in the early steps of infection. ... and intestinal epithelium (ie) (M, insert) of the posterior intestine (pi). In the CNS, the labeled divisions are the telencephalon (t), mesencephalon...

Ngày tải lên: 19/02/2014, 16:20