Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx

... BRL, Grand Island, NY), 0.25 µg of each primer, 6 mM MgC12 in each CD4 reaction and 4 mM MgC12 for CD8 reac- tions, 2 mM total dNTP's, and 0.5 U of Taq Polymerase. For CD4 and CD8 reaction ... RNA extraction and reverse transcription is determined by quantification of GAPDH mRNA by QC-RT-PCR. Each value for quantity of CD4 or CD8 mRNA was st...

Ngày tải lên: 11/08/2014, 08:20

... mesotrypsin can activate a given PAR isoform. Furthermore, tissue- and species-specific glycosylation of PAR can also alter activation properties, which may account for some of the conflicting data published. The ... k 1 and k )1 denote the forward and reverse rate constants of the formation of the noncovalent complex EI; k 2 is the rate constant of the formation of th...

Ngày tải lên: 30/03/2014, 10:20

... trypanosomiasis, is caused by the hemoflagellate Trypanosoma cruzi and is an important cause of heart disease in South and Central America. Patients with Chagas' disease have an early impairment of baroreflex ... manifestations of the disease. Chagas' disease, or South American trypanosomiasis, is caused by the hemoflagellate Trypanosoma cruzi and has different for...

Ngày tải lên: 11/08/2014, 08:20

... actin-7 as a reference, we validated that the differential responses for GSTF6 and PR-1 are consis- tent with the microarray dataset (data not shown). Bioinformatic Analyses Data analysis was performed ... 141:436-445. 26. Iida K, Seki M, Sakurai T, Satou M, Akiyama K, Toyoda T, Konagaya A, Shinozaki K: RARTF: Database and tools for complete sets of Arabidopsis transcription...

Ngày tải lên: 12/08/2014, 03:21

... and aborted fetuses of pigs. Materials and Methods Viruses and sample preparation The JEV isolate KV1899, Anyang 300 (attenuated vaccine strain) and Nakayama strain were used as standard virus for ... Burkhalter KL, Kulasekera VL, Lambert AJ, Lanciotti RS, Hunt AR, Ryan JR. Comparison of vero cell plaque assay, TaqMan reverse transcriptase polymerase chain reaction RNA...

Ngày tải lên: 07/08/2014, 18:20

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Ngày tải lên: 07/08/2014, 18:21

... regression shows that the method can be used as an excellent approximation of FI traces and to quantitatively esti- mate the contribution of each phase. The average val- ues of amplitudes and half-times (t 1 ... thylakoid membranes. Such analysis is needed for a a more thor- ough use of FI in the study of PSII electron transport and to obtain a more complete analysis...

Ngày tải lên: 19/02/2014, 05:20

... M., Nitta, K., Kawauchi, H., Takayanagi, Y. & Oyama, F. (1991) Comparative base specificity, stability, and lectin activity of two lectins from eggs of Rana catesbeiana and R. japonica and liver ribonuclease ... S., Barone,G.&DiDonato ,A. (2001)Contributionofchaintermini to the conformational stability and biological activity of onconase. Biochemistry 40, 9097–9103. 9. Ok...

Ngày tải lên: 19/02/2014, 12:20

... intermediate not entering the cell as an explanation. There is a second alternative explanation for the apparent absence of channeling of Glu6P. Glu6P just made in the pathway could be strongly channeled ... signa- ture of that channeling would be lost because Fru6P was apparently not channeled. The data in Table 3 show the method for and the results of calculating t...

Ngày tải lên: 19/02/2014, 18:20

... insulin-mediated DNA synthesis. J Cell Sci 113, 4499–4510. 41 Honda A, Nogami M, Yokozeki T, Yamazaki M, Nakamura H, Watanabe H, Kawamoto K, Nakayama K, Morris AJ, Frohman MA et al. (1999) Phosphatidyl- inositol ... positive feedback model (Fig. 1A) as an example, we have shown that the relative amplifica- tion approach allows quantitative analysis of local and global sensitivities, e...

Ngày tải lên: 07/03/2014, 21:20