Báo cáo khoa hoc:" A heparin binding synthetic peptide from human HIP / RPL29 fails to specifically differentiate between anticoagulantly active and inactive species of heparin" pptx

... Access Research A heparin binding synthetic peptide from human HIP / RPL29 fails to specifically differentiate between anticoagulantly active and inactive species of heparin David E Hoke 1,2 , Daniel D Carson 3 ... the coagulation cascade by inactivating serine proteases, such as factor Xa (FXa) and thrombin. The interaction between ATIII an...

Ngày tải lên: 11/08/2014, 08:20

... using the Pfu DNA polymerase, the forward primer S64XHOF 5¢-AAGAAAC TCGAGGTCGAAGA-3¢ (coordinates 103–121, XhoI site underlined) and the reverse primer SEF97R 5¢-ATACATTCCCC GAATTCAGCCA CCTCC-3¢ (positions ... thio-b- D -galactoside (IPTG). The induced protein was affinity-purified using Ni-chelating Sepharose resin (Amersham Pharmacia Biotech.) and used as an antigen to raise polyclonal...

Ngày tải lên: 23/03/2014, 21:21

... 200 wt 1A 2A 3A 4A 5A N10 M10 C10 LVCEETANYLVIWCIDLEVE AAAAETANYLVIWCIDLEVE LVCEAAAAYLVIWCIDLEVE LVCEETANAAAAWCIDLEVE LVCEETANYLVIAAAALEVE LVCEETANYLVIWCIDAAAA LVCEETANYL TANYLVIWCI VIWCIDLEVE Fig. 5. Five residues of Cdc37 are essential for the binding ... A client -binding site of Cdc37 Kazuya Terasawa and Yasufumi Minami Department of Biophysics and Biochemistry, and...

Ngày tải lên: 07/03/2014, 21:20

... [8–13] and Arabidopsis thaliana [14,15]. Yeast, flies, nematodes and mammals encode a single SPO11; however, plants (e.g. Arabidopsis and rice Oryza sativa) encode at least three SPO11 paralogues ... functional DNA -binding activity. TF-SPO11-1 is able to bind to dsDNA and to ssDNA (Fig. 3A, B); however, it possesses approximately twofold higher binding affinity towards dsDNA...

Ngày tải lên: 29/03/2014, 09:20

... chemiluminescence solution. Statistical analysis All experimental data were examined by analysis of vari- ance (anova) procedures, and significant differences among the means from triplicate determinations were assessed using Duncan’s ... are provided by the three SNARE proteins; one each from syntaxin and VAMP2, and two from SNAP-25 [4,5]. Syntaxin and VAMP2 are transmembrane...

Ngày tải lên: 30/03/2014, 04:20

... design of additional salvinorin A analogues. A tentative model is also proposed to explain previous and new data on salvinorin A binding to the opioid receptors. Results and Discussion Chimera Although ... 4-methoxycarbo- nyl points towards TM V and VI, the A ring toward the extracellular side, and the C ring toward the intra- cellular side. The investigators noted tha...

Ngày tải lên: 30/03/2014, 11:20

... receptors, and invertebrate tachykinins: a review. Zool Sci 5, 533–549. 7 Satake H, Ogasawara M, Kawada T, Masuda K, Aoy- ama M, Minakata H, Chiba T, Metoki H, Satou Y & Satoh N (2004) Tachykinin ... vulgaris). Biochem J 387, 85–91. 25 Kanda A, Takahashi T, Satake H & Minakata H (2006) Molecular and functional characterization of a novel gonadotropin-releasing-hormone recep...

Ngày tải lên: 19/02/2014, 00:20

... as cofactor to eliminate the characteristic lag period of tyrosinases [8,20,21]. Figure 3 shows the rate of TH activity as a function of the concentration of l-dopa cofactor added to the assay ... lL10mm sodium periodate was added, and the increase in A 475 immediately determined. A small concentration of l-dopa was occasionally added as cofactor for TH activity when app...

Ngày tải lên: 19/02/2014, 07:20

... the main effects of a Du in speeding up the enzymatic rate are an increased rate o f H + releaseatthecytoplasmicsideandadecreasedrateof H + release at the periplasmic side. Particularly this last effect ... the ATP synthase of Rhodobacter capsulatus results in differential contributions of DpH and Du in driving the ATP synthesis reaction Paola Turina and B. Andrea Melandri Departm...

Ngày tải lên: 21/02/2014, 03:20

... 5¢-AI (A/ C)GIATG (A/ C)GIACIGTIA CIAA(T/C)TA(T/C)TT-3¢ (I represents an inosine residue) and 5¢-CA (A/ G)CA (A/ G)TAIATIGG (A/ G)TT (A/ G)TA CAT-3¢, corresponding to amino-acid sequences RMRTVTNYF (at transmembrane domain II of mamma- lian tachykinin ... TTTTGTgtaaat)146 bpÀcaacagGTATAA Intron 3 AGACGGgtatga)469 bpÀtttcagGTAGTG Intron 4 TGCCAGgtatgt)119 bpÀttccagATTCCG Fig. 5. Schemati...

Ngày tải lên: 21/02/2014, 03:20