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Báo cáo khoa hoc:" A heparin binding synthetic peptide from human HIP / RPL29 fails to specifically differentiate between anticoagulantly active and inactive species of heparin" pptx

Báo cáo khoa hoc:

Báo cáo khoa hoc:" A heparin binding synthetic peptide from human HIP / RPL29 fails to specifically differentiate between anticoagulantly active and inactive species of heparin" pptx

... AccessResearch A heparin binding synthetic peptide from human HIP / RPL29 fails to specifically differentiate between anticoagulantly active and inactive species of heparin David E Hoke1,2, Daniel D Carson3 ... thecoagulation cascade by inactivating serine proteases, suchas factor Xa (FXa) and thrombin. The interaction between ATIII and the Hp pentasaccharide (ATIII binding pen-tasaccharide) is the paradigm ... Previously,it was reported from our laboratories that a 16 amino acid synthetic peptide derived from the C-terminus of human HIP/ RPL29 (HIP peptide- 1) enriched for ATIII-dependent anticoagulant activity,presumably...
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Báo cáo khoa học: A sucrose binding protein homologue from soybean exhibits GTP-binding activity that functions independently of sucrose transport activity pptx

Báo cáo khoa học: A sucrose binding protein homologue from soybean exhibits GTP-binding activity that functions independently of sucrose transport activity pptx

... using the Pfu DNA polymerase, the forward primerS64XHOF 5¢-AAGAAACTCGAGGTCGAAGA-3¢(coordinates 103–121, XhoI site underlined) and the reverseprimer SEF97R 5¢-ATACATTCCCCGAATTCAGCCACCTCC-3¢ (positions ... thio-b-D-galactoside (IPTG). Theinduced protein was affinity-purified using Ni-chelatingSepharose resin (Amersham Pharmacia Biotech.) and usedas an antigen to raise polyclonal antisera in rabbits, ... increase the totalprotein extract as starting material in the binding reactionled to a remarkable increase in the background levelscompromising the quality and interpretation of the data.The...
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Báo cáo khoa học: A client-binding site of Cdc37 ppt

Báo cáo khoa học: A client-binding site of Cdc37 ppt

... 200wt 1A 2A 3A 4A 5A N10M10C10LVCEETANYLVIWCIDLEVEAAAAETANYLVIWCIDLEVELVCEAAAAYLVIWCIDLEVELVCEETANAAAAWCIDLEVELVCEETANYLVIAAAALEVELVCEETANYLVIWCIDAAAALVCEETANYLTANYLVIWCIVIWCIDLEVEFig. 5. Five residues of Cdc37 are essential for the binding ... A client -binding site of Cdc37Kazuya Terasawa and Yasufumi MinamiDepartment of Biophysics and Biochemistry, and Undergraduate Program for Bioinformatics and Systems Biology, Graduate School ... immuno-stained with anti-Myc (for Myc-Knd) and anti-FLAG (for FLAG–GST– peptide) polyclonal antibodies (WB: a- Myc and a- FLAG).K. Terasawa and Y. Minami A client -binding site of Cdc37FEBS Journal...
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Báo cáo khoa học: A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis docx

Báo cáo khoa học: A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis docx

... [8–13] and Arabidopsis thaliana [14,15]. Yeast, flies, nematodes and mammals encode a single SPO11; however, plants(e.g. Arabidopsis and rice Oryza sativa) encode at leastthree SPO11 paralogues ... functional DNA -binding activity. TF-SPO11-1 isable to bind to dsDNA and to ssDNA (Fig. 3A, B);however, it possesses approximately twofold higher binding affinity towards dsDNA than towards ssDNA.As ... 180-bp dsDNA: a major signal of a larger com-plex that was detected in all experiments; and a minorsignal of a smaller complex that was detected only inthe presence of a certain amount of NaCl (Fig....
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Báo cáo khoa học: A search for synthetic peptides that inhibit soluble N-ethylmaleimide sensitive-factor attachment receptor-mediated membrane fusion pot

Báo cáo khoa học: A search for synthetic peptides that inhibit soluble N-ethylmaleimide sensitive-factor attachment receptor-mediated membrane fusion pot

... chemiluminescencesolution.Statistical analysisAll experimental data were examined by analysis of vari-ance (anova) procedures, and significant differences amongthe means from triplicate determinations were assessedusing Duncan’s ... are provided by thethree SNARE proteins; one each from syntaxin and VAMP2, and two from SNAP-25 [4,5]. Syntaxin and VAMP2 are transmembrane proteins with single trans-membrane helices and are ... channelantagonists: a detailed pharmacological study. Neuro-pharmacology 35, 1537–1546.42 Ruiz MA, Clares B, Morales ME, Cazalla S & GallardoV (2007) Preparation and stability of cosmetic formula-tions...
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Báo cáo khoa học: A unique binding epitope for salvinorin A, a non-nitrogenous kappa opioid receptor agonist docx

Báo cáo khoa học: A unique binding epitope for salvinorin A, a non-nitrogenous kappa opioid receptor agonist docx

... design of additional salvinorin A analogues. A tentative modelis also proposed to explain previous and new data onsalvinorin A binding to the opioid receptors.Results and DiscussionChimeraAlthough ... 4-methoxycarbo-nyl points towards TM V and VI, the A ring towardthe extracellular side, and the C ring toward the intra-cellular side. The investigators noted that althoughthere was little atom -to- atom correspondence ... insalvinorin A binding and selectivity and traditional opi-oid ligands that utilize stronger salt–link interactionswith TM III and VI. The idea that salvinorin A wouldprimarily take advantage...
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Tài liệu Báo cáo khoa học: A novel tachykinin-related peptide receptor of Octopus vulgaris – evolutionary aspects of invertebrate tachykinin and tachykinin-related peptide ppt

Tài liệu Báo cáo khoa học: A novel tachykinin-related peptide receptor of Octopus vulgaris – evolutionary aspects of invertebrate tachykinin and tachykinin-related peptide ppt

... receptors, and invertebrate tachykinins: a review. Zool Sci 5,533–549.7 Satake H, Ogasawara M, Kawada T, Masuda K, Aoy-ama M, Minakata H, Chiba T, Metoki H, Satou Y &Satoh N (2004) Tachykinin ... vulgaris).Biochem J 387, 85–91.25 Kanda A, Takahashi T, Satake H & Minakata H (2006)Molecular and functional characterization of a novelgonadotropin-releasing-hormone receptor isolated from the ... Kanda A, Satake H, Kawada T & Minakata H (2005)Novel evolutionary lineages of the invertebrateoxytocin ⁄ vasopressin superfamily peptides and theirreceptors in the common octopus (Octopus...
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Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx

Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx

... as cofactor to eliminate the characteristic lag period of tyrosinases[8,20,21]. Figure 3 shows the rate of TH activity as a function of the concentration of l-dopa cofactor added to the assay ... lL10mmsodium periodate was added, and the increase in A 475immediately determined. A small concentration of l-dopawas occasionally added as cofactor for TH activity whenappropriate (detailed in results). ... crystal structure data.The only data so far available are for sweet potato(Ipomoea batata) catechol oxidase [26]. The catalyticcopper center is accommodated in a central four-helixbundle located...
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Tài liệu Báo cáo khoa học: A point mutation in the ATP synthase of Rhodobacter capsulatus results in differential contributions of DpH and Du in driving the ATP synthesis reaction pptx

Tài liệu Báo cáo khoa học: A point mutation in the ATP synthase of Rhodobacter capsulatus results in differential contributions of DpH and Du in driving the ATP synthesis reaction pptx

... the main effects of a Duin speeding up the enzymatic rate are an increased rate o fH+releaseatthecytoplasmicsideandadecreasedrateofH+release at the periplasmic side. Particularly this lasteffect ... the ATP synthase of Rhodobacter capsulatusresults in differential contributions of DpH and Du in drivingthe ATP synthesis reactionPaola Turina and B. Andrea MelandriDepartment of Biology, Laboratory ... wild-t ype and (m) mutant chromatophores. (A) ACMA fluorescence quenching as a function of time aft eraddition of ATP. The original recorder trac es have been digitalized and data po ints have been...
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Tài liệu Báo cáo khoa học: A novel tachykinin-related peptide receptor docx

Tài liệu Báo cáo khoa học: A novel tachykinin-related peptide receptor docx

... 5¢-AI (A/ C)GIATG (A/ C)GIACIGTIACIAA(T/C)TA(T/C)TT-3¢ (I represents an inosine residue) and 5¢-CA (A/ G)CA (A/ G)TAIATIGG (A/ G)TT (A/ G)TACAT-3¢, corresponding to amino-acid sequencesRMRTVTNYF (at transmembrane domain II of mamma-lian tachykinin ... TTTTGTgtaaat)146 bpÀcaacagGTATAAIntron 3 AGACGGgtatga)469 bpÀtttcagGTAGTGIntron 4 TGCCAGgtatgt)119 bpÀttccagATTCCGFig. 5. Schematic representation of the UTKRcDNA and intron/exon organization of ... 4243PCR anchor primer and a primer (5¢-CGAACACCCAGTGGTTATTCAAC-3¢, complementary to nucleotides693À672), followed by reamplification using the anchorprimer and a primer (5¢-GATATCAAAGCGTCAGCAACTGC-3¢,...
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