Báo cáo khoa hoc:" Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes" ppsx

... 9:8 http://www.jnrbm.com/content/9/1/8 Page 7 of 10 RESEARC H Open Access Shortcomings of short hairpin RNA- based transgenic RNA interference in mouse oocytes Lenka Sarnova 1,2 , Radek Malik 1* , ... short hairpin RNA (shRNA) expression is used to induce RNAi. In contrast, transgenic RNAi in the oocyte routinely employs a long RNA hairpin. Transgenic R...

Ngày tải lên: 11/08/2014, 07:21

... the interdigital regions of limbs, suggesting that Hsp105a may play important roles in apoptosis dur- ing mouse embryogenesis [27]. In the present study, to examine the role of Hsp105a during mouse ... the activation of caspases. These enzymes participate in a cascade that is triggered in response to pro-apoptotic signals and results in cleavage of a set of proteins, r...

Ngày tải lên: 21/02/2014, 03:20

... (Dakopatts Inc., Mississauga, Canada) for two hours at room temperature. After three additional rinses in TPBS, antibody-binding sites were revealed by a 15-minute incubation in 0.2% diaminobenzidine in ... detailed features of this study will extend our current knowledge of α 1B subunit distribution in terms of the number of neuroanatomical areas examined, by investigating α...

Ngày tải lên: 07/08/2014, 15:20

Ngày tải lên: 07/08/2014, 18:21

... prognosis of both groups of patients (Hazard ratio 1.34 and 1.35). Besides this, an interesting result of our investigation is the importance of CT scan preoperative staging both as predictors of survival ... staging > 2; however the importance of fibrinogen value to predict tumour invasion and progno- sis of these patients remains uncertain, given that this marker is not spe...

Ngày tải lên: 09/08/2014, 07:21

... drills according to the standard protocol of the manufacturer. Implants measuring 9 mm in length and 3.5 mm in diameter were inserted using continuous external sterile saline irrigation to minimize ... was induced with an intravenous bolus of 3–5 ml thiopental (Thiopental inresa ® , Inresa Arzneimittel, Freiburg, Germany) followed by intubation and mainte- nance of anaesthesia by in...

Ngày tải lên: 11/08/2014, 20:20

... none of those reports included CRL as a virus-induced gene. The expression of CRL in CaLCuV-infected plants shows an interesting pattern. In terms of the type of response, a short but strong induction, ... D, Hanley-Bowdoin L: A gemin- ivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of inf...

Ngày tải lên: 12/08/2014, 04:20

... This include processing of miRNA and shRNA by Dicer, assembly of resultant guide strand into the RNA- induced silencing complex (RISC), recognition of target viral RNA sequences and cleavage of ... both instruction and facilitating all virus culture aspects of the research, including establishing, selecting, and maintaining all virus strains. SMI was the Principal Investigator a...

Ngày tải lên: 12/08/2014, 04:20

... organization can be found in other genes involved in the biogenesis of the translational machinery including rRNA processing proteins and snoRNAs. Strikingly, the association of telo boxes with site ... boxes in the promoter of genes encoding rRNA processing proteins was examined in Arabidopsis. For 49 genes annotated in the TAIR database as encoding a cytoplasmic rRNA process...

Ngày tải lên: 11/08/2014, 11:21

... briefly addressed in the findings section that follows. Shortly following each interview, the interviewers created a document summarizing the relevant points made dur- ing the interview. Direct ... work in Guate- mala City, so there is a vast shortage of specialists else- where." In explaining the reasons for the lack of Guatemalan specialists working in poor, rural areas, on...

Ngày tải lên: 11/08/2014, 14:21