Báo cáo khoa hoc:" Human haematopoietic stem cells express Oct4 pseudogenes and lack the ability to initiate Oct4 promoter-driven gene expression" ppt

... 2008, 389:845-850. doi:10.1186/1477-5751-9-2 Cite this article as: Redshaw and Strain: Human haematopoietic stem cells express Oct4 pseudogenes and lack the ability to initiate Oct4 promoter-driven gene expression. Journal of Negative ... lack the ability to initiate Oct4 promoter-driven gene expression Zoe Redshaw 1* , Alastair J Strain 2 Abs...

Ngày tải lên: 11/08/2014, 07:21

... that lacks T and B cells, can be transformed into ES cells genetically corrected for the Rag mutation and then turned into blood progenitors that will generate B and T cells when reintroduced into ... performed and demonstrated that the cell line originated from the cloned blastocysts reconstructed from the donor cells, not from parthenogenetic activation. The statisti...

Ngày tải lên: 07/08/2014, 18:21

... from the pCR- BluntII-TOPO-534 vector as template and religation. Then, the KpnI–XhoI digest was isolated and cloned into the pGL3-promoter-Luc reporter vector, leading to the production of the ... in cells expressing these mutants. They also observed the formation of multinucleated cells, centro- some and mitotic spindle abnormalities, and impaired chromosome segrega...

Ngày tải lên: 30/03/2014, 08:20

... stage and then differentiate into blast cells. The bipotentiality of hemangioblast and blast cells provides the opportunity to use them in future cellular therapies of human disorders. Moreover, the ... fetal and embryonic globin genes. Interestingly, these cells expressed also HLA class I molecules, however at very low levels compared to endothelial and hematopoietic...

Ngày tải lên: 18/06/2014, 15:20

... self-renewal, the generation of more identical cells, and developmental potency, the capacity to give rise to more differentiated cells. Stem cells come in different ‘flavors’. Blood-forming (hematopoietic) ... regarding stem cells rests on several converging themes. e derivation of ES cells, first of mouse and then of human origin, provided a platform for the s...

Ngày tải lên: 11/08/2014, 12:21

... mixture of the PLP and PMP forms of the enzyme or of enolimine and ketoenamine tau- tomers of the internal aldimine of PLP [35]. PLP is a probe of the active site environment, and the tauto- meric ... converting the PLP form of the enzyme to the PMP form. Uncompetitive inhibition involves the exclusive (or predominant) binding of the inhibitor to the enzyme– su...

Ngày tải lên: 06/03/2014, 00:21

... one strand and only the 3¢-end G-tract of the other strand [30] (Fig. 3D). The core of this structure, called the (3 + 1) core, has three strands oriented in one direction and one strand oriented ... step toward understanding the structure of the ‘real’ telomeres is to address the question on the structure of long human telomeric DNA sequences [27,37,67,68]. The prob...

Ngày tải lên: 06/03/2014, 09:22

... subtraction. Results Recombinant expression of human anionic trypsinogen The gene for human anionic trypsinogen was cloned under the control of the T7 promoter into the pTrap-T7 expression vector [11,14], that ... have profound effects on the overall stability of the pancreatic trypsinogen pool and its susceptibility to autoactivation. To model these changes in vitro,...

Ngày tải lên: 17/03/2014, 03:20

... in restoration of apoptotic ability of cancer cells. To the best of our knowledge no other interactors of the HBXIP have been reported, nor has its exact A B C Fig. 4. Expression of hSuv3p lacking ... itself can increase the protein stability. For these studies the protein decay rates were measured for the COS-1 cells transfected with the expression vec- tors encoding the...

Ngày tải lên: 23/03/2014, 15:21

... monocytes, airway epithelial cells, reticulocytes and mast cells [6,18–20]. The Th2 cytokines IL-4 and IL-13 induce the expression of 15-LO-1 in monocytes, airway epithe- lial cells and mast cells [20–23]. ... L1236 cells (data not shown). To demonstrate the expression of the 15-LO-1 protein in L1236 cells and the cellular localization of the enzyme in the p...

Ngày tải lên: 30/03/2014, 04:20