Báo cáo y học: "Endotoxin-activated microglia injure brain derived endothelial cells via NF-B, JAK-STAT and JNK stress kinase pathways" docx

... Access Endotoxin-activated microglia injure brain derived endothelial cells via NF-B, JAK-STAT and JNK stress kinase pathways Rachid Kacimi 1 , Rona G Giffard 2 , Midori A Yenari 1* Abstract Background: We previously ... activated microglia, and that this toxicity is likely mediated by reactive nitrogen and oxygen species. LPS activated microglia induce endoth...

Ngày tải lên: 11/08/2014, 06:22

... fibrosis), and S4 (cirrhosis), and grade of disease activity was defined as G0 (no activity), G1 (mild activity), G2 (moderate activity), and G3 (severe activity). All the sections were blindly and ... HBeAg and anti-HBe were measured by en- zyme-linked immunosorbent assay (Livzon Group Reagent Factory, Guangdong, China). HBV DNA The HBV DNA level was determined by quan- titativ...

Ngày tải lên: 25/10/2012, 11:48

... hematopoi- etic cells and lymphocytes. Indeed, CXCL12 is constitutively displayed by endothelial cells (ECs) in the bone marrow and secondary lymphoid organs, where it seems to be produced by nearby osteoblasts, ... cell -derived factor, SDF-1) is constitutively expressed by stromal resident cells and is involved in the homeostatic and inflammatory traffic of leukocytes. Bind...

Ngày tải lên: 09/08/2014, 07:20

... was derived from haematopoetic, neural, or myogenic progenitors or stem cells. Evidence for pericytes in the IPFP tissue and IPFP- derived cells 3G5 distinctively stains pericytes and these cells ... followed by washing and incu- bation for 30 minutes in donkey anti-goat IgG biotin- conjugated secondary antibody (Santa Cruz Biotechnology) for collagen type I and collagen typ...

Ngày tải lên: 09/08/2014, 10:23

... inflammatory cytokines and chemokines. J Inflamm (Lond) 2009, 6:2. 15. Miller MD, Krangel MS: Biology and biochemistry of the chemokines: a family of chemotactic and inflammatory cytokines. Crit ... LPS, poly (I:C), or resiquimod, and/ or TMP, and lysates measured for luciferase activity. RAW 264.7 cells treated with LPS and/ or TMP were used in ChI P and EMSA assays. For ChIP a...

Ngày tải lên: 11/08/2014, 03:20

... Painfu l and repetitive spasms interfere with respiration and may cause metabolic disturbances such as hyperpyrexia, dehydration, respiratory insufficiency, and acute renal failure secondary to rhabdomyolysis. ... generalized dystonia that may occur in patients with primary or secondary dystonia. Painful and repetitive spasms interfere with respiration and may cause metabolic distur...

Ngày tải lên: 11/08/2014, 07:20

... is chosen equally and randomly. The RSCU value for a synonymous codon more than 1.0 or less than 1.0 indicates the more frequency or less frequency, respectively. The synonymous codons with ... equally and randomly. Genetic relationship based on synonymous codon usage in HBV The PCA detected the first principal component (f 1 ’) which can account for 23.65% of the total synonymous .....

Ngày tải lên: 11/08/2014, 21:22

... using cell cycle flow cytometry of CD31 + endothelial cells (Figure 2). An early increase in S-phase cells was noted on Day 3 after pneumonectomy. By Day 6 after pneumonectomy, 10% of the cells in ... cell cycle. To investigate the endothelial response to pneu- monectomy, the lung digests were analyzed by flow cytometry. Baseline analysis by flow cytometry demon- strated 8-15% of th...

Ngày tải lên: 12/08/2014, 13:22

... sex- and age-matched healthy controls. Circulating endothelial cells and endothelial progenitors cells were detected in the peripheral blood of patients with systemic sclerosis by flow cytometry ... blood and to endothelial cells RNA extraction. She also provided the statistical analysis. MD and PA performed the gene array analysis. RA helped in the flow-cytometric analysis...

Ngày tải lên: 12/08/2014, 14:22