... number not for citation purposes) Implementation Science Open Access Research article Moving research into practice: lessons from the US Agency for Healthcare Research and Quality's IDSRN program Marsha ... Interview protocols for AHRQ staff focused on their role in IDSRN, the under- lying rationale for the program, their perspectives on implement...
Ngày tải lên: 11/08/2014, 05:22
... ribose and Ca 2+ into the binding site of SsCU-NH was performed using, as a starting reference, the position of the sugar and of the metal in the crystallo- graphic template 1YOE. The two ligands ... of the program, setting the highest level of optimization. The quality of the mod- els and their stereochemical properties were analyzed using the programs procheck...
Ngày tải lên: 18/02/2014, 17:20
Báo cáo khoa học: Sulfide : quinone oxidoreductase (SQR) from the lugworm Arenicola marina shows cyanide- and thioredoxin-dependent activity pdf
... Dueren, Germany). For cDNA synthesis, the first- strand synthesis kit for RT-PCR (Invitrogen) was used. Hybridization probe, cloning and heterologous expression Standard molecular and biochemical ... substrate other than sulfide and quinone, and, for the SQR from S. pombe, cyanide-independent activity has been described [27]. However, the K m values for sulfide and quino...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Bacterial b-peptidyl aminopeptidases with unique substrate specificities for b-oligopeptides and mixed b,a-oligopeptides pptx
... activities of the two BapA enzymes for the first five peptides were quantified by HPLC of the residual substrate; the reactions of the other substrates were analyzed by measuring the formation of the dipeptide ... exchange chromatography and hydrophobic interaction chromatogra- phy according to the previously published procedure for the wild-type 3-2W4 BapA [17]. The tw...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo khoa học: A novel metallocarboxypeptidase-like enzyme from the marine annelid Sabellastarte magnifica – a step into the invertebrate world of proteases pdf
... glutamic acid from the peptide was observed and, after 60 min, the new C-terminus residues formed and tryptophan and ala- nine were further released, as shown by the trimming scale: 1716, 1587 and 1329 ... isolated from marine invertebrates, and in not one case have the whole or extended sequences been disclosed. This would be the case for the two CPAs and CPBs is...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Recent insights into cerebral cavernous malformations: the molecular genetics of CCM pot
... seemed confusing [1]. Three CCM genes have been mapped and identified in the recent years. These molecular genetics data have provided useful information for clinical care of the patients and were ... vitro studies and in vivo CCM animal model studies have helped to unravel the func- tional roles of these proteins and are be the focus of the two accompanying minireviews by...
Ngày tải lên: 15/03/2014, 10:20
Báo cáo khoa học: Structural insights into mechanisms of non-nucleoside drug resistance for HIV-1 reverse transcriptases mutated at codons 101 or 138 pot
... the only other changes visible are shifts in the position of nevirapine for both RT(Lys101Glu) and RT(Glu138Lys), with the biggest movement observed for the drug in the latter mutant. Previous ... between the carb- oxyl of Glu138 and the inhibitor. It would thus be expected that there is an accompanying reduction in binding affinity for PETT-2 for the Glu138Lys muta...
Ngày tải lên: 16/03/2014, 13:20
báo cáo khoa học: " A typology of practice narratives during the implementation of a preventive, community intervention trial" docx
... gained from the sub- jective meanings of the CDOs (gathered via field diary and interviews) and the concepts they use to understand their practice [21]; much of the CDOs' practice (and program implementation ... of value for adjusting the intervention were raised within memos and in meetings between the EcoPRISM research team and the PRISM research team. T...
Ngày tải lên: 11/08/2014, 16:20
Tài liệu Báo cáo khoa học: "Automatic Collection of Related Terms from the Web" pptx
... from the compiled corpus. 2. Selection by scoring To select the top N (= 30) terms from the list L by using a scoring function. For the scoring function of a term x, we use the following function, ... query. In case the query is a term, its hit is the number of pages that contain the term on the Web. We use the following notation. H(x)= the number of pages that cont...
Ngày tải lên: 20/02/2014, 16:20
Báo cáo khoa học: A new phospholipase A2 isolated from the sea anemone Urticina crassicornis – its primary structure and phylogenetic classification pptx
... placozoans (Trichoplax), and mollusks (Cras- sostrea and Mytilus), and also in the only known vertebrate PLA 2 , that of the sea lamprey (Petromy- zon marinus). Furthermore, the Asn27 PLA 2 s can ... Japan). RACE 5¢-RACE and 3¢-RACE were performed on the RACE- ready cDNA prepared from previously isolated total RNA with the GeneRacer Kit (Invitrogen, Carlsbad, CA, USA). Fo...
Ngày tải lên: 06/03/2014, 11:20