báo cáo khoa học: " Lessons from the evaluation of the UK''''''''s NHS R&D Implementation Methods Programme" doc
báo cáo khoa học: " Lessons from the evaluation of the UK''''s NHS R&D Implementation Methods Programme" doc
... the NHS of the applications submitted, on the progress of commissioned work, and on the quality and value to the NHS of the IMP as it developed. There was some overlap in membership between the ... care. Discussion This evaluation was designed to explore the quality of the outputs of the IMP and of the commissioning process. Our findings regarding the...
Ngày tải lên: 11/08/2014, 05:22
Tài liệu Báo cáo khoa học: Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium doc
... transfer from CDH to cytochrome c, Phe166, which is located in the cytochrome domain and approaches one of propionates of heme, was mutated to Tyr, and the thermodynamic and kinetic properties of the ... the special features of this enzyme. The optimum pH values of flavin reduction by cellobiose (pH 4.5–5.0) and of electron transfer from flavin to heme (pH 3.5–4.0) dif...
Ngày tải lên: 19/02/2014, 18:20
Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf
... these residues stabilize the distortion of the NAG3 residue. In these interac- tions, we particularly focused on the Trp664 residue, which is located at the bottom of the active site cleft. The ... Stereo figures of the model of the bound substrate in the AD2 E526A mutant (A) and D524A mutant (B). The structures of bound sugars and the side-chains of three aci...
Ngày tải lên: 15/03/2014, 11:20
Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
... determined pI of 6.1 and the theoretically deduced pI value of 6.22 was found. A theoretical prediction of the secondary structural organization of Ag-NPA-1 performed on the basis of the amino acid ... cellular distribution of the protein. The binding affinities are characterized by changes in the fluorescence of the single Trp chromophore that is used as a marker...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida pptx
... to that of PMT-C3 is that, at the sequence level, there is no discernible similarity of PMT-C3 to the proteins, with the exception of the Cryseobacterium protein glutaminase. However, there is ... preferential cou- pling of the Ga q versus Ga 11 protein to the down- stream PLCb effector protein. The PMT-induced PLCb response is potentiated by the release of the Ga...
Ngày tải lên: 22/03/2014, 15:21
Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... influence the efficacy of interference RNA in vivo, for example, the length of target mRNA, the length and concentration of dsRNA, the region of homology between the dsRNA and the target, as well as other ... identity with the CHH from other penaeid species. In addition to an identical number of amino acid residues (72), 13 of these were completely positionally conse...
Ngày tải lên: 23/03/2014, 10:20
Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
... the remaining four subunits (NdhH–K) form the connect- ing subcomplex, both of them homologous to the bac- terial subcomplexes. The theoretical molecular mass of the Ndh complex, calculated from ... However, there is not much informa- tion about the existence of the soluble subcomplex (which is the electron input device in bacterial complex I) in the composition of...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf
... stable modification of the protein. However, the elution profiles from the various columns demonstrate that anoxia exposure did not stimulate the synthesis of any new isozymic forms of GST. Based on ... change in the expression pattern of either isozyme during anoxia or of the expression of novel GST isozymes under anoxia. However, the effect of anoxia exposure on...
Ngày tải lên: 23/03/2014, 15:20
Báo cáo khoa học: Solution structure and stability of the full-length excisionase from bacteriophage HK022 pot
... to other proteins of this class, such as the DNA-binding domain of the Mu repressor [21]. Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... functions obtained under the same conditions as the NMR experi- ments (Fig. 2). Direct comparison of the thermodynamic parameters of Xis_wt and Xis_C28S indicated that...
Ngày tải lên: 23/03/2014, 15:21
Báo cáo khoa học: "Structural and functional characterization of the 5’ upstream region of a glutamine synthetase gene from Scots pine" pdf
... into the plasmid pGEM-3Z to generate the clone pGS217 and used for analysis and sequencing. 2.2. Fusions of the 5’region of GS1a to the GUS reporter gene The 981 bp sequence upstream of the translation ... the isolation and characterization of a genomic clone encoding Scots pine (P. sylvestris) cytosolic glutamine synthe- tase GS1a. The clone contains the 5’ half of...
Ngày tải lên: 08/08/2014, 14:20