... clonedenzyme is somewhat toxic to E. coli cellsandwouldhelp to explain why family 48 cellulases are more difficult to clone, with only 12 family members identified up to now[11].Ó FEBS 2003 Cellobiohydrolase ... rho-inde-pendent terminator and with no similarity to previouslydescribed operators was found 20 nucleotides downstreamthe stop codon of cel9B and 28 nucleotides upstream thehypothetical promoter ... homology to Clostridium speciescellulases [20,21], and a reducing-end processive cello-biohydrolase (Cel48C), similar to those found in anaerobicbacteria [34], seem to be closer to the cellulolytic...