... N., Nakano, S., Katoh, M., Matsumura, A. ,Nakamuta, H., Ohmichi, T., Yoneyama, M. & Sasaki, M. (1995)Thermodynamic parameters to predict stability of RNA/DNAhybrid duplexes. Biochemistry 34, ... the thermodynamic parametersto 37 °C is completely necessary. In this case, Eqns (6) and (7) provide a reasonable and valid way to estimate thethermodynamic parameters at the temperature of interest.With ... contrast to theenthalpy and entropy changes, the free energy change and melting temperature are relatively insensitive to the heatcapacity change. Finally, the 16 NN-model free energyparameters...