báo cáo khoa học: "Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo" pptx

... domain of integrins binds directly to ECM proteins, the cytoplas- mic tail is linked to the actin cytoskeleton via signalling and adapter proteins, such as focal adhesion kinase (FAK), vinculin, ... spectrally distinguished within the cell. Therefore, the intein-mediated approach for simultaneous, in vivo, site-specific (N- and C-terminus) conjugation of Quantum Dots to...

Ngày tải lên: 11/08/2014, 00:23

... purposes) In vivo conjugation of QD's to Akt-PH-EGFP via intein mediated protein splicingFigure 1 In vivo conjugation of QD's to Akt-PH-EGFP via intein mediated protein splicing. (a) ... microinjection together with the I C -QD's. In vivo association of the intein halves in the cytosol triggered protein trans-splicing, resulting in the ligation of...

Ngày tải lên: 11/08/2014, 00:22

... [27]. Substrate binding adjacent to the Fe(II) is proposed to weaken binding of the remaining coordinated water, thus enabling the binding of oxygen [28,29]. Oxidative decarboxylation of 2OG then ... were obtained at 75 s intervals and integrated using absolute intensity scaling to monitor changes in the intensity of sig- nals of interest. Synthetic hydroxylated CODD is iden...

Ngày tải lên: 23/03/2014, 03:20

... a sim- ple linear model passing through the origin. Original article Linear and non-linear functions of volume index to estimate woody biomass in high density young poplar ... can be noted that, in the linear model, the order of magnitude of the intercept is 8 to 46 g. This results in a poor estimation of the mass of small stems. T...

Ngày tải lên: 08/08/2014, 18:21

... subsequently stained for TMAO reductase (TorA) activity. Asterisk denotes slower-migrating cytoplasmic form of TorA. (B) A TorA-GFP con- struct was expressed in DtatABCDE cells using an arabinose- inducible ... DtatABCDE expressing pBAD-ABC or the same vector containing mutations in both TatA and TatB as indica- ted, were assayed for export of TorA using the protocol described in...

Ngày tải lên: 19/02/2014, 17:20

... -best lists for a single (and often small) value of are only snapshots and likely to be misleading. For instance, considering the set of AdjN base data with we might arrive at the following results ... significantly 10 different from the baseline obtained by random selection. In contrast to our expectation stated at the be- ginning of this section, the performance of and rela...

Ngày tải lên: 20/02/2014, 18:20

... query- triples and indexing-triples making use of a domain-specific ontology. 1 Indexing and Retrieval of Photographs The normal practice in human indexing or cata- loguing of photographs is to use a text-based ... the starting point for indexing. We have focused on the devel- opment and implementation of automatic caption- based techniques for indexing and retrieval of pho-...

Ngày tải lên: 22/02/2014, 02:20

... alignment of MBP(74–85) with several s pecies of MBP indicates the important role of Arg78, firstly in the stabilization of local microdomains (e pitopes) of the integral protein and in a number of post-translational ... adaptability of bovine myelin basic protein. Proteins 7, 32–40. 34.Ramwani,J.J.,Epand,R.M.&Moscarello,M.A.(1989) Secondary structure of charge isome...

Ngày tải lên: 07/03/2014, 16:20

... would be to evaluate the time necessary for reduction to 50% of initial hormone level, what we defined as HR, and to consider it in the definition of HC. Choi et al. observed HR in 35 of the 42 patients ... evaluation of imaging studies, it is necessary to use greater amounts of information with respect to any prior surgeries performed. Meij et al. reported a higher...

Ngày tải lên: 09/08/2014, 09:20

... snapping sound and intermittent pain involving her right knee. A physical examination at this time revealed mild tenderness to the lateral joint line, butallothertestresultsandfindingsfromplainradio- graphs ... result in compl ete healing of an osteochondral lesion. Non-surgical treatment including activity modification is primarily indicated for stable juvenile OCD. It may include cr...

Ngày tải lên: 10/08/2014, 23:20