báo cáo khoa học: " Atomic Force Microscope nanolithography on chromosomes to generate single-cell genetic probes" doc

... 9:27http://www.jnanobiotechnology.com/content/9/1/27Page 5 of 7RESEA R C H Open Access Atomic Force Microscope nanolithography on chromosomes to generate single-cell genetic probesSebastiano Di Bucchianico1*, ... regions, identifiedby topographic banding, to perform the nanodissectionexperiments. Upon localization of the chromosomeregion to be dissected the AFM microscope is switchedin Contact Nanolithography ... libraries and to the molecular cytogenetic mapping related to the investigationof genetic diseases.BackgroundThe co nventional approach to chromosomes micr odis-section is based on the use of...

Báo cáo khoa hoc:" Human spongiosa mesenchymal stem cells fail to generate cardiomyocytes in vitro" doc

... differentiation of hMSCs, we used the combinationof AZA (an inhibitor of DNA methylation) and TSA (aninhibitor of histone deacetylases) prior to start of cardiacdifferentiation protocols in vitro. ... hypermethylation and chromatincompaction by histone deacetylation are proved to beimportant mechanisms by which some gene expressioncan be silenced in stem cells [18,19]. To push the sponta-neous ... dose-escalationcontribution of transplanted MSCs into formation ofpathological encapsulated structures containing calcifica-tions and ossifications within infarcted myocardiumclearly demonstrated...

báo cáo khoa học: "Atomic force microscopy: a powerful tool for high-resolution imaging of spermatozoa" ppt

... fixation like in elec-tron microscopy. Conventional microscopy not only dis-torts sperm morphology, but is also unable to providehigh-resolution 3D images owing to the small size of thespermatozoa. ... axoneme region inaddition to depressions of the membrane that could notbe observed with the conventional microscope has beenreported. The 3D image contrast mechanism has been uti-lized to ... function of the microtubule is predicted on thebasis of the 3D movement of dynein motors i.e., a motionin a direction at a right angle to the longitudinal axis ofaxonemes. This has been confirmed...

Báo cáo khoa học: The effect of heme on the conformational stability of micro-myoglobin doc

... 89–96 ª 2007 The Authors Journal compilation ª 2007 FEBS 89found to be prone to aggregation and precipitation athigh concentration. By contrast, a longer Mb-derivedfragment, spanning residues ... simulations were performed at 400 K. During thesimulations, the non-bonded interaction cut-off was set to 12 A˚, which is sufﬁciently large to include long-range elec-trostatic interactions. ... seems to stabilize the lMb foldedstate also by counteracting long-range electrostaticinteractions that would otherwise act as destabilizingforces for native-like conformations.ConclusionsThe...

Báo cáo khoa học: "Using a Randomised Controlled Clinical Trial to Evaluate an NLG System" doc

... personalised oral communication withsmokers, but none on personalised written com-munication, because no one currently writes per-sonalised letters to smokers). In such applica-tions, the only ... STOP letter generated from the Figure 1 questionnaireInformation for Stopping SmokingDo you want to stop smoking?Everyone has things they like and dislike about their smoking. Thedecision ... Smoking Information for Heather StewartYou have good reasons to stop People stop smoking when they really want to stop. It is encouraging thatyou have many good reasons for stopping. The scales...

Báo cáo khoa học: Trypanosoma brucei: a model micro-organism to study eukaryotic phospholipid biosynthesis docx

... phosphoglycerolmodiﬁcation of eukaryotic elongation factor 1A [44].It is worth mentioning that the dependence of GPIand ethanolamine phosphoglycerol synthesis on PE asthe ethanolamine donor was demonstrated ... beenreported, one localizing to the ER and one to the Golgi [89]. Thecorresponding model proposes that the ER enzyme preferentiallyuses inositol formed de novo from glucose-6-phosphate to generate ... and protozoa [103–107].The biosynthesis of sphingo(phospho)lipids startswith the condensation of l-serine with palmitoyl-CoA to form 3-ketosphinganine, a reaction catalysed byserine palmitoyltransferase....

Báo cáo khoa học: Application of the Fc fusion format to generate tag-free bi-speciﬁc diabodies doc

... phosphate solution (pH 6.0).Preparation of tag-free hEx3 diabodies from theFc fusion format To construct the expression vector for preparing tag-freediabodies by using IgG-like BsAbs, we connected ... wasdissolved to saturation in water:acetonitrile (2 : 1 v ⁄ v) con-taining 0.067% triﬂuoroacetic acid. Sample solutionsfrom each stage were mixed with the sinapic acid-saturatedsolution in a 1 ... Journal compilation ª 2009 FEBSApplication of method to tag-free bi-speciﬁc sin-gle-chain diabody To demonstrate the utility of this novel method, weapplied it to the preparation of tag-free...

Báo cáo khoa học: "Word or Phrase? Learning Which Unit to Stress for Information Retrieval∗" doc

... ‘tropical storms’, retrieving a documentonly containing tropical can be meaningless, but adocument about storm can be meaningful. Based on this observation, we only incorporate documentfrequency ... arepotentially useful for not only discriminating aphrase itself but characterizing its constituents. Inthis section, we report only the ones that havemade positive contributions to the overall retrievalperformance. ... evidence that ithas to be considered in phrase weighting. It seems to be a natural conclusion, because the importanceof the modiﬁer word in retrieval is subordinate to the relation to its headword,...

Báo cáo khoa học: "An Unsupervised Dynamic Bayesian Network Approach to Measuring Speech Style Accommodation" doc

... normalized10-bin histograms for the 7 features were concate-nated to result in a single 70-dimensional obser-vation vector for the turn. These 70 dimensionalobservation vectors for each turn ... accommodation along a decreolizing contin-uum. International Journal of the Sociology of Lan-guage, 1984(46), 71–86.Putman, W. & Street Jr, R. (1984). The conceptionand perception of noncontent ... Relation of ex-perimentally manipulated interpersonal perceptionand psychological differentiation to the temporalpatterning of conversation. In Proceedings of the78th Annual Convention of...

Báo cáo khoa học: Atomic-resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion pot

... positions. The latterresidue has a gauche conformation along the Cb–Ccbond (torsion angle Ca–Cb–Cc–Sdof 50.0°, 48.2° and50.4° in molecules A, B and C, respectively). As inother cytochrome ... is adjacent to the secondheme ligand, Met65, and Pro66 undergoes conforma-tional changes upon oxidation ⁄ reduction [16]. Whereasin cyanobacterial, plant and red and brown algal cyto-chrome ... 2FoÀ Fcelectron density contoured at 1.2r.W. Bialek et al. Atomic- resolution structure of cytochrome c6FEBS Journal 276 (2009) 4426–4436 ª 2009 The Authors Journal compilation ª 2009 FEBS...

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