báo cáo khoa học: "Design and characterization of protein-quercetin bioactive nanoparticles" doc

... spectra of BSA, Ly s, and Mbsystem. The concentration of (A and B) BSA, (A ’ and B’) Lys, or (A’’ and B’’) Mb was 1.5 × 10 -5 mol/L. (A), (A’), and (A’’) Effects of DMSO at 27°C. (B), (B’), and (B’’) ... 9:19 http://www.jnanobiotechnology.com/content/9/1/19 Page 10 of 14 Figure 6 shows the variat ion of the zeta po tential of the BSA, Lys, and Mb systems versus the c...

Ngày tải lên: 11/08/2014, 00:23

... similarity of the A- and B-chains of the four isoforms, a pairwise alignment was performed and the values of identity expressed in Fig. 3. (Continued). Characterization of four pulchellin isoforms ... M r values of the standards in thousands. Lanes 2–9, 5 lg of peak P I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec- tively, in the presence (lanes 2–5) o...

Ngày tải lên: 18/02/2014, 16:20

... Buchert 1 and Kristiina Kruus 1 1 VTT Technical Research Centre of Finland, Espoo, Finland 2 Protein Chemistry Research Group and Core Facility, Institute of Biotechnology, University of Helsinki, ... 2-aminophenol docking at a model of tyrosinase active site. New J Chem 27, 909–913. 62 Toussaint O & and Lerch K (1987) Catalytic oxidation of 2-aminophenols and ortho hydr...

Ngày tải lên: 19/02/2014, 06:20

... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O). Panel M is the control ... 7A, D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nuc- leus, and Fig. 7C, F, K and N are the merged images of A and B, D an...

Ngày tải lên: 19/02/2014, 07:20

... evidence for existence of two distinct HSF1 isoforms in rainbow trout and the formation of heterotrimers of these isoforms in vitro. Materials and methods Cell culture and animals Rainbow trout ... and characterization of two distinct isoforms of rainbow trout heat shock factor 1 Evidence for heterotrimer formation Nobuhiko Ojima and Michiaki Yamashita Cell Biology Secti...

Ngày tải lên: 19/02/2014, 12:20

... out some of the details of the implementation of this model. OVERVIEW OF THE PROBLSM One of the well-known characteristic features of natural languages is the size and the complexity of their ... processing program that must be able to handle very large amounts of text necessitates dictionaries of substantial size, of the order of at least tens of thousands o...

Ngày tải lên: 22/02/2014, 09:20

... parameters and transport characteristics of Bip-[ 3 H]Pro. Results and Discussion Synthesis, chemical characterization and stability of Bip-Pro Figure 1 shows the structure (Fig. 1A) and the synthe- sis ... a competitive mode of action. Uptake of Bip-[ 3 H]Pro by SKPT cells After characterization of Bip-Pro as a very high-affin- ity and enzymatically stable substrate o...

Ngày tải lên: 07/03/2014, 05:20

... amide and carbonyl of Cys140 form hydro- gen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of the active site cavity and the replacement of which ... cloning site of the vector by digestion with NcoI and PstI. This vector backbone was excised from an agarose gel and purified. For both standard and error-prone PCR amplificati...

Ngày tải lên: 07/03/2014, 11:20

... of ml1p variants giving 169 and 246 bp fragments. SalI cuts the amplification product of ml2p, giving 66, 121 and 243 bp fragments, and the amlpification product of ml3p and ml3.1p, giving 50 and ... AflII, NsiI(andBclI, data not shown) digests gave a very similar pattern of five bands, one of which was also more intensive than the others. Thus, the different intensity of th...

Ngày tải lên: 07/03/2014, 15:20

... and 215 nm, indicating that Rv1399c is composed of 22% o f a-helices, 25% of b-strand a nd 39% o f random coil. The catalytic triad is located at the bottom of a small pocket, 20 A ˚ length and ... Model of Rv1399c. (A) Ribbon diagram of Rv1399c model. The central b-sheet and surrounding a-helices are shown in green and red, respectively. Residues of the catalytic triad (S...

Ngày tải lên: 07/03/2014, 16:20